• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

siRNA distribution and pharmacokinetics assessed by nuclear imaging are modulated according to radiolabelling site
This study aimed at defining the biodistribution and pharmacokinetics properties of a siRNA directed to the Casein Kinase-2 beta (CK2β) subunit, a potential target in cancer therapy.
Source: Nuclear Medicine and Biology - April 24, 2015 Category: Nuclear Medicine Authors: Dominique Garin, Mitra Ahmadi, Anne-Sophie Gauchez, Sylvain Bohic, Sandra Boccard, Peter Cloetens, Daniel Fagret, François Berger, Arnaud Briat, Laurent Pelletier, Catherine Ghezzi Source Type: research

Demonstration of dose dependent cytotoxic activity in SW480 colon cancer cells by 177Lu-labeled siRNA targeting IGF-1R
In this study, radioconjugate of IGF-1R siRNA with lutetium-177 (177Lu) was produced, and the anti-proliferation and apoptosis effects elicited by this 177Lu-siRNA complex in the SW480 colon cancer cells were evaluated.Methods: IGF-1R and Luciferase siRNAs were conjugated with p-SCN-Bn-DTPA, and then radiolabeled with 177Lu. The effects of labeled and non-labeled IGF-1R siRNAs on IGF-1R expression were assessed with RT-PCR analysis and ELISA assay. IGF-1R siRNAs induced cell death and apoptosis were investigated using MTT assay and Annexin-V/propidium iodide (PI) double staining, respectively.Results: Combined purification...
Source: Nuclear Medicine and Biology - April 27, 2013 Category: Radiology Authors: Mojtaba Fathi, Mohammad Taghikhani, Mohammad Ghannadi-Maragheh, Kamal Yavari Source Type: research