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Source: Journal of Cellular and Molecular Medicine

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Total 94 results found since Jan 2013.

Cordycepin activates AMP‐activated protein kinase (AMPK) via interaction with the γ1 subunit
Abstract Cordycepin is a bioactive component of the fungus Cordyceps militaris. Previously, we showed that cordycepin can alleviate hyperlipidemia through enhancing the phosphorylation of AMP‐activated protein kinase (AMPK), but the mechanism of this stimulation is unknown. Here, we investigated the potential mechanisms of cordycepin‐induced AMPK activation in HepG2 cells. Treatment with cordycepin largely reduced oleic acid (OA)‐elicited intracellular lipid accumulation and increased AMPK activity in a dose‐dependent manner. Cordycepin‐induced AMPK activation was not accompanied by changes in either the intracel...
Source: Journal of Cellular and Molecular Medicine - November 28, 2013 Category: Molecular Biology Authors: Chongming Wu, Yanshen Guo, Yan Su, Xue Zhang, Hong Luan, Xiaopo Zhang, Huixin Zhu, Huixia He, Xiaoliang Wang, Guibo Sun, Xiaobo Sun, Peng Guo, Ping Zhu Tags: Original Article Source Type: research

Apelin inhibits the proliferation and migration of rat PASMCs via the activation of PI3K/Akt/mTOR signal and the inhibition of autophagy under hypoxia
This study provides the evidence that exogenous apelin treatment contributes to inhibit the proliferation and migration of PASMCs by regulating the level of autophagy.
Source: Journal of Cellular and Molecular Medicine - January 22, 2014 Category: Molecular Biology Authors: Hongyu Zhang, Yongsheng Gong, Zhouguang Wang, Liping Jiang, Ran Chen, Xiaofang Fan, Huanmian Zhu, Liping Han, Xiaokun Li, Jian Xiao, Xiaoxia Kong Tags: Original Article Source Type: research

CD34+VEGFR‐3+ progenitor cells have a potential to differentiate towards lymphatic endothelial cells
Abstract Endothelial progenitor cells (EPCs) play an important role in postnatal neovascularization. However, it is poorly understood whether EPCs contribute to lymphangiogenesis. Here, we assessed differentiation of a noval population of EPCs towards lymphatic endothelial cells and their lymphatic formation. CD34+VEGFR‐3+ EPCs were isolated from mononuclear cells of human cord blood by fluorescence‐activated cell sorting. These cells expressed CD133 and displayed the phenotype of the endothelial cells. Cell colonies appeared at 7–10 days after incubation. The cells of the colonies grew rapidly and could be repeated...
Source: Journal of Cellular and Molecular Medicine - January 22, 2014 Category: Molecular Biology Authors: Yu‐zhen Tan, Hai‐jie Wang, Mei‐hua Zhang, Zhe Quan, Ting Li, Qi‐zhi He Tags: Original Article Source Type: research

PMC, a potent hydrophilic α‐tocopherol derivative, inhibits NF‐κB activation via PP2A but not IκBα‐dependent signals in vascular smooth muscle cells
In this study, we investigated the mechanisms of the inhibitory effects of PMC in vascular smooth muscle cells (VSMCs) exposed to pro‐inflammatory stimuli, lipopolysaccharide (LPS) combined with interferon (IFN)‐γ. Treatment of LPS/IFN‐γ‐stimulated VSMCs with PMC suppressed the expression of inducible nitric oxide synthase (iNOS) and matrix metalloproteinase‐9 in a concentration‐dependent manner. A reduction in LPS/IFN‐γ‐induced nuclear factor (NF)‐κB activation was also observed in PMC‐treated VSMCs. The translocation and phosphorylation of p65, protein phosphatase 2A (PP2A) inactivation and the fo...
Source: Journal of Cellular and Molecular Medicine - April 13, 2014 Category: Molecular Biology Authors: Cheng‐Ying Hsieh, George Hsiao, Ming‐Jen Hsu, Yi‐Hsuan Wang, Joen‐Rong Sheu Tags: Original Article Source Type: research

Resveratrol‐enhanced autophagic flux ameliorates myocardial oxidative stress injury in diabetic mice
Abstract Autophagic dysfunction is observed in diabetes mellitus. Resveratrol has a beneficial effect on diabetic cardiomyopathy. Whether the resveratrol‐induced improvement in cardiac function in diabetes is via regulating autophagy remains unclear. We investigated the mechanisms underlying resveratrol‐mediated protection against heart failure in diabetic mice, with a focus on the role of sirtuin 1 (SIRT1) in regulating autophagic flux. Diabetic cardiomyopathy in mice was induced by streptozotocin (STZ). Long‐term resveratrol treatment improved cardiac function, ameliorated oxidative injury and reduced apoptosis in ...
Source: Journal of Cellular and Molecular Medicine - June 1, 2014 Category: Molecular Biology Authors: Bo Wang, Qing Yang, Yuan‐yuan Sun, Yi‐fan Xing, Ying‐bin Wang, Xiao‐ting Lu, Wen‐wu Bai, Xiao‐qiong Liu, Yu‐xia Zhao Tags: Original Article Source Type: research

