• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Inhibition of pre-B cell colony-enhancing factor attenuates inflammation induced by hyperoxia in EA.hy926 cells.
Abstract The aim of this study was to investigate the role of pre-B cell colony-enhancing factor (PBEF) in the pathogenesis of bronchopulmonary dysplasia (BPD) using an established cell model of BPD. For this purpose, EA.hy926 cell cultures were divided into 4 groups as follows: the air group as the blank control, the hyperoxia group, the hyperoxia plus PBEF siRNA group and the hyperoxia plus scramble siRNA group. Cell viability and the generation of reactive oxygen species (ROS) were determined using respective kits. Moreover, the protein and mRNA expression levels of PBEF, interleukin-8 (IL-8) and tumor nec...
Source: International Journal of Molecular Medicine - June 29, 2017 Category: Molecular Biology Authors: He JY, Cui HJ, Tang LJ, Chen J, Huang WM Tags: Int J Mol Med Source Type: research

miR ‑142‑3p targets AC9 to regulate sciatic nerve injury‑induced neuropathic pain by regulating the cAMP/AMPK signalling pathway.
miR‑142‑3p targets AC9 to regulate sciatic nerve injury‑induced neuropathic pain by regulating the cAMP/AMPK signalling pathway. Int J Mol Med. 2020 Dec 16;: Authors: Li X, Wang S, Yang X, Chu H Abstract The aim of the present study was to investigate the effects of microRNA (miR)‑142‑3p on neuropathic pain caused by sciatic nerve injury in chronic compression injury (CCI) rats, and further investigate its mechanism. Rat experiments were divided into four parts in the study. In the first part, the rats were divided into the Sham and CCI groups. The expression of miR‑142‑3p, AC9 and cAMP...
Source: International Journal of Molecular Medicine - December 16, 2020 Category: Molecular Biology Authors: Li X, Wang S, Yang X, Chu H Tags: Int J Mol Med Source Type: research

Oxymatrine attenuates oxidized low ‑density lipoprotein‑induced HUVEC injury by inhibiting NLRP3 inflammasome‑mediated pyroptosis via the activation of the SIRT1/Nrf2 signaling pathway
Int J Mol Med. 2021 Oct;48(4):187. doi: 10.3892/ijmm.2021.5020. Epub 2021 Aug 9.ABSTRACTOxymatrine, a quinolizidine alkaloid isolated from the traditional Chinese herb Sophora flavescens Aiton, has been demonstrated to exert anti‑inflammatory and atherosclerotic effects, but the molecular mechanism has yet to be elucidated. Accumulating evidence indicates an important role of NLR family pyrin domain containing 3 (NLRP3) inflammasome‑mediated pyroptosis in the pathogenesis of atherosclerosis. The present study was undertaken to investigate whether oxymatrine attenuates oxidized low‑density lipoprotein (ox‑LDL)‑ind...
Source: International Journal of Molecular Medicine - August 9, 2021 Category: Molecular Biology Authors: Xin Jin Wan Fu Jiaxiu Zhou Niannian Shuai Yan Yang Bo Wang Source Type: research

Downregulation of Notch1 inhibits the invasion of human hepatocellular carcinoma HepG2 and MHCC97H cells through the regulation of PTEN and FAK.
Abstract Tumor invasion and metastasis are the main causes of mortality in patients with hepatocellular carcinoma (HCC). Thus, the effective inhibition of these tumorigenic processes is critical in order for HCC therapy to be effective. Previous studies have demonstrated that Notch1 is associated with metastasis in several human malignancies. However, the exact molecular mechanisms underlying the Notch1-mediated induction of the invasion of HCC cells remain poorly understood. In the present study, we demonstrate that, compared to the normal liver cell line, L02, Notch1 is highly expressed in the human HCC cell lin...
Source: International Journal of Molecular Medicine - August 8, 2014 Category: Molecular Biology Authors: Hu YJ, Li HY, Qiu KJ, Li DC, Zhou JH, Hu YH, Zhang FM Tags: Int J Mol Med Source Type: research

