Filtered By:
Source: Nanomedicine : Nanotechnology, Biology, and Medicine

This page shows you your search results in order of relevance. This is page number 2.

Order by Relevance | Date

Total 55 results found since Jan 2013.

Rational modification of oligoarginine for highly efficient siRNA delivery: structure–activity relationship and mechanism of intracellular trafficking of siRNA
Recently, cell-penetrating peptides (CPPs) have received much attention for cellular delivery of therapeutic molecules. However, in the case of CPPs as carriers for siRNA delivery, their utility is often restricted by low cellular uptake and/or entrapment in endosomes. Here, in order to deliver siRNAs with high efficiency, oligoarginine, a prominent member in CPPs, is rationally modified with oligohistidine and stearyl moieties (STR-) by fully taking into account the formation of nanoparticles, uptake and intracellular trafficking.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - September 2, 2014 Category: Nanotechnology Authors: Dafeng Chu, Wen Xu, Ran Pan, Yong Ding, Weiping Sui, P. Chen Source Type: research

Multifunctional Selenium Nanoparticles: Chiral Selectivity of Delivering MDR-siRNA for Reversal of Multidrug Resistance and Real-time Biofluorescence Imaging
In this paper, chiral selenium nanoparticles (L-SeNPs/D-SeNPs) modified with a dinuclear Ruthenium (II) complex were used to effectively deliver siRNA targeting the MDR1 gene. In this co-delivery system, the luminescent dinuclear Ruthenium (II) complex was developed to act as a gene carrier and anti-tumor drug, while offering luminescent imaging to follow the intracellular trafficking. Interestingly, Ru@L-SeNPs exhibited a stronger protein and pDNA affinity then Ru@D-SeNPs, indicating that chirality may have an effect on pDNA/siRNA binding and biocompatibility.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - May 7, 2015 Category: Nanotechnology Authors: Qingchang Chen, Qianqian Yu, Yanan Liu, Dhairya Bhavsar, Licong Yang, Xiaofan Ren, Dongdong Sun, Wenjing Zheng, Jie Liu, Lan-mei Chen Source Type: research

Designing idiosyncratic -siRNA Nanoformulated Capsules for Silencing and Cancer Therapy
In this work, we have designed a siRNA-nanoformulation with mesoporous polycaprolactone (hmPCL) for silencing and cancer therapy. Average hollow core size of hmPCL nanocapsules used for nanoformulation is ~180 nm with shell thickness of 10-20 nm and mesopore size of ~5-10 nm in diameter. Idiosyncratic capsules are biocompatible which has been confirmed with normal lymphocyte, K562 leukaemia cancer cells and on HepG2/EGFP cancer cells. In 1 mg of hmPCL capsules up to 400 ng of siRNA can be loaded.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - November 7, 2015 Category: Nanotechnology Authors: Vijay Bhooshon Kumar, Himadri Medhi, Zhang Yong, Pradip Paik Source Type: research

Robust neuroprotective effects of intranasally delivered iNOS siRNA encapsulated in gelatin nanoparticles in the postischemic brain
The therapeutic efficacy of intranasal iNOS siRNA delivery was investigated in the postischemic rat brain after encapsulating on in gelatin nanoparticles (GNPs; diameter 188.0±60.9 nm) cross-linked with 0.0667% glutaraldehyde (GA). Intranasally delivered GNPs were found in extracellular and intracellular compartments of many brain regions, including the olfactory bulb, cerebral cortex, and striatum at 1 hr after infusion and continued to be detected for days. Infarct volumes were markedly suppressed (maximal reduction to 42.1±2.6%) at 2 days after 60 min of middle cerebral artery occlusion (MCAO) when iNOS siRNA/GNPs wer...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - March 2, 2016 Category: Nanotechnology Authors: Il-Doo Kim, Elizabeth Sawicki, Hye-Kyung Lee, Eun-Hwa Lee, Heon Joo Park, Pyung-Lim Han, Kyekyoon (Kevin) Kim, Hyungsoo Choi, Ja-Kyeong Lee Source Type: research

Inhibition of hepatitis C virus in mouse models by lipidoid nanoparticle-mediated systemic delivery of siRNA against PRK2
Host-targeting antivirals have an advantage over direct-acting antivirals in that they have a high genetic barrier to resistance. Here, we describe in vivo anti-hepatitis C virus (HCV) efficacy of a potent siRNA targeting the protein kinase C-related kinase 2 (PRK2), which phosphorylates HCV NS5B RNA-dependent RNA polymerase and promotes HCV replication. PRK2-silencing reduced the phosphorylated NS5B level and resulted in inhibition of NS5B RdRp activity to decrease HCV genome abundance. Systemic administration of lipidoid nanoparticle-formulated PRK2 siRNA (once every three days for a total of three injections at a dose o...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - March 21, 2016 Category: Nanotechnology Authors: Jae-Su Moon, Seung-Hoon Lee, Song-Hee Han, Eun-Jung Kim, Hee Cho, Wooseong Lee, Mi-Kyung Kim, Tae-Eun Kim, Hyun-Ji Park, Jin-Kyu Rhee, Seong-Jun Kim, Seung-Woo Cho, Seung Hyun Han, Jong-Won Oh Source Type: research