Elongation factor‐2 kinase regulates TG2/β1 integrin/Src/uPAR pathway and epithelial–mesenchymal transition mediating pancreatic cancer cells invasion
Abstract Pancreatic ductal adenocarcinoma is one of the lethal cancers with extensive local tumour invasion, metastasis, early systemic dissemination and poorest prognosis. Thus, understanding the mechanisms regulating invasion/metastasis and epithelial–mesenchymal transition (EMT), is the key for developing effective therapeutic strategies for pancreatic cancer (PaCa). Eukaryotic elongation factor‐2 kinase (eEF‐2K) is an atypical kinase that we found to be highly up‐regulated in PaCa cells. However, its role in PaCa invasion/progression remains unknown. Here, we investigated the role of eEF‐2K in cellular invasi...
Source: Journal of Cellular and Molecular Medicine - September 12, 2014 Category: Molecular Biology Authors: Ahmed A. Ashour, Nilgun Gurbuz, Sultan Neslihan Alpay, Abdel‐Aziz H. Abdel‐Aziz, Ahmed M. Mansour, Longfei Huo, Bulent Ozpolat Tags: Original Article Source Type: research

KITLG is a novel target of miR‐34c that is associated with the inhibition of growth and invasion in colorectal cancer cells
In conclusion, our results demonstrated that miR‐34c might interfere with KITLG‐related CRC and could be a novel molecular target for CRC patients.
Source: Journal of Cellular and Molecular Medicine - September 12, 2014 Category: Molecular Biology Authors: Shu Yang, Wen‐shuai Li, Fang Dong, Hai‐mei Sun, Bo Wu, Jun Tan, Wan‐jing Zou, De‐shan Zhou Tags: Original Article Source Type: research

ASPP2 attenuates triglycerides to protect against hepatocyte injury by reducing autophagy in a cell and mouse model of non‐alcoholic fatty liver disease
In conclusion, ASPP2 may participate in the lipid metabolism of non‐alcoholic steatohepatitis and attenuate liver failure.
Source: Journal of Cellular and Molecular Medicine - September 25, 2014 Category: Molecular Biology Authors: Fang Xie, Lin Jia, Minghua Lin, Ying Shi, Jiming Yin, Yin Liu, Dexi Chen, Qinghua Meng Tags: Original Article Source Type: research

PDGF beta targeting in cervical cancer cells suggest a fine‐tuning of compensatory signalling pathways to sustain tumourigenic stimulation
In this study, we used siRNA against PDGF beta (PDGFBB) to investigate the cellular and molecular mechanisms of PDGFBB signalling in Ca Ski and HeLa cervical cancer cells. Our results show that PDGFBB inhibition in Ca Ski cells led to rapid alterations of the transcriptional pattern of 579 genes, genes that are known to have antagonistic roles in regulating tumour progression. Concomitantly, with the lack of significant effects on cervical cancer cells proliferation, apoptosis, migration or invasion, these findings suggests that cervical cancer cells shift between compensatory signalling pathways to maintain their behaviou...
Source: Journal of Cellular and Molecular Medicine - October 14, 2014 Category: Molecular Biology Authors: Oana Mihaela Tudoran, Olga Soritau, Loredana Balacescu, Laura Pop, Guillaume Meurice, Simona Visan, Staffan Lindberg, Alexandru Eniu, Ulo Langel, Ovidiu Balacescu, Ioana Berindan‐Neagoe Tags: Original Article Source Type: research

HDAC‐inhibitor (S)‐8 disrupts HDAC6‐PP1 complex prompting A375 melanoma cell growth arrest and apoptosis
Abstract Histone deacetylase inhibitors (HDACi) are agents capable of inducing growth arrest and apoptosis in different tumour cell types. Previously, we reported a series of novel HDACi obtained by hybridizing SAHA or oxamflatin with 1,4‐benzodiazepines. Some of these hybrids proved effective against haematological and solid cancer cells and, above all, compound (S)‐8 has emerged for its activities in various biological systems. Here, we describe the effectiveness of (S)‐8 against highly metastatic human A375 melanoma cells by using normal PIG1 melanocytes as control. (S)‐8 prompted: acetylation of histones H3/H4 ...
Source: Journal of Cellular and Molecular Medicine - November 1, 2014 Category: Molecular Biology Authors: Manjola Balliu, Luca Guandalini, Maria Novella Romanelli, Massimo D'Amico, Francesco Paoletti Tags: Original Article Source Type: research

