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Source: Biomacromolecules

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Total 92 results found since Jan 2013.

Aggregation Behavior of Cationic Nanohydrogel Particles in Human Blood Serum.
Abstract For systemic siRNA delivery applications, well-defined drug carriers are required that guarantee stability for both carrier and cargo. Among various concepts progressing in market or final development, cationic nanohydrogel particles may serve as novel transport media especially designed for siRNA-in vivo experiments. In this work, the interaction of nanohydrogel particles with proteins and serum components was studied via dynamic light scattering in human blood serum as novel screening method prior to applications in vivo. The formation of larger aggregates mostly caused by charge interaction with albumi...
Source: Biomacromolecules - April 3, 2014 Category: Biochemistry Authors: Nuhn L, Gietzen S, Mohr K, Fischer K, Toh K, Miyata K, Matsumoto Y, Kataoka K, Schmidt M, Zentel R Tags: Biomacromolecules Source Type: research

Redox-Sensitive PEG-Polypeptide Nanoporous Particles for Survivin Silencing in Prostate Cancer Cells.
We report the engineering of intracellular redox-responsive nanoporous poly(ethylene glycol)-poly(l-lysine) particles (NPEG-PLLs). The obtained particles exhibit no toxicity while maintaining the capability to deliver a small interfering RNA sequence (siRNA) targeting the anti-apoptotic factor, survivin, in prostate cancer cells. The redox-mediated cleavage of the disulfide bonds stabilizing the NPEG-PLL-siRNA complex results in the release of bioactive siRNA into the cytosol of prostate cancer PC-3 cells, which, in turn, leads to the effective silencing (∼59 ± 8%) of the target gene. These findings, obtained under opti...
Source: Biomacromolecules - June 29, 2015 Category: Biochemistry Authors: Cavalieri F, Beretta GL, Cui J, Braunger JA, Yan Y, Richardson JJ, Tinelli S, Folini M, Zaffaroni N, Caruso F Tags: Biomacromolecules Source Type: research

Development of Fully Degradable Phosphonium-Functionalized Amphiphilic Diblock Copolymers for Nucleic Acids Delivery.
Abstract To expand the range of functional polymer materials to include fully hydrolytically degradable systems that bear bioinspired phosphorus-containing linkages both along the backbone and as cationic side chain moieties for packaging and delivery of nucleic acids, phosphonium-functionalized polyphosphoester- block-poly(l-lactide) copolymers of various compositions were synthesized, fully characterized, and their self-assembly into nanoparticles were studied. First, an alkyne-functionalized polyphosphoester- block-poly(l-lactide) copolymer was synthesized via a one pot sequential ring opening polymerization of...
Source: Biomacromolecules - March 11, 2018 Category: Biochemistry Authors: Borguet YP, Khan S, Noel A, Gunsten SP, Brody SL, Elsabahy M, Wooley KL Tags: Biomacromolecules Source Type: research

Smurf1 knocked-down, mesenchymal stem cells and BMP-2 in an electrospun system for bone regeneration.
Abstract A sandwich-like system, fabricated with electrospun, poly (lactic-co-glycolic-acid) (PLGA) membranes incorporating either human recombinant bone morphogenetic protein 2 (BMP-2) enriched microspheres, rat bone marrow mesenchymal stem cells (rMSC), or rMSC with their Smurf1 (Smad Ubiquitin Regulatory Factor-1) expression knocked down by means of siRNA (rMSC573) at varying densities was evaluated in a rat calvarial, critical-size defect. The behavior of four membrane varieties, fabricated with different PLGA copolymers, was initially studied in rMSC cultures to decide on optimal membrane degradation and cell...
Source: Biomacromolecules - February 22, 2014 Category: Biochemistry Authors: Rodriguez-Evora M, García-Pizarro E, Del Rosario C, Perez-Lopez J, Reyes R, Delgado A, Rodriguez-Rey JC, Evora C Tags: Biomacromolecules Source Type: research