Liposome-polyethylenimine complexes (DPPC-PEI lipopolyplexes) for therapeutic siRNA delivery in vivo
Therapeutic applications of RNA interference (RNAi) require efficient siRNA delivery strategies in vivo. Combining lipid-based carriers with polymeric nanoparticles offers the favourable properties of both systems. This is the first study to explore polyethylenimine-based lipopolyplexes comprising a low-molecular weight PEI and the phospholipid DPPC for therapeutic siRNA use. Lipopolyplex structures are analysed by electron microscopy. Biological efficacies are demonstrated in vitro by cellular uptake, knockdown of the target oncogene survivin and concomitant cell growth inhibition.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - August 20, 2016 Category: Nanotechnology Authors: Alexander Ewe, Omkar Panchal, Shashank Reddy Pinnapireddy, Udo Bakowsky, Susanne Przybylski, Achim Temme, Achim Aigner Source Type: research

Local delivery of siRNA-loaded calcium phosphate nanoparticles abates pulmonary inflammation
The local interference of cytokine signaling mediated by siRNA-loaded nanoparticles might be a promising new therapeutic approach to dampen inflammation during pulmonary diseases. For the local therapeutic treatment of pulmonary inflammation, we produced multi-shell nanoparticles consisting of a calcium phosphate core, coated with siRNAs directed against pro-inflammatory mediators, encapsulated into poly(lactic-co-glycolic acid), and coated with a final outer layer of polyethylenimine. Nasal instillation of nanoparticles loaded with a mixture of siRNAs directed against different cytokines to mice suffering from TH1 cell-me...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - August 8, 2017 Category: Nanotechnology Authors: Annika Frede, Bernhard Neuhaus, Torben Knuschke, Munisch Wadwa, Sebastian Kollenda, Robert Klopfleisch, Wiebke Hansen, Jan Buer, Dunja Bruder, Matthias Epple, Astrid M. Westendorf Source Type: research

Theranostic poly(lactic-co-glycolic acid) nanoparticle for magnetic resonance / infrared fluorescence bimodal imaging and efficient siRNA delivery to macrophages and its evaluation in a kidney injury model
In this work, a theranostic nanoparticle was developed for multimodal imaging and siRNA delivery. The core of the nanoparticles (NP) was formed by encapsulation of superparamagnetic iron oxides and indocyanine green in a PLGA matrix to serve as a multimodal probe for near-infrared (NIFR) and magnetic resonance (MR) imaging. The surface of the particle was coated with polyethylenimine (PEI) for siRNA delivery. Macrophages efficiently took up the nanoparticles and emitted strong NIFR and MR contrast.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - August 22, 2017 Category: Nanotechnology Authors: Chuanxu Yang, Hieu Vu-Quang, Dina Michelle Unnerup Husum, Stine Julie Tingskov, Mads Sloth Vinding, Thomas Nielsen, Ping Song, Niels Chr. Nielsen, Rikke N ørregaard, Jørgen Kjems Source Type: research

Theranostic poly(lactic-co-glycolic acid) nanoparticle for magnetic resonance/infrared fluorescence bimodal imaging and efficient siRNA delivery to macrophages and its evaluation in a kidney injury model
In this work, a theranostic nanoparticle was developed for multimodal imaging and siRNA delivery. The core of the nanoparticles (NP) was formed by encapsulation of superparamagnetic iron oxides and indocyanine green in a PLGA matrix to serve as a multimodal probe for near-infrared (NIFR) and magnetic resonance (MR) imaging. The surface of the particle was coated with polyethylenimine (PEI) for siRNA delivery. Macrophages efficiently took up the nanoparticles and emitted strong NIFR and MR contrast.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - August 22, 2017 Category: Nanotechnology Authors: Chuanxu Yang, Hieu Vu-Quang, Dina Michelle Unnerup Husum, Stine Julie Tingskov, Mads Sloth Vinding, Thomas Nielsen, Ping Song, Niels Chr. Nielsen, Rikke N ørregaard, Jørgen Kjems Source Type: research

