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Specialty: Biology
Source: Life Sciences
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Total 146 results found since Jan 2013.
CRIP1 promotes cell migration, invasion and epithelial-mesenchymal transition of cervical cancer by activating the Wnt/ β‑catenin signaling pathway
In conclusion, CRIP1 promotes cell migration and invasion, mediates EMT and activates the Wnt/β‑catenin signaling pathway in CC.
Source: Life Sciences - July 4, 2018 Category: Biology Source Type: research
Fibulin-1 interacts with Sex Hormone Binding Globulin and is linked to less aggressive estrogen-dependent breast cancers
In this study we investigated the expression of fibulins, their functional relationship with SHBG and involvement in behavior of estrogen-sensitive breast cancer. Main methods Gene expression of fibulins was performed by Real time-PCR on two estrogen-sensitive breast cancer cell lines, MCF-7 and T47D. Fibulin-1 protein expression and localization were determined by Western blot and immunofluorescence. SHBG interaction with-fibulin-1 was assessed by GST-pull down assay. MCF-7 cell growth and gene expression, after fibulin-1 silencing by siRNA, were evaluated. Finally, the expression of fibulin-1 was correlated to clinical a...
Source: Life Sciences - July 1, 2018 Category: Biology Source Type: research
Neuralized1a regulates asymmetric division in mouse Lewis lung carcinoma cells
Publication date: 1 August 2018 Source:Life Sciences, Volume 206 Author(s): Liangsheng Kong, Jingyuan Li, Yongli Liu, Zhiwei Sun, Shixia Zhou, Junling Tang, Ting Ye, Jianyu Wang, H. Rosie Xing Asymmetric division (ASD), the unique characteristic of normal stem cells, is regarded as a stemness marker when applied to the study of cancer stem cells (CSCs). However, the role of ASD in the self-renewal of CSCs and its regulation remain largely unknown. Here, we first established a mouse Lewis lung carcinoma CSC cell line that could undergo asymmetric division (LLC-ASD cells) derived from the parental mouse Lewis lung carcinoma...
Source: Life Sciences - May 24, 2018 Category: Biology Source Type: research
Slit2-Robo2 signaling modulates the fibrogenic activity and migration of hepatic stellate cells
Publication date: 15 June 2018 Source:Life Sciences, Volume 203 Author(s): Zhiping Zeng, Yujing Wu, Yirong Cao, Ziying Yuan, Yuanqing Zhang, David Y. Zhang, Daisuke Hasegawa, Scott L. Friedman, Jinsheng Guo Background & aim Slit/Robo signaling was originally identified as a repulsive guidance cue in regulating axon branching and neuronal migration. Hepatic stellate cells (HSCs) are the key fibrogenic cells in the liver, which are migratory when activated, and express neural crest markers. The aim of the present study was to investigate the functional significance of Slit/Robo signaling in liver fibrogenesis and in...
Source: Life Sciences - May 18, 2018 Category: Biology Source Type: research
microRNA-136 inhibits proliferation and promotes apoptosis and radiosensitivity of cervical carcinoma through the NF- κB pathway by targeting E2F1
Publication date: 15 April 2018 Source:Life Sciences, Volume 199 Author(s): Hong-Jie Lu, Pei-Ying Jin, Yao Tang, Shao-Hua Fan, Zi-Feng Zhang, Fang Wang, Dong-Mei Wu, Jun Lu, Yuan-Lin Zheng Aims Several microRNAs (miRs) are expressed aberrantly and associated with progression, tumorigenesis, and prognosis of haematological and solid tumors. The study aimed to identify the effects involved with microRNA-136 (miR-136) on the concurrent enhancement of proliferation, apoptosis and radiosensitivity of cervical carcinoma through the NF-κB signaling pathway by targeting E2F1. Main methods Totally 338 patients with cervical carci...
