Filtered By:
Specialty: Biology
Source: Life Sciences
This page shows you your search results in order of date. This is page number 3.
Order by Relevance | Date
Total 146 results found since Jan 2013.
Mitochondrial pyruvate carrier 2 mediates mitochondrial dysfunction and apoptosis in high glucose-treated podocytes
Publication date: Available online 10 October 2019Source: Life SciencesAuthor(s): Jun Feng, Yiqiong Ma, Zhaowei Chen, Jijia Hu, Qian Yang, Guohua DingAbstractAimsPodocytes play an important role in the development of diabetic kidney disease (DKD). Mitochondria are the source of energy for cell survival, and mitochondrial abnormalities have been shown to contribute to podocyte injury in DKD. In high glucose (HG)-treated podocytes, mitochondrial function and dynamics are abnormal, and intracellular metabolism is often disrupted. However, the molecular mechanism is still unclear. Mitochondrial pyruvate carrier 2 (MPC2) mediat...
Source: Life Sciences - October 10, 2019 Category: Biology Source Type: research
LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299-3p/FGF2 axis
In conclusion, our results revealed that RHPN1-AS1 promoted cervical cancer progression via targeting miR-299-3p/FGF2 axis. Our data suggested that RHPN1-AS1/miR-299-3p/FGF2 axis may be a promising target for cervical cancer treatment.
Source: Life Sciences - September 14, 2019 Category: Biology Source Type: research
Depletion of CPEB1 protects against oxidized LDL-induced endothelial apoptosis and inflammation though SIRT1/LOX-1 signalling pathway
Publication date: Available online 12 September 2019Source: Life SciencesAuthor(s): Kaicheng Xu, Xiwen liu, Guanghao Ren, Dexin Yin, Suli Guo, Yue ZhaoAbstractAtherosclerosis (AS) is a chronic inflammatory disease that results from Oxidized low-density lipoprotein (Ox-LDL) induced endothelial dysfunction. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is closely related to the development of epithelial cells, but the role of CPEB1 in AS remains unknown. The RNA and protein levels of CPEB1 expression are increased by Ox-LDL exposure, which is abrogated by c-Jun amino-terminal kinase (JNK) inhibitor SP600125. ...
Source: Life Sciences - September 13, 2019 Category: Biology Source Type: research
Proteomics evaluation of MDA-MB-231 breast cancer cells in response to RNAi-induced silencing of hPTTG
This study aims to evaluate cell proliferation and the downstream expression pattern of hPTTG1 gene at the mRNA and protein levels after specific down-regulation of hPTTG1 by siRNA.Main methodsThe human breast cancer MDA-MB-231 cell line was transfected with siRNA against hPTTG1. The mRNA and protein expression levels were examined by Real-time PCR and Western blot, respectively. The cell proliferation was assayed by MTS. To investigate the pattern of protein expression, total cellular protein was analyzed by 2D gel electrophoresis and mass spectroscopy. Subsequently, the possible biological consequences were determined by...
Source: Life Sciences - September 13, 2019 Category: Biology Source Type: research
Dimethyl fumarate attenuates lipopolysaccharide-induced mitochondrial injury by activating Nrf2 pathway in cardiomyocytes
Publication date: Available online 9 September 2019Source: Life SciencesAuthor(s): Chun-Yan Fu, Jun Chen, Xiao-Yang Lu, Ming-Zhi Zheng, Lin-Lin Wang, Yue-Liang Shen, Ying-Ying ChenAbstractAimsTo determine whether dimethyl fumarate (DMF) can protect against lipopolysaccharide (LPS) -induced myocardial injury.Main methodsH9c2 cells pretreated with or without DMF were stimulated with LPS. Cell viability and apoptosis were evaluated. Nrf2 and HO-1 expression were detected using Western blotting. Mitochondrial morphology, mitochondrial and superoxide production were observed using confocal microscope. Mitochondrial respiration ...