Michael Addition Polymerization of Trifunctional Amine and Acrylic Monomer: A Versatile Platform for Development of Biomaterials.
Abstract Michael addition polymerizations of amines and acrylic monomers are versatile approaches to biomaterials for various applications. A combinatorial library of poly(β-amino ester)s and diverse poly(amido amine)s from diamines and diacrylates or bisacrylamides have been reported, respectively. Furthermore novel linear and hyperbranched polymers from Michael addition polymerizations of trifunctional amines and acrylic monomers significantly enrich this category of biomaterials. In this Review, we focus on the biomaterials from Michael addition polymerizations of trifunctional amines and acrylic monomers. Fir...
Source: Biomacromolecules - September 5, 2016 Category: Biochemistry Authors: Cheng W, Wu DC, Liu Y Tags: Biomacromolecules Source Type: research

Synthesis and in vitro evaluation of monodisperse amino-functional polyester dendrimers with rapid degradability and antibacterial properties.
Abstract Amine functional polymers, especially cationically charged, are interesting biomacromolecules for several reasons including easy cell membrane entrance, their ability to escape endosomes through the proton sponge effect, spontaneous complexation and delivery of drugs and siRNA and simple functionalization in aqueous solutions. Dendrimers, a subclass of precision polymers, are monodisperse and exhibit a large and exact number of peripheral end groups in relation to their size and have shown promise in drug delivery, biomedical imaging and as anti-viral agents. In this work, hydroxyl functional dendrimers o...
Source: Biomacromolecules - November 13, 2017 Category: Biochemistry Authors: Stenström P, Hjort E, Zhang Y, Andrén OCJ, Guette-Marquet S, Schultzberg M, Malkoch M Tags: Biomacromolecules Source Type: research

Tunability of Biodegradable Poly(amine-co-ester) Polymers for Customized Nucleic Acid Delivery and Other Biomedical Applications.
Abstract Gene therapy promises to treat diseases that arise from genetic abnormalities by correcting the underlying cause of the disease rather than treating the associated symptoms. Successful transfer of nucleic acids into cells requires efficient delivery vehicles that protect the cargo and can penetrate the appropriate cellular barriers before releasing their contents. Many viral vectors and synthetic polycationic vectors for nucleic acid delivery do not translate well from in vitro to in vivo applications due to their instability and toxicity. We synthesized and characterized a library of biocompatible low ch...
Source: Biomacromolecules - August 15, 2018 Category: Biochemistry Authors: Kauffman AC, Piotrowski-Daspit AS, Nakazawa KH, Jiang Y, Datye A, Saltzman WM Tags: Biomacromolecules Source Type: research

Acid degradable cationic galactose-based hyperbranched polymers as nano-therapeutic vehicles for Epidermal Growth Factor Receptor (EGFR) knockdown in Cervical Carcinoma.
In this study we synthetized by reversible addition-fragmentation chain transfer (RAFT) polymerization, several acid degradable galactose-based hyperbranched cationic polymers with varying molecular weights (10 to 20 kDa) and compositions with 2-lactobioamidoethyl methacrylamide [LAEMA] and 2- aminoethyl methacrylamide hydrochloride [AEMA] at different ratios (2.0, 1.0 & 0.5) and evaluated their ability to enhance Epidermal Growth Factor Receptor (EGFR) knockdown in cervical carcinoma. All the polymer constructs have enhanced capabilities to condensate siRNA (short interfering RNA), showing low toxicity at higher LAEMA...
Source: Biomacromolecules - August 29, 2018 Category: Biochemistry Authors: Peng YY, Diaz-Dussan D, Kumar P, Narain R Tags: Biomacromolecules Source Type: research

A Kinetic Model of Oligonucleotide-Brush Interactions for the Rational Design of Gene Delivery Vectors.
Abstract Polymer brushes are attractive candidates for the design of gene delivery vectors as they allow the systematic study of the impact of structural (type, size and shape of nanomaterials core) and physico-chemical parameters (cationic monomer chemistry, brush thickness and grafting density) on transfection efficiency. However, relatively little is known of their interactions of oligonucleotides. To study such interactions, we use surface plasmon resonance and developed a kinetic model of brush binding and infiltration. We identify the striking impact that brush grafting density and thickness have on oligonuc...
Source: Biomacromolecules - April 23, 2019 Category: Biochemistry Authors: Qu F, Li D, Ma X, Chen F, Gautrot J Tags: Biomacromolecules Source Type: research