Connexin 31.1 degradation requires the Clathrin‐mediated autophagy in NSCLC cell H1299
In this study, Cx31.1 was suggested to be degraded through both ubiquitin–proteasome system (UPS) and autophagy. Blockage of UPS with MG‐132 increased Cx31.1 level, but could not inhibit the degradation of Cx31.1 completely. In H1299 cells stably expressing Cx31.1, Cx31.1 reduced when autophagy was induced through starvation or Brefeldin A treatment. Knockdown of autophagy‐related protein ATG5 could increase the cellular level of Cx31.1 both under normal growth condition and starvation‐induced autophagy. Colocalization of Cx31.1 and autophagy marker light chain 3 (LC3) was revealed by immunofluorescence analysis. C...
Source: Journal of Cellular and Molecular Medicine - November 11, 2014 Category: Molecular Biology Authors: Xingli Zhu, Zhenchao Ruan, Xiufang Yang, Kaili Chu, Hai Wu, Yao Li, Yan Huang Tags: Original Article Source Type: research

Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin‐resistant uterine cancer
In this study, low‐abundant mitochondrial proteins were enriched for proteomic analysis with the state‐of‐the‐art two‐dimensional differential gel electrophoresis (2D‐DIGE) and matrix‐assistant laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) strategy to compare and identify the mitochondrial protein profiling changes in response to the development of doxorubicin resistance in human uterine cancer cells. The mitochondrial proteomic results demonstrate more than fifteen hundred protein features were resolved from the equal amount pooled of three purified mitochondrial proteins ...
Source: Journal of Cellular and Molecular Medicine - January 30, 2015 Category: Molecular Biology Authors: Yi‐Wen Lo, Szu‐Ting Lin, Shing‐Jyh Chang, Chia‐Hao Chan, Kevin W. Lyu, Jo‐Fan Chang, Eugenie Wong Soon May, Dai‐Ying Lin, Hsiu‐Chuan Chou, Hong‐Lin Chan Tags: Original Article Source Type: research

Overexpression of deubiquitinating enzyme USP28 promoted non‐small cell lung cancer growth
Abstract Non‐small cell lung cancer (NSCLC) accounts for most lung cancer. To develop new therapy required the elucidation of NSCLC pathogenesis. The deubiquitinating enzymes USP 28 has been identified and studied in colon and breast carcinomas. However, the role of USP28 in NSCLC is unknown. The level mRNA or protein level of USP28 were measured by qRT‐PCR or immunohistochemistry (IHC). The role of USP28 in patient survival was revealed by Kaplan–Meier plot of overall survival in NSCLC patients. USP28 was up or down regulated by overexpression plasmid or siRNA transfection. Cell proliferation and apoptosis was assay...
Source: Journal of Cellular and Molecular Medicine - February 5, 2015 Category: Molecular Biology Authors: Lei Zhang, Biao Xu, Yong Qiang, Hairong Huang, Changtian Wang, Demin Li, Jianjun Qian Tags: Original Article Source Type: research

Lin28a protects against cardiac ischaemia/reperfusion injury in diabetic mice through the insulin‐PI3K‐mTOR pathway
This study indicates that lin28a overexpression reduces IS, improves cardiac function, decreases cardiomyocyte apoptosis index and alleviates cardiomyocyte mitochondria impairment after cardiac I/R injury in diabetic mice. The mechanism responsible for the effects of lin28a is associated with the insulin‐PI3K‐mTOR dependent pathway.
Source: Journal of Cellular and Molecular Medicine - February 16, 2015 Category: Molecular Biology Authors: Mingming Zhang, Dongdong Sun, Shuang Li, Xietian Pan, Xiaotian Zhang, Di Zhu, Congye Li, Rongqing Zhang, Erhe Gao, Haichang Wang Tags: Original Article Source Type: research

TLR3/TRIF signalling pathway regulates IL‐32 and IFN‐β secretion through activation of RIP‐1 and TRAF in the human cornea
In this study, we investigated how the TLR3 and RIG‐I signalling pathway was stimulated by viral infection to produce interleukin (IL)‐32‐mediated pro‐inflammatory cytokines and type I interferon in the corneal epithelium using Epstein–Barr virus (EBV)‐infected human cornea epithelial cells (HCECs/EBV) as a model of viral keratitis. Increased TLR3 and RIG‐I that are responded to EBV‐encoded RNA 1 and 2 (EBER1 and EBER2) induced the secretion of IL‐32‐mediated pro‐inflammatory cytokines and IFN‐β through up‐regulation of TRIF/TRAF family proteins or RIP‐1. TRIF silencing or TLR3 inhibitors more ...
Source: Journal of Cellular and Molecular Medicine - March 6, 2015 Category: Molecular Biology Authors: Ga Bin Park, Dae Young Hur, Yeong Seok Kim, Hyun‐Kyung Lee, Jae Wook Yang, Daejin Kim Tags: Original Article Source Type: research