Filtered By:
Source: Molecular and Cellular Proteomics : MCP

This page shows you your search results in order of date.

Order by Relevance | Date

Total 28 results found since Jan 2013.

eEF2K activity determines synergy to co-treatment of cancer cells with PI3K and MEK inhibitors
Mol Cell Proteomics. 2022 May 2:100240. doi: 10.1016/j.mcpro.2022.100240. Online ahead of print.ABSTRACTPI3K-mTOR and MEK/MAPK are the most frequently dysregulated signaling pathways in cancer. A problem that limits the success of therapies that target individual PI3K-MAPK members is that these pathways converge to regulate downstream functions and often compensate each other, leading to drug resistance and transient responses to therapy. In order to overcome resistance, therapies based on co-treatments with PI3K/AKT and MEK/MAPK inhibitors are now being investigated in clinical trials but the mechanisms of sensitivity to ...
Source: Molecular and Cellular Proteomics : MCP - May 5, 2022 Category: Molecular Biology Authors: Maruan Hijazi Pedro Casado Nosheen Akhtar Saul Alvarez-Teijeiro Vinothini Rajeeve Pedro R Cutillas Source Type: research

Characterization of the secretome, transcriptome and proteome of human β cell line EndoC-βH1
Mol Cell Proteomics. 2022 Apr 1:100229. doi: 10.1016/j.mcpro.2022.100229. Online ahead of print.ABSTRACTEarly diabetes research is hampered by limited availability, variable quality and instability of human pancreatic islets in culture. Little is known about the human β cell secretome, and recent studies question translatability of rodent β cell secretory profiles. Here, we verify representativeness of EndoC-βH1, one of the most widely used human β cell lines, as a translational human β cell model based on omics and characterize the EndoC-βH1 secretome. We profiled EndoC-βH1 cells using RNA-seq, Data Independent Acq...
Source: Molecular and Cellular Proteomics : MCP - April 4, 2022 Category: Molecular Biology Authors: Maria Ryaboshapkina Kevin Saitoski Ghaith M Hamza Andrew F Jarnuczak S éverine Pechberty Claire Berthault Kaushik Sengupta Christina Rye Underwood Shalini Andersson Raphael Scharfmann Source Type: research

Glutamine-fructose-6-phosphate transaminase 2 (GFPT2) is upregulated in breast epithelial-mesenchymal transition and responds to oxidative stress
Mol Cell Proteomics. 2021 Dec 16:100185. doi: 10.1016/j.mcpro.2021.100185. Online ahead of print.ABSTRACTBreast cancer cells that have undergone partial epithelial-mesenchymal transition (EMT) are believed to be more invasive than cells that have completed EMT. To study metabolic reprogramming in different mesenchymal states, we analyzed protein expression following EMT in the breast epithelial cell model D492 with single-shot LFQ supported by a SILAC proteomics approach. The D492 EMT cell model contains three cell lines: the epithelial D492 cells, the mesenchymal D492M cells, and a partial mesenchymal, tumorigenic variant...
Source: Molecular and Cellular Proteomics : MCP - December 19, 2021 Category: Molecular Biology Authors: Qiong Wang Sigurdur Trausti Karvelsson Aristotelis Kotronoulas Thorarinn Gudjonsson Skarphedinn Halldorsson Ottar Rolfsson Source Type: research

Quantitative proteomics links the LRRC59 interactome to mRNA translation on the ER membrane.
Abstract Protein synthesis on the endoplasmic reticulum (ER) requires the dynamic coordination of numerous cellular components. Together, resident ER membrane proteins, cytoplasmic translation factors, and both integral membrane and cytosolic RNA-binding proteins operate in concert with membrane-associated ribosomes to facilitate ER-localized translation. Little is known, however, regarding the spatial organization of ER-localized translation. This question is of growing significance as it is now known that ER-bound ribosomes contribute to secretory, integral membrane, and cytosolic protein synthesis alike. To exp...
Source: Molecular and Cellular Proteomics : MCP - August 10, 2020 Category: Molecular Biology Authors: Hannigan MM, Hoffman AM, Thompson W, Zheng T, Nicchitta CV Tags: Mol Cell Proteomics Source Type: research