Source: Life Sciences - September 10, 2019 Category: Biology Source Type: research
Circular RNA 4099 aggravates hydrogen peroxide-induced injury by down-regulating microRNA-706 in L02 cells
This study aimed to explore the roles and regulatory mechanisms of the circRNA-4099 in L02 cell line.Main methodsCells were induced with H2O2 dilutions and transfected with circRNA-4099 overproduction vectoer and the specific siRNA (si-circRNA-4099), as well as microRNA-706 (miR-706) mimic or the corresponding controls. Cell viability was detected with the CCK-8. The apoptotic rate was determined by the annexin v-FITC/PI with flow cytometer. The expression of circRNA-4099 or miR-706 was quantified depending on qRT-PCR. The reactive oxygen species (ROS) level was examined through ROS assay. The relative proteins were indivi...
Source: Life Sciences - September 1, 2019 Category: Biology Source Type: research
Effects of MFHAS1 on cognitive impairment and dendritic pathology in the hippocampus of septic rats
Publication date: Available online 30 August 2019Source: Life SciencesAuthor(s): Jing Zhong, Chenyue Guo, Wenting Hou, Na Shen, Changhong MiaoAbstractAimsTo investigate the effects of malignant fibrous histiocytoma amplified sequence 1 (MFHAS1) on cognitive dysfunction, the expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and amyloid β peptide (Aβ) in the hippocampus, as well as dendritic pathology in the hippocampal CA1 region in sepsis-associated encephalopathy (SAE) rats.Main methodsThe rats were randomly divided into four groups: 1) control group (subjected to sham surgery), 2) control plus Mfhas...
Source: Life Sciences - September 1, 2019 Category: Biology Source Type: research
Downregulation of hsa_circ_0007534 restricts the proliferation and invasion of cervical cancer through regulating miR-498/BMI-1 signaling
Publication date: Available online 21 August 2019Source: Life SciencesAuthor(s): Xuan Rong, Wei Gao, Xiaomei Yang, Junjun GuoAbstractAimsHsa_circ_0007534 has been reported to be a novel cancer-related circRNA affecting multiple cancers. However, little is known about the role of hsa_circ_0007534 in cervical cancer specifically. In the current study, we aimed to explore the expression and function of hsa_circ_0007534 in cervical cancer.Main methodsThe expression of circRNA, miRNA and mRNA was measured using real-time quantitative polymerase chain reactions. Protein expression was assessed by Western blot. Knockdown of has_c...
Source: Life Sciences - August 22, 2019 Category: Biology Source Type: research
Klotho improves diabetic cardiomyopathy by suppressing the NLRP3 inflammasome pathway
Publication date: Available online 15 August 2019Source: Life SciencesAuthor(s): Xuelian Li, Zhiyang Li, Bingong Li, Xianjie Zhu, Xingjun LaiAbstractAimsNLRP3 inflammasome activation is essential for the development and prognosis of diabetic cardiomyopathy (DCM). The anti-aging protein Klotho is suggested to modulate tissue inflammatory responses. The aim of the present study was to examine the protective effects of Klotho on DCM.Main methodsA streptozotocin-induced diabetes mouse model was established to assess the effects of Klotho in vivo, which was administered for 12 weeks. The characteristics of type 1 DCM were eva...
Source: Life Sciences - August 18, 2019 Category: Biology Source Type: research
Zinc supplementation alleviates OTA-induced oxidative stress and apoptosis in MDCK cells by up-regulating metallothioneins
Publication date: Available online 5 August 2019Source: Life SciencesAuthor(s): Hu Li, Rahmani Mohammad Malyar, Nianhui Zhai, Hong Wang, Kai Liu, Dandan Liu, Cuiling Pan, Fang Gan, Kehe Huang, Jinfeng Miao, Xingxiang ChenAbstractAimsThe present study was to investigate the protective effects of Zn supplementation in OTA-induced apoptosis of Madin-Darby canine kidney (MDCK) epithelial cells and explore the potential mechanisms. Aiming to provides a new insight into the treatment strategy of OTA-induced nephrotoxicity by nutritional regulation.Main methodsInitially, through MTT and LDH assay revealed that Zn supplementation ...