Source: Life Sciences - March 27, 2018 Category: Biology Source Type: research
Heme oxygenase-1 protects liver against ischemia/reperfusion injury via phosphoglycerate mutase family member 5-mediated mitochondrial quality control
Publication date: Available online 8 March 2018 Source:Life Sciences Author(s): Jeong-Min Hong, Sun-Mee Lee Aims Heme oxygenase-1 (HO-1), an endogenous cytoprotective enzyme, is reported that can be localized in mitochondria under stress, contributing to preserve mitochondrial function. Mitochondrial quality control (QC) is essential to cellular health and recovery linked with redox homeostasis. Recent studies reported that phosphoglycerate mutase family member (PGAM) 5, a mitochondria-resident phosphatase, plays critical role in mitochondrial homeostasis. Therefore, we aim to investigate cytoprotective mechanisms of HO-1...
Source: Life Sciences - March 8, 2018 Category: Biology Source Type: research
Silencing calreticulin gene might protect cardiomyocytes from angiotensin II-induced apoptosis
Publication date: 1 April 2018 Source:Life Sciences, Volume 198 Author(s): Wen Zhang, Zhiyue Liu, Yanmei Zhang, Qinxue Bao, Wenchao Wu, He Huang, Xiaojing Liu Aims Calreticulin (CRT), as a chaperone, contributes to protein folding and quality control cycle. CRT is an important factor regulating Ca2+ that participates in cell apoptosis. However, the function of CRT in the heart is still controversial. Therefore, we aimed to investigate the potential role of CRT in angiotensin II-induced cardiomyocytes apoptosis. Main methods Primary cultured neonatal cardiomyocytes were stimulated with angiotensin II to induce the apoptosi...
Source: Life Sciences - March 3, 2018 Category: Biology Source Type: research
In vitro study of FUZ as a novel potential therapeutic target in non-small-cell lung cancer
Publication date: Available online 6 February 2018 Source:Life Sciences Author(s): Minwei He, Kangqi Li, Chuanfei Yu, Bingfeng Lv, Ning Zhao, Jinhai Deng, Lulu Cao, He Huang, Ang Yin, Taiping Shi, Lu Wang FUZ is regarded as a planar cell polarity effector that controls multiple cellular processes during vertebrate development. However, the role of FUZ in tumor biology remains poorly studied. Our purpose of this study is to discover the physiological effects and mechanism of FUZ in non-small-cell lung cancer (NSCLC) in vitro. With the help of bioinformatics analysis, we noticed that the expression level of FUZ negatively c...
Source: Life Sciences - February 7, 2018 Category: Biology Source Type: research
TNF- α increases Staphylococcus aureus-induced death of human alveolar epithelial cell line A549 associated with RIP3-mediated necroptosis
Publication date: 15 February 2018 Source:Life Sciences, Volume 195 Author(s): Shun-hang Wen, Luo-na Lin, Hu-jun Wu, Lu Yu, Li Lin, Li-li Zhu, Hai-yan Li, Hai-lin Zhang, Chang-chong Li Aim To explore the role of tumor necrosis factor-alpha (TNF-α) on Staphylococcus aureus-induced necroptosis in alveolar epithelial cells. Main methods The A549 alveolar epithelial cell line was pretreated with small interfering RNA (siRNA) against receptor interacting protein-3 (RIP3) and then stimulated by S. aureus, where some cells were pretreated with TNF-α or TNF-α with anti-TNF-α antibody simultaneously. A549 cell death was assess...
Source: Life Sciences - January 12, 2018 Category: Biology Source Type: research
Knockdown of HIF-1 α inhibits the proliferation and migration of outer root sheath cells exposed to hypoxia in vitro: An involvement of Shh pathway
This study is performed to investigate whether hypoxia-induced elevation of hypoxia-inducible factor (HIF)-1α, a transcriptional activator, contributes to the outgrowth of ORS cells in vitro. Main methods Hair follicles with intact ORS collected from 4-month old male American minks were cultured in normoxic or hypoxic condition (3% oxygen) for 7days. Primary ORS cells isolated from the mink hair follicles were exposed to hypoxia for 12, 24 or 48h, and their proliferation was analyzed with immunofluorescence assay using anti-proliferating cell nuclear antigen (PCNA) antibody. The migratory ability of ORS cells was detected...
