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Source: The International Journal of Biochemistry and Cell Biology

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Total 120 results found since Jan 2013.

YAP1 negatively regulates chondrocyte differentiation partly by activating the β-catenin signaling pathway.
Abstract YAP1 (Yes-associated protein 1) transcriptional coactivator is a downstream gene of the Hippo signaling pathway, which controls cell proliferation and differentiation. YAP1 plays a significant role in the regulation of cartilage and bone development. However, the molecular mechanism by which YAP1 regulates chondrocyte differentiation remains to be elucidated. Immunofluorescent staining was used to visualize the localization of YAP1 expression in the mouse chondroprogenitor ATDC5 cell line. ATDC5 cells with lentivirus-vector-mediated YAP1 overexpression and knockdown were established. The differentiation a...
Source: The International Journal of Biochemistry and Cell Biology - April 21, 2017 Category: Biochemistry Authors: Yang B, Sun H, Song F, Yu M, Wu Y, Wang J Tags: Int J Biochem Cell Biol Source Type: research

mROS-TXNIP axis activates NLRP3 inflammasome to mediate renal injury during ischemic AKI.
Abstract Ischemia/reperfusion (I/R) is a critical risk factor for acute kidney injury (AKI). Recent studies provided evidence that tubular epithelial cells (TEC)-associated inflammation aggravates kidney injury and impairs tissue repair after I/R injury. Here we demonstrated that the Nod-like receptor protein 3 (NLRP3) inflammasome is activated by mitochondrial reactive oxygen species (mROS) during I/R injury via direct interactions between the inflammasome and thioredoxin-interacting protein (TXNIP). Firstly, we found that NLRP3 inflammasome activation was induced by I/R injury, peaking at day 3 after reperfusion...
Source: The International Journal of Biochemistry and Cell Biology - February 22, 2018 Category: Biochemistry Authors: Wen Y, Liu YR, Tang TT, Pan MM, Xu SC, Ma KL, Lv LL, Liu H, Liu BC Tags: Int J Biochem Cell Biol Source Type: research

Cyclic Biaxial Tensile Strain Promotes Bone Marrow-Derived Mesenchymal Stem Cells to Differentiate into Cardiomyocyte-like Cells by miRNA-27a.
Abstract A physical stimuli, it has been reported that cyclic tensile strain can promote bone marrow-derived mesenchymal stem cells (BMSCs) to differentiate into cardiomyocytes, but the underlying mechanisms have been poorly elucidated so far. Here, we used a mimicking loading strain, cyclic biaxial tensile strain (CBTS), and found it can promote BMSCs to differentiate into cardiomyocytes. When the CBTS were loaded, the cells expressed cardiac-specific markers GATA4, TNNT2, MEF-2c, and Cx43, meanwhile we found miR-27a decreased and stem cell factor (SCF) increased. When we overexpressed miR-27a, the cardiac-specif...
Source: The International Journal of Biochemistry and Cell Biology - April 5, 2018 Category: Biochemistry Authors: Cao C, Li L, Li H, He X, Geng W, Xiaoqin Y Tags: Int J Biochem Cell Biol Source Type: research

Inhibitory effect of microRNA-154 targeting WHSC1 on cell proliferation of human skin squamous cell carcinoma through mediating the P53 signaling pathway.
CONCLUSION: These results demonstrate that up-regulation of miR-154 inhibits proliferation and induces apoptosis of human skin SCC cells by down-regulating WHSC1 and blocking the P53 signaling pathway. PMID: 29727714 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - May 1, 2018 Category: Biochemistry Authors: Chen HQ, Gao D Tags: Int J Biochem Cell Biol Source Type: research

Long non-coding RNA CCAT1 is overexpressed in endometrial cancer and regulates growth and transcriptome of endometrial adenocarcinoma cells.
CONCLUSIONS: Given that the lncRNA CCAT1 was found to be overexpressed in endometrial cancer, affected the growth of HEC-1B cells and the expression of growth regulatory genes, our data suggest CCAT1 to exert oncogenic functions in endometrial cancer and encourage further studies to examine to what extent this lncRNA might be a potential therapy target in this cancer entity. PMID: 32173521 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - March 11, 2020 Category: Biochemistry Authors: Treeck O, Skrzypczak M, Schüler-Toprak S, Weber F, Ortmann O Tags: Int J Biochem Cell Biol Source Type: research