Source: Life Sciences - August 18, 2019 Category: Biology Source Type: research
LncRNA AK094457 promotes AngII-mediated hypertension and endothelial dysfunction through suppressing of activation of PPARγ
In conclusion, lncRNA AK094457 is identified as a key regulator in blood pressure and endothelial function, which can increase AngII-induced hypertension and endothelial dysfunction via suppression of PPARγ.
Source: Life Sciences - August 14, 2019 Category: Biology Source Type: research
Berberine induces ZIP14 expression and modulates zinc redistribution to protect intestinal mucosal barrier during polymicrobial sepsis
Publication date: 15 September 2019Source: Life Sciences, Volume 233Author(s): Yan He, Xiaoming Yuan, Hao Zuo, Xiangwei Li, Ying Sun, Aiwen FengAbstractAimsThe present study investigated if berberine might induce Zrt-Irt-like protein 14 (ZIP14) and affect zinc redistribution to protect intestinal barrier in sepsis.Main methodsRodent model of sepsis was induced by cecal ligation and puncture (CLP). Plasma endotoxin was assayed by LAL test and plasma zinc was measured by flame atomic spectrophotometer. Gut mucosal permeability was determined by plasma FITC-dextran. Zinc content and ZIP14 mRNA in gut mucosa were assayed by sp...
Source: Life Sciences - July 31, 2019 Category: Biology Source Type: research
The effects of Tel2 on cardiomyocyte survival
Publication date: 1 September 2019Source: Life Sciences, Volume 232Author(s): Naaiko Yorichika, Yuichi Baba, Briana K. Shimada, Manoj Thakore, Sharon M. Wong, Motoi Kobayashi, Jason K. Higa, Takashi MatsuiAbstractAimsOverexpression of the mechanistic target of rapamycin (mTOR), a member of the PIKK (phosphoinositide kinase-related kinase) family, protects cardiomyocytes from cell death induced by pathological stimuli such as ischemia. We previously reported that posttranslational modification of mTOR plays an important role in regulating cardiac mTOR expression. The aim of this study was to see if Tel2 (telomere maintenanc...
Source: Life Sciences - July 25, 2019 Category: Biology Source Type: research
MicroRNA-92b-3p suppresses angiotensin II-induced cardiomyocyte hypertrophy via targeting HAND2
In this study, we want to investigate the role of microRNA-92b-3p (miR-92b-3p) in cardiomyocyte hypertrophy and the mechanisms involved.Materials and methodsNeonatal mouse ventricular cells (NMVCs) were isolated from the hearts of 1–3-d-old newborn C57BL6 mice. The isolated NMVCs were induced hypertrophic phenotype by Angiotensin-II (Ang-II) and the cell size was examined by FITC-phalloidin staining assay. The expression of miR-92b-3p was determined by quantitative real-time PCR (qRT-qPCR). MRNA and protein level of β-MHC, ACTA1 and HAND2 in NMVCs transfected with miR-92b-3p mimic and inhibition were assessed by RT-qPCR...
Source: Life Sciences - July 6, 2019 Category: Biology Source Type: research
Calpain silencing alleviates myocardial ischemia-reperfusion injury through the NLRP3/ASC/Caspase-1 axis in mice
Publication date: Available online 3 July 2019Source: Life SciencesAuthor(s): Rong-Chuan Yue, Sheng-Zhong Lu, Yu Luo, Tao Wang, Hao Liang, Jing Zeng, Jie Liu, Hou-Xiang HuAbstractAimsPrior to reperfusion, Calpains remain inactive due to the acidic pH and elevated ionic strength in the ischemic myocardium; but Calpain is activated during myocardial reperfusion. The underlying mechanism of Calpain activation in the ischemia-reperfusion (I/R) is yet to be determined. Therefore, the present study aims to investigate the mechanism of Calpain in I/R-induced mice.Main methodsIn order to detect the function of Calpain and the NLRP...
Source: Life Sciences - July 4, 2019 Category: Biology Source Type: research