Immediate adaptation analysis implicates BCL6 as an EGFR-TKI combination therapy target in NSCLC.
In conclusion, or data indicates that multiple different adaptive mechanisms may act in concert to blunt the cellular impact of EGFR inhibition, and we suggest BCL6 as a potential target for EGFR inhibitor-based combination therapy. PMID: 32234966 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Proteomics : MCP - March 30, 2020 Category: Molecular Biology Authors: Zhou Tran Y, Minozada R, Cao X, Johansson HJ, Branca RMM, Seashore-Ludlow B, Orre LM Tags: Mol Cell Proteomics Source Type: research

Comparative proteomics of dying and surviving cancer cells improves  the identification of drug targets and sheds light on cell life/death decisions.
Comparative proteomics of dying and surviving cancer cells improves the identification of drug targets and sheds light on cell life/death decisions. Mol Cell Proteomics. 2018 Mar 23;: Authors: Saei AA, Sabatier P, Güler Tokat Ü, Chernobrovkin A, Pirmoradian M, Zubarev RA Abstract Chemotherapeutics cause the detachment and death of adherent cancer cells. When studying the proteome changes to determine the protein target and mechanism of action of anticancer drugs, the still-attached cells are normally used, while the detached cells are usually ignored. To test the hypothesis that proteomes of detach...
Source: Molecular and Cellular Proteomics : MCP - March 23, 2018 Category: Molecular Biology Authors: Saei AA, Sabatier P, Güler Tokat Ü, Chernobrovkin A, Pirmoradian M, Zubarev RA Tags: Mol Cell Proteomics Source Type: research

Identification of differentially expressed splice variants by the proteogenomic pipeline Splicify.
Abstract Proteogenomics, i.e. comprehensive integration of genomics and proteomics data, is a powerful approach identifying novel protein biomarkers. This is especially the case for proteins that differ structurally between disease and control conditions. As tumor development is associated with aberrant splicing, we focus on this rich source of cancer specific biomarkers. To this end, we developed a proteogenomic pipeline, Splicify, which is able to detect differentially expressed protein isoforms. Splicify is based on integrating RNA massive parallel sequencing data and tandem mass spectrometry proteomics data to...
Source: Molecular and Cellular Proteomics : MCP - July 26, 2017 Category: Molecular Biology Authors: Komor MA, Pham T, Hiemstra AC, Piersma SR, Bolijn AS, Schelfhorst T, Delis-van Diemen PM, Tijssen M, Sebra RP, Ashby M, Meijer GA, Jimenez CR, Fijneman RJA Tags: Mol Cell Proteomics Source Type: research

Dynamics of protein expression reveals primary targets and secondary messengers of estrogen receptor alpha signaling in MCF-7 breast cancer cells.
Abstract Estrogen receptor alpha (ERα)-mediated proliferation of breast cancer cells is facilitated through expression of multiple primary target genes, products of which induce a secondary response to stimulation. To differentiate between the primary and secondary target proteins of ERα signaling, we measured dynamics of protein expression induced by 17β-estradiol in MCF-7 breast cancer cells. Measurement of the global proteomic effects of estradiol by stable isotope labeling by amino acids in cell culture (SILAC) resulted in identification of 103 estrogen-regulated proteins, with only 40 of the corresponding ...
Source: Molecular and Cellular Proteomics : MCP - April 10, 2016 Category: Molecular Biology Authors: Drabovich AP, Pavlou MP, Schiza C, Diamandis EP Tags: Mol Cell Proteomics Source Type: research

NADH-cytochrome b5 reductase 3 promotes colonization and metastasis formation and is a prognostic marker of disease-free and overall survival in estrogen receptor-negative breast cancer.
Abstract Metastasis is the main cause of cancer-related deaths and remains the most significant challenge to management of the disease. Metastases are established through a complex multistep process involving intracellular signaling pathways. To gain insight to proteins central to specific steps in metastasis formation, we used a metastasis cell line model that allows investigation of extravasation and colonization of circulating cancer cells to lungs in mice. Using stable isotopic labeling by amino acids in cell culture and subcellular fractionation, the nuclear, cytosol and mitochondria proteomes were analyzed b...
Source: Molecular and Cellular Proteomics : MCP - September 8, 2015 Category: Molecular Biology Authors: Lund RR, Leth-Larsen R, Caterino TD, Terp MG, Nissen J, Lænkholm AV, Jensen ON, Ditzel HJ Tags: Mol Cell Proteomics Source Type: research