Source: Life Sciences - November 27, 2017 Category: Biology Source Type: research
PP2A regulates SCF-induced cardiac stem cell migration through interaction with p38 MAPK
Publication date: 15 December 2017 Source:Life Sciences, Volume 191 Author(s): Ying Wang, Yanli Xia, Dong Kuang, Yaqi Duan, Guoping Wang Aims Previous studies have shown that stem cell factor (SCF) induces the migration of cardiac stem cells (CSCs) and helps to repair myocardial infarctions. Earlier studies on the migration mechanism only focused on the activation of kinases; here, we aimed to explore the functional role of protein phosphatase 2A (PP2A) in SCF-induced CSC migration. Main methods CSCs were treated with SCF, PP2A enzymatic activity was measured, the phosphorylation levels of PP2A, p38 MAPK and cofilin were ...
Source: Life Sciences - October 14, 2017 Category: Biology Source Type: research
Down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer cells by up-regulating EN2
This study aimed to investigate the role of miR-605 and EN2 in prostate cancer (PCa). Materials and methods In this research, the expression of miR-605 and EN2 protein in PCa tissues and cells were determined by qRT-PCR and western blot, respectively. The cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and the tumor cell invasion assay was accomplished with transwell system. Flow cytometry was used to analyze the cell cycle. The endogenous expression of miR-605 and EN2 was modulated by recombinant plasmids and cell transfection. Dual luciferase reporter assay was performed to determine the interaction betwee...
Source: Life Sciences - September 28, 2017 Category: Biology Source Type: research
Osteopontin plays a unique role in resistance of CD34+/CD123+ human leukemia cell lines KG1a to parthenolide
Conclusion Parthenolide in spite of the reduction in gene expression of AKT, mTOR or beta-catenin, stimulates the OPN expression in KG1a cells. The OPN expression pattern in KG1a cells could be compatible with CD34+/CD123+ subtype enrichment by PTL which in turn implies OPN's unique role in resistance of cell populations characterized by CD34+/CD123+ phenotype.
Source: Life Sciences - September 23, 2017 Category: Biology Source Type: research
SOCS2 exacerbates myocardial injury induced by ischemia/reperfusion in diabetic mice and H9c2 cells through inhibiting the JAK-STAT-IGF-1 pathway
This study aimed to investigate potential candidates and molecular mechanisms of myocardial ischemia/reperfusion (I/R) injury (MIRI) in type 2 diabetes mellitus. Main methods Type 2 diabetic and myocardial I/R mouse models were established with a high fat-diet (HFD) for 24weeks and subjecting to global ischemia/reperfusion for 1h/3h, respectively. Microarray analysis was applied to screen differentially expressed genes (DEGs) in the hearts of these mice. Moreover, H9c2 cells were treated with high glucose (HG) and/or hypoxia and reoxygenation (H/R). Subsequently, the expression of suppressor of cytokine signaling 2 (SOCS2)...
Source: Life Sciences - September 8, 2017 Category: Biology Source Type: research
Glucocorticoids regulate MiR-29c levels in vascular smooth muscle cells through transcriptional and epigenetic mechanisms
The objective of this study was to determine the underlying mechanism by which glucocorticoids (GCs) induce of miR-29c expression in vascular smooth muscle cells. Main methods QRT-PCR was used for miR-29c detection. Protein levels were determined by western blotting. Knockdown of SP1, DNMT1 and DNMT3A was achieved through transfection with their specific respective siRNAs. The effect of GCs on SP1 activity was determined by luciferase reporter assay and the methylation status in miR-29c promoter was detected by methylation specific PCR. CHIP assay was used to determine the binding ability of SP1 and glucocorticoid receptor...
Source: Life Sciences - August 12, 2017 Category: Biology Source Type: research