Development of a Peptide-Modified siRNA Nanocomplex for Hepatic Stellate Cells
Insulin-like growth factor 2 receptor (IGF2R) is overexpressed in activated Hepatic stellate cells (HSCs) and therefore can be utilized for HSC-specific drug delivery. We recently discovered an IGF2R-specific peptide using a novel biopanning. Here, we adopted biotin-conjugated IGF2R-specific peptide, cholesterol, and vitamin A as the targeting ligands for the neutravidin-based siRNA nanocomplex to deliver PCBP2 siRNA, a potentially antifibrotic agent, to HSCs. Compared to Vitamin A and cholesterol, the IGF2R-specific peptide exhibited the highest targeting effect to human LX-2 HSC, rat HSC-T6 cell line, and activated primary rat HSCs.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - September 7, 2017 Category: Nanotechnology Authors: Zhen Zhao, Yuanke Li, Akshay Jain, Zhijin Chen, Hao Liu, Wei Jin, Kun Cheng Source Type: research

Intraperitoneal Nanotherapy for Metastatic Ovarian Cancer Based on siRNA-Mediated Suppression of DJ-1 Protein Combined with a Low Dose of Cisplatin
Herein, we report an efficient combinatorial therapy for metastatic ovarian cancer based on siRNA-mediated suppression of DJ-1 protein combined with a low dose of cisplatin. DJ-1 protein modulates, either directly or indirectly, different oncogenic pathways that support and promote survival, growth, and invasion of ovarian cancer cells. To evaluate the potential of this novel therapy, we have engineered a cancer-targeted nanoplatform and validated that DJ-1 siRNA delivered by this nanoplatform after intraperitoneal injection efficiently downregulates the DJ-1 protein in metastatic ovarian cancer tumors and ascites.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - April 7, 2018 Category: Nanotechnology Authors: Canan Schumann, Stephanie Chan, Jess A. Millar, Yuliya Bortnyak, Katherine Carey, Alex Fedchyk, Leon Wong, Tetiana Korzun, Abraham S. Moses, Anna Lorenz, Delany Shea, Olena Taratula, Oleh Khalimonchuk, Oleh Taratula Source Type: research

Enhancing the therapeutic effect via elimination of hepatocellular carcinoma stem cells using Bmi1 siRNA delivered by cationic cisplatin Nanocapsules
Resistance of hepatocellular carcinoma (HCC) to systemic chemotherapy is partially due to presence of drug-resistant cancer stem cells. Bmi1 protein is essential for survival and proliferation of HCC cancer stem cells (CSCs). Here, we report that Bmi1 siRNA (Bmi1siR) loaded in cationic nanocapsules of cisplatin (NPC) eliminated stem cells in situ HCC in mice. NPC/Bmi1siR was fabricated via electrostatic complexation of Bmi1 siRNA to NPCs, which had cores composed of cisplatin and were coated with cationic lipids.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - May 26, 2018 Category: Nanotechnology Authors: Tan Yang, Yuyuan Chen, Pengxuan Zhao, Huiying Xue, Jia You, Bin Li, Yong Liu, Chuanchuan He, Xiaojuan Zhang, Lingling Fan, Robert J. Lee, Lei Li, Xiang Ma, Chuanrui Xu, Guangya Xiang Source Type: research

Cationic lipid nanoparticles for therapeutic delivery of siRNA and miRNA to murine liver tumor
In this study, LNP-DP1 –a cationic lipid nanoparticle formulation –is reported as a vehicle to restore deregulated gene expression in hepatic carcinoma cells by siRNA and miRNA delivery using a mouse model. Further expansions to this study may enable transition to clinical trials of this system.Graphical Abstract: The schematic representation of mi-/si-RNA encapsulated by PEG modified LNP-DP1 which is mainly composed of EggPC, cholesterol and cationic lipid DODMA. The LNP-DP1 (red particles) can be specifically and efficiently taken up by hepatocytes and tumor cells (blue nuclei and green cell outline) after delivery t...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - June 3, 2013 Category: Nanotechnology Authors: Shu-hao Hsu, Bo Yu, Xinmei Wang, Yuanzhi Lu, Carl R. Schmidt, Robert J. Lee, L. James Lee, Samson T. Jacob, Kalpana Ghoshal Tags: Genetics, Gene Delivery, HEP-CC, Micro-RNA Delivery, Cationic Lipid NPs Source Type: research

Mesoporous silica nanoparticle delivery of chemically modified siRNA against TWIST1 leads to reduced tumor burden
Growth and progression of solid tumors depends on the integration of multiple pro-growth and survival signals, including the induction of angiogenesis. TWIST1 is a transcription factor whose reactivation in tumors leads to epithelial to mesenchymal transition (EMT), including increased cancer cell stemness, survival, and invasiveness. Additionally, TWIST1 drives angiogenesis via activation of IL-8 and CCL2, independent of VEGF signaling. In this work, results suggest that chemically modified siRNA against TWIST1 reverses EMT both in vitro and in vivo.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - June 23, 2015 Category: Nanotechnology Authors: James Finlay, Cai M. Roberts, Juyao Dong, Jeffrey I. Zink, Fuyuhiko Tamanoi, Carlotta A. Glackin Source Type: research