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MKP-1 is a target of miR-210 and mediate the negative regulation of miR-210 inhibitor on hypoxic hPASMC proliferation.
Abstract Chonic hypoxia, smooth muscle cell (SMC) proliferation and vascular remodeling are hallmark features of pathogenic pulmonary artery hypertension. MicroRNAs (miRNAs), endogenously expressed small noncoding RNAs, regulate gene expression at the post-transcriptional level. MiR-210 is considered a "master miRNA" in the control of diverse functions in hypoxic cells and tissues and has a cytoprotective function in pulmonary artery SMCs during hypoxic stress. MiR-210 is also upregulated in lung tissue of chonically hypoxic mice suffering from pulmonary hypertension. Yi et al. (XXXX) showed that mice deficient in...
Source: Cell Biology International - July 15, 2014 Category: Cytology Authors: Jin Y, Pang T, Nelin LD, Wang W, Wang Y, Yan J, Zhao C Tags: Cell Biol Int Source Type: research

Knockdown of tumor protein D52-like 2 induces cell growth inhibition and apoptosis in oral squamous cell carcinoma.
Abstract Tumor protein D52-like 2 (TPD52L2) and its family members form homo- and heteromeric complexes essential for cell proliferation in multiple human cancers. TPD52L2 is involved in cell migration and attachment in oral squamous cell carcinoma (OSCC). To confirm the role of TPD52L2 in OSCC, we employed the lentivirusdelivered small interfering RNA (siRNA) technique to knock down TPD52L2 expression in 2 OSCC cell lines, CAL27 and KB. Knockdown of TPD52L2 by RNA interference markedly suppressed cell proliferation and colony formation. Cell cycle analysis showed that depletion of TPD52L2 led to CAL27 cells arres...
Source: Cell Biology International - September 27, 2014 Category: Cytology Authors: He Y, Chen F, Cai Y, Chen S Tags: Cell Biol Int Source Type: research

Gab1 amplifies signalling in response to low-intensity stimulation by HGF.
Abstract The receptor tyrosine kinases EGFR and Met induce phosphorylation of the docking protein Gab1, and there is evidence that Gab1 may have a role in the signalling from these receptors. Studying hepatocytes, we previously found that although Gab1 mechanistically interacted in different ways with EGFR and Met, it was involved in mitogenic signalling induced by both EGF and HGF. It has been reported that in EGFR signalling Gab1 is required particularly at a low dose of EGF. Whether this also applies to HGF/Met signalling has not been investigated. We have studied the role of Gab1 in activation of the Akt and E...
Source: Cell Biology International - July 4, 2015 Category: Cytology Authors: Aasrum M, Ødegård J, Thoresen GH, Brusevold IJ, Sandnes DL, Christoffersen T Tags: Cell Biol Int Source Type: research

IRE1α and TRB3 do not contribute to the disruption of proximal insulin signaling caused by palmitate in C2C12 myotubes.
Abstract Endoplasmic reticulum (ER) stress is a central actor in the physiopathology of insulin resistance (IR) in various tissues. The subsequent unfolded protein response (UPR) interacts with insulin signaling through inositol-requiring 1α (IRE1α) activation and tribbles homolog 3 (TRB3) expressions. IRE1α impairs insulin actions through the activation of c-Jun N-terminal kinase (JNK) and TRB3 is a pseudokinase inhibiting Akt. In muscle cells, the link between ER stress and IR has only been demonstrated by using chemical ER stress inducers or overexpression techniques. However, the involvement of ER stress in...
Source: Cell Biology International - September 4, 2015 Category: Cytology Authors: Pierre N, Fernández-Verdejo R, Regnier P, Vanmechelen S, Demeulder B, Francaux M Tags: Cell Biol Int Source Type: research

Wnt5A exerts immunomodulatory