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Research Progress of nucleic acid delivery vectors for gene therapy.
Abstract Gene therapy has broad prospects as an effective treatment for some cancers and hereditary diseases. However, DNA and siRNA are easily degraded in vivo because of their biological activities as macromolecules, and they need the effective transmembrane delivery carrier Selecting the appropriate carrier for delivery will allow nucleic acid molecules to reach their site of action and enhance delivery efficiency. Currently used nucleic acid delivery vectors can be divided into two major categories: viral and non-viral vectors. Viral carrier transport efficiency is high, but there are safety issues. Non-viral ...
Source: Biomedical Microdevices - January 26, 2020 Category: Biomedical Engineering Authors: Jiao Y, Xia ZL, Ze LJ, Jing H, Xin B, Fu S Tags: Biomed Microdevices Source Type: research

Micelle-like nanoparticles as siRNA and miRNA carriers for cancer therapy.
Abstract Gene therapy has emerged as an alternative in the treatment of cancer, particularly in cases of resistance to chemo and radiotherapy. Different approaches to deliver genetic material to tumor tissues have been proposed, including the use of small non-coding RNAs due to their multiple mechanisms of action. However, such promise has shown limits in in vivo application related to RNA's biological instability and stimulation of immunity, urging the development of systems able to overcome those barriers. In this review, we discuss the use of RNA interference in cancer therapy with special attention to the role...
Source: Biomedical Microdevices - July 12, 2018 Category: Biomedical Engineering Authors: Costa DF, Torchilin VP Tags: Biomed Microdevices Source Type: research

Microfabricated infuse-withdraw micropump component for an integrated inner-ear drug-delivery platform.
Abstract One of the major challenges in treatment of auditory disorders is that many therapeutic compounds are toxic when delivered systemically. Local intracochlear delivery methods are becoming critical in emerging treatments and in drug discovery. Direct infusion via cochleostomy, in particular, is attractive from a pharmacokinetics standpoint, as there is potential for the kinetics of delivery to be well-controlled. Direct infusion is compatible with a large number of drug types, including large, complex molecules such as proteins and unstable molecules such as siRNA. In addition, hair-cell regeneration therap...
Source: Biomedical Microdevices - February 20, 2015 Category: Biomedical Engineering Authors: Tandon V, Kang WS, Spencer AJ, Kim ES, Pararas EE, McKenna MJ, Kujawa SG, Mescher MJ, Fiering J, Sewell WF, Borenstein JT Tags: Biomed Microdevices Source Type: research

Detection of GNAQ mutations and reduction of cell viability in uveal melanoma cells with functionalized gold nanoparticles.
CONCLUSION: AuNPs may in future be developed to serve as sensors for mutations of vital importance. The new release system for siRNA-AuNP improves previous systems, which conceivably will be useful for future therapeutic gene regulatory approaches. PMID: 25653058 [PubMed - in process]
Source: Biomedical Microdevices - February 1, 2015 Category: Biomedical Engineering Authors: Posch C, Latorre A, Crosby MB, Celli A, Latorre A, Vujic I, Sanlorenzo M, Green GA, Weier J, Zekhtser M, Ma J, Monico G, Char DH, Jusufbegovic D, Rappersberger K, Somoza Á, Ortiz-Urda S Tags: Biomed Microdevices Source Type: research

A new spiral microelectrode assembly for electroporation and impedance measurements of adherent cell monolayers.
In this study, a new microelectrode assembly based on spiral geometry applicable to in situ electroporation of adherent cell monolayers on standard multiwell plates is presented. Furthermore, the structure is specially conceived to perform electrical impedance spectroscopy (EIS) measurements during electroporation. Its performance for cell membrane permeabilization is tested with a fluorescent probe. Gene electrotransfer is also assayed using a plasmid DNA encoding GFP in four different cell lines (CHO, HEK293, 3T3-L1 and FTO2B). Additionally, siRNA α-GFP electrotransfection is tested in GFP gene-expressing CHO cells. Our...
Source: Biomedical Microdevices - March 30, 2014 Category: Biomedical Engineering Authors: García-Sánchez T, Guitart M, Rosell-Ferrer J, Gómez-Foix AM, Bragós R Tags: Biomed Microdevices Source Type: research