• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Efficient in vitro gene therapy with PEG siRNA lipid nanocapsules for passive targeting strategy in melanoma.
The objective of this work consists in formulating and optimizing the encapsulation of siRNA into lipid nanocapsules (LNCs) for efficient gene therapy on melanoma cells. SiRNA LNCs were prepared from DOTAP/DOPE lipoplexes, and the siRNA dose and lipid/siRNA charge ratio were assayed to improve the stability and encapsulation yield. Cryo-TEM imaging of the siRNA lipoplexes and LNC morphology revealed a specific organization of the siRNA DOTAP/DOPE lipoplexes as well as specific lipid microstructures. No cytotoxicity of the siRNA LNCs against the melanoma SK-Mel28 cell line was observed at concentrations of up to 500 ng/mL s...
Source: Biotechnology Journal - September 26, 2014 Category: Biotechnology Authors: Resnier P, LeQuinio P, Lautram N, André E, Gaillard C, Bastiat G, Benoit JP, Passirani C Tags: Biotechnol J Source Type: research

Study and Evaluation of the Potential of Lipid Nanocarriers for Transdermal Delivery of siRNA.
In this study, we used 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or lipofectamine to prepare a nanocarrier for delivering siRNA against glyceraldehyde 3-phosphate dehydrogenase (GAPDH); GAPDH expression was then evaluated at the cellular level. In addition, a dermal transport assay was designed and implemented to evaluate the penetration and delivery efficacy of siRNA in pig skin using lipid nanocarriers. The delivery of siRNA with the use of a lipid nanocarrier was significantly better than the delivery of siRNA without it. Thus, Our findings identify lipid nanocarriers as excellent candidates for the transdermal d...
Source: Biotechnology Journal - July 16, 2020 Category: Biotechnology Authors: Lee K, Min D, Choi Y, Kim J, Yoon S, Jang J, Park S, Tanaka M, Cho YW, Koo HJ, Jeon H, Choi J Tags: Biotechnol J Source Type: research

Identifying HIPK1 as target of miR-22-3p enhancing recombinant protein production from HEK 293 cell by using microarray and HTP siRNA screen.
Abstract Protein expression from human embryonic kidney cells (HEK 293) is an important tool for structural and clinical studies. It was previously shown that microRNAs (small, noncoding RNAs) are effective means for improved protein expression from these cells, and by conducting a high-throughput screening of the human microRNA library, several microRNAs were identified as potential candidates for improving expression. From these, miR-22-3p was chosen for further study since it increased the expression of luciferase, two membrane proteins and a secreted fusion protein with minimal effect on the cells' growth and ...
Source: Biotechnology Journal - October 7, 2017 Category: Biotechnology Authors: Inwood S, Buehler E, Betenbaugh M, Lal M, Shiloach J Tags: Biotechnol J Source Type: research

Analysis of polymeric nanoparticle properties for siRNA/DNA delivery in a tumor xenograft tissue slice air-liquid interface model
CONCLUSION: The ex vivo 3D tissue slice ALI culture model is a powerful system that allows the effective evaluation of biological nanoparticle efficacy and biocompatibility in an intact tissue environment. It is comparably inexpensive, time-saving, and follows the 3R principle, while allowing the identification of critical nanoparticle properties and optimal candidates for in vivo applications. This article is protected by copyright. All rights reserved.PMID:36541426 | DOI:10.1002/biot.202200415
Source: Biotechnology Journal - December 21, 2022 Category: Biotechnology Authors: Michael Karimov Marlene Scherer Heike Franke Alexander Ewe Achim Aigner Source Type: research

Pages: 1  2  3  4