Polymeric Carriers for Nucleic Acid Delivery: Current Designs and Future Directions.
Abstract Within the last two decades a series of novel therapeutic nucleic acids entered research and clinical evaluation. Their differences both in biophysical properties as well as in mode and site of biological action provide polymer-based carriers with new delivery challenges. Recent tailor-made designs of polymeric carriers are reviewed that were optimized for nucleic acid cargos such as plasmid DNA, siRNA and micro RNA, messenger RNA or genome-modifying nucleic acids. The specific requirements for the various therapeutic cargos are discussed. Future directions include dynamic bioresponsive polymers as compon...
Source: Biomacromolecules - September 8, 2019 Category: Biochemistry Authors: Peng L, Wagner E Tags: Biomacromolecules Source Type: research

Tackling the Limitations of Copolymeric Small Interfering RNA Delivery Agents by a Combined Experimental-Computational Approach.
In this study, we tackle the drawbacks of the existing systems by a combined experimental-computational in-depth investigation of the influence of the polymer architecture over the binding and transfection efficiency. For that purpose, a library of diblock copolymers with a molar mass of 30 kDa and a narrow dispersity (Đ < 1.12) was synthesized. We studied in detail the impact of an altered block size and/or composition of cationic diblock copolymers on the viability of each respective structure as a delivery agent for polynucleotides. The experimental investigation was further complemented by a computational study emp...
Source: Biomacromolecules - November 3, 2019 Category: Biochemistry Authors: Tabujew I, Heidari M, Freidel C, Helm M, Tebbe L, Wolfrum U, Nagel-Wolfrum K, Koynov K, Biehl P, Schacher FH, Potestio R, Peneva K Tags: Biomacromolecules Source Type: research

Pluronic Micelle-Mediated Tissue Factor Silencing Enhances Hemocompatibility, Stemness, Differentiation Potential, and Paracrine Signaling of Mesenchymal Stem Cells
Biomacromolecules. 2021 Apr 5. doi: 10.1021/acs.biomac.1c00070. Online ahead of print.ABSTRACTMesenchymal stem/stromal cells (MSCs) evoke great excitement for treating different human diseases due to their ability to home inflamed tissues, suppress inflammation, and promote tissue regeneration. Despite great promises, clinical trial results are disappointing as allotransplantation of MSCs trigger thrombotic activity and are damaged by the complement system, compromising their survival and function. To overcome this, a new strategy is presented by the silencing of tissue factor (TF), a transmembrane protein that mediates pr...
Source: Biomacromolecules - April 5, 2021 Category: Biochemistry Authors: Vignesh K Rangasami Ganesh Nawale Kenta Asawa Sandeep Kadekar Sumanta Samanta Bo Nilsson Kristina N Ekdahl Susanna Miettinen J öns Hilborn Yuji Teramura Oommen P Varghese Oommen P Oommen Source Type: research

Genetic Engineering of Dendritic Cells Using Partially Zwitterionic Dendrimer-Entrapped Gold Nanoparticles Boosts Efficient Tumor Immunotherapy
Biomacromolecules. 2022 Mar 2. doi: 10.1021/acs.biomac.1c01571. Online ahead of print.ABSTRACTEffective processing and cross-priming of tumor neoantigen by dendritic cells (DCs) to T cells for spontaneous immune response generation to effectively kill cancer cells remain challenging in cancer immunotherapy. Here, we report a general approach to genetically engineer DCs through silencing their YTHDF1 protein (an important reader protein responsible for RNA m6A methylation) expression via a dendrimeric non-viral vector to boost effective tumor immunotherapy. Poly(amidoamine) dendrimers of generation 5 were partially decorate...
Source: Biomacromolecules - March 2, 2022 Category: Biochemistry Authors: Zhijun Ouyang Yue Gao Rui Yang Mingwu Shen Xiangyang Shi Source Type: research