P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation.
In conclusion, our results demonstrate that P2X7R plays a significant role in the development of atherosclerosis and regulates NLRP3 inflammasome activation by promoting PKR phosphorylation. PMID: 25761252 [PubMed - as supplied by publisher]
Source: International Journal of Molecular Medicine - March 9, 2015 Category: Molecular Biology Authors: Peng K, Liu L, Wei D, Lv Y, Wang G, Xiong W, Wang X, Altaf A, Wang L, He D, Wang H, Qu P Tags: Int J Mol Med Source Type: research

The augmentation of O-GlcNAcylation reduces glyoxal-induced cell injury by attenuating oxidative stress in human retinal microvascular endothelial cells.
Abstract It has recently been reported that O-linked β-N-acetyl glucosamine (O-GlcNAc) modification (a simple intracellular serine (Ser)/threonine (Thr)-linked monosaccharide) in human retinal microvascular endothelial cells (HRECs) is related to diabetic retinopathy (DR). During O-GlcNAcylation, O-GlcNAc is added to Ser and Thr residues. As the generation of reactive oxygen species (ROS) is one of the characteristics of advanced glycation end product (AGE) injury, and the most important key pathogenic factor of DR, in the present study, we aimed to investigate the association between O-GlcNAcylation and RO...
Source: International Journal of Molecular Medicine - August 20, 2015 Category: Molecular Biology Authors: Liu GD, Xu C, Feng L, Wang F Tags: Int J Mol Med Source Type: research

CNTF protects neurons from hypoxic injury through the activation of STAT3pTyr705.
Abstract The aim of the present study was to investigate whether ciliary neurotrophic factor (CNTF) plays its neuroprotective role following hypoxic injury through the activation of signal transducer and activator of transcription 3 (STAT3) signaling. Firstly, to determine whether CNTF exerts its effects via STAT3 following hypoxic injury, cultured neurons from the cerebral cortex of mice were prepared and a neuronal model of hypoxia was then established. The neurons exposed to hypoxia were then pre-treated with CNTF and transfected with small interference RNA (siRNA) targeting STAT3 (STAT3 siRNA) using poly...
Source: International Journal of Molecular Medicine - October 12, 2016 Category: Molecular Biology Authors: Gu YL, Gao GQ, Ma N, Ye LL, Zhang LW, Gao X, Zhang ZB Tags: Int J Mol Med Source Type: research

Kif4 regulates the expression of VEGFR1 through the PI3K/Akt signaling pathway in RAW264.7 monocytes/macrophages.
Abstract Kinesin superfamily protein 4 (Kif4), a microtubule-based motor protein, has been shown to participate in a number of critical cellular processes, such as cell division, the intracellular transport of membranous vesicles and signal transduction. However, whether KIF4 regulates vascular endothelial growth factor (VEGF) receptor 1 (VEGFR1) expression remains unknown. Thus, in this study, in order to examine the effects of Kif4 on the expression of VEGFR1 in RAW264.7 monocytes/macrophages, Kif4 was silenced using siRNA. RT-qPCR, western blot analysis and ELISA were used to assess the expression of Kif4 ...
Source: International Journal of Molecular Medicine - March 27, 2017 Category: Molecular Biology Authors: Xu Y, Luan Y, Liu S, Sun J, Wang K, Cai J, Jiang W, Yang P, Wei F, Qu X Tags: Int J Mol Med Source Type: research

Suppression of CIP4/Par6 attenuates TGF- β1-induced epithelial-mesenchymal transition in NRK-52E cells.
In this study, the Cdc42-interacting protein-4 (CIP4)/partitioning-defective protein 6 (Par6) pathway was investigated in TGF-β1‑stimulated NRK-52E cells. Rat NRK-52E cells were obtained and stimulated with TGF-β1. The expression levels of E-cadherin, α-smooth muscle actin (α-SMA) and CIP4 were then examined by western blot analyses. Rat NRK-52E cells were transfected with Par6 or CIP4 small interfering RNA (siRNA), and scrambled siRNA as controls. The cells were incubated with 20 ng/ml of TGF-β1 for 72 h in order to observe the effects of Par6 and CIP4 silencing. Confocal fluorescence microscopy was also ...
Source: International Journal of Molecular Medicine - August 16, 2017 Category: Molecular Biology Authors: Zhu YC, Wang YK, Bai SJ, Zha FF, Feng G, Gao CP, Liu J Tags: Int J Mol Med Source Type: research

Pages: 1  2  3  4  5  6  7  8  9  10  11  12  13  14