Prostaglandin E2 induces stromal cell-derived factor-1 expression in prostate stromal cells by activating protein kinase A and transcription factor Sp1.
In conclusion, we provide evidence for a paracrine prostate stromal-epithelial interaction induced by upregulation of expression of SDF-1 by PGE2. Our research provides new insights into the mechanism promoting metastasis of prostate carcinoma via stromal-epithelial interaction. PMID: 23246486 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - December 11, 2012 Category: Biochemistry Authors: Peng Y, Shi J, Du X, Wang L, Klocker H, Mo L, Mo Z, Zhang J Tags: Int J Biochem Cell Biol Source Type: research

Overexpression of human selenoprotein H in neuronal cells enhances mitochondrial biogenesis and function through activation of protein kinase A, protein kinase B, and cyclic adenosine monophosphate response element-binding protein pathway.
The objective of this study is to identify the cell signaling pathways by which selenoprotein H initiates mitochondrial biogenesis. We first confirmed our previous observation that selenoprotein H transfected HT22 cells increased the protein levels of nuclear-encoded mitochondrial biogenesis factors, peroxisome proliferator-activated receptor γ coactivator-1α, nuclear respiratory factor 1 and mitochondrial transcription factor A. We then observed that total and phosphorylation of protein kinase A, Akt/protein kinase B and cyclic adenosine monophosphate response element-binding protein (CREB) were significantly increased ...
Source: The International Journal of Biochemistry and Cell Biology - December 7, 2012 Category: Biochemistry Authors: Mehta SL, Mendelev N, Kumari S, Andy Li P Tags: Int J Biochem Cell Biol Source Type: research

NF-κB p65 and c-Rel subunits promote phagocytosis and cytokine secretion by splenic macrophages in cirrhotic patients with hypersplenism.
In this study, we explored the role of NF-κB in the dysfunction of splenic macrophages in hypersplenism due to liver cirrhosis. By using confocal microscopic analysis, Western Blot, TransAM NF-κB ELISA, and chromatin immunoprecipitation (ChIP), we observed that NF-κB p65, p52, and c-Rel were activated in macrophages in patients with hypersplenism (hypersplenic macrophages). Transfection of hypersplenic macrophages with a κB/luciferase reporter plasmid showed that NF-κB complexes were functional. Using co-immunoprecipitation studies, we demonstrated that p65/c-Rel dimers were activated in hypersplenic macrophages. NF-Î...
Source: The International Journal of Biochemistry and Cell Biology - November 26, 2012 Category: Biochemistry Authors: Ren S, Zhang S, Li M, Huang C, Liang R, Jiang A, Guo Y, Pu Y, Huang N, Yang J, Li Z Tags: Int J Biochem Cell Biol Source Type: research

Retinoic acid receptor beta mediates all-trans retinoic acid facilitation of mesenchymal stem cells neuronal differentiation.
Abstract All-trans retinoic acid plays an important role in nervous system development. However, the effects of all-trans retinoic acid on the neuronal differentiation of mesenchymal stem cells and the mechanisms through which this differentiation takes place are still poorly understood. Here, we investigated the biological effects of all-trans retinoic acid on the neuronal differentiation of mesenchymal stem cells and the signaling pathways that mediated these effects. We found that the neuronal differentiation efficiency of mesenchymal stem cells following all-trans retinoic acid pre-induction was greater and th...
Source: The International Journal of Biochemistry and Cell Biology - January 11, 2013 Category: Biochemistry Authors: Gong M, Bi Y, Jiang W, Zhang Y, Chen L, Hou N, Chen J, Li T Tags: Int J Biochem Cell Biol Source Type: research

Transcription factor AP-2α regulates acute myeloid leukemia cell proliferation by influencing Hoxa gene expression.
Abstract Transcription factor AP-2α mediates transcription of a number of genes implicated in mammalian development, cell proliferation and carcinogenesis. In the current study, we identified Hoxa7, Hoxa9 and Hox cofactor Meis1 as AP-2α target genes, which are involved in myeloid leukemogenesis. Luciferase reporter assays revealed that overexpression of AP-2α activated transcription activities of Hoxa7, Hoxa9 and Meis1, whereas siRNA of AP-2α inhibited their transcription activities. We found that AP-2 binding sites in regulatory regions of three genes activated their transcription by mutant analysis and AP-2Î...
Source: The International Journal of Biochemistry and Cell Biology - May 6, 2013 Category: Biochemistry Authors: Ding X, Yang Z, Zhou F, Wang F, Li X, Chen C, Li X, Hu X, Xiang S, Zhang J Tags: Int J Biochem Cell Biol Source Type: research

Two p53-related metabolic regulators, TIGAR and SCO2, contribute to oroxylin A-mediated glucose metabolism in human hepatoma HepG2 cells.
This study revealed a new aspect of glucose metabolism regulation of oroxylin A, which may contribute to its new anticancer mechanism. PMID: 23612020 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - April 21, 2013 Category: Biochemistry Authors: Dai Q, Yin Y, Liu W, Wei L, Zhou Y, Li Z, You Q, Lu N, Guo Q Tags: Int J Biochem Cell Biol Source Type: research

Variants of the electrogenic sodium bicarbonate cotransporter 1 (NBCe1) in mouse hippocampal neurons are regulated by extracellular pH changes: Evidence for a Rab8a-dependent mechanism.
Abstract Changes in extracellular pH are common events in both pathological conditions and during normal brain function. In organs other than the brain, cells may respond to pH changes by trafficking of acid-base transporters. However, regulation of neuronal acid-base transporters during pH shifts is not understood. The aim of this study was to investigate regulatory mechanisms of the variants of the electrogenic sodium/bicarbonate cotransporter 1, NBCe1-A and NBCe1-B/C, in neurons following changes of extracellular pH. Therefore, primary mouse hippocampal neurons were exposed to extracellular acidosis or alkalosi...
Source: The International Journal of Biochemistry and Cell Biology - April 11, 2013 Category: Biochemistry Authors: Oehlke O, Speer JM, Roussa E Tags: Int J Biochem Cell Biol Source Type: research

15-Lipoxygenase-1/15-hydroxyeicosatetraenoic acid promotes hepatocellular cancer cells growth through protein kinase B and heat shock protein 90 complex activation.
Abstract Hepatocellular carcinoma is a typical hypervascular tumor resulted from excessive growth of tumor cells. Previous studies have demonstrated that the lipoxygenase is considered as a potential therapeutic target and have important influence on human cancers. However, whether the 15-lipoxygenase-1 (15-LO-1)/15-hydroxyeicosatetraenoic acid (15-HETE) pathway participates in the development and progression of hepatocellular carcinoma has not been reported until now. To test the hypothesis that the 15-LO-1/15-HETE signaling regulates hepatocellular carcinoma cells growth and metastasis via the phosphoinositide-3...
Source: The International Journal of Biochemistry and Cell Biology - May 23, 2013 Category: Biochemistry Authors: Ma J, Zhang L, Zhang J, Liu M, Wei L, Shen T, Ma C, Wang Y, Chen Y, Zhu D Tags: Int J Biochem Cell Biol Source Type: research

The self-renewal of mouse embryonic stem cells is regulated by cell-substratum adhesion and cell spreading.
Abstract Mouse embryonic stem cells (mESCs) undergo self-renewal in the presence of the cytokine, leukaemia inhibitory factor (LIF). Following LIF withdrawal, mESCs differentiate, and this is accompanied by an increase in cell-substratum adhesion and cell spreading. The purpose of this study was to investigate the relationship between cell spreading and mESC differentiation. Using E14 and R1 mESC lines, we have restricted cell spreading in the absence of LIF by either culturing mESCs on chemically defined, weakly adhesive biomaterial substrates, or by manipulating the cytoskeleton. We demonstrate that by restricti...
Source: The International Journal of Biochemistry and Cell Biology - July 17, 2013 Category: Biochemistry Authors: Murray P, Prewitz M, Hopp I, Wells N, Zhang H, Cooper A, Parry KL, Short R, Antoine DJ, Edgar D Tags: Int J Biochem Cell Biol Source Type: research

NBPF is a potential DNA-binding transcription factor that is directly regulated by NF-κB.
In this study, the genes of NBPF family were found to be densely covered by many high-confidence ChIP-Seq peaks of NF-κB. The expressions of NBPF genes were thus deduced to be regulated by this transcription factor. The activities of NF-κB in HeLa, HepG2 and ECa109 cells were then manipulated with NF-κB activator (TNFα) and inhibitors (BAY11-7082 or p65 siRNA), and the expressions of NBPF genes in these cells were checked. As result, it was found that the expressions of NBPF genes were regulated by NF-κB in HeLa and HepG2 cells. Therefore, the genes of NBPF family were identified as new bona fide direct target genes o...
Source: The International Journal of Biochemistry and Cell Biology - August 9, 2013 Category: Biochemistry Authors: Zhou F, Xing Y, Xu X, Yang Y, Zhang J, Ma Z, Wang J Tags: Int J Biochem Cell Biol Source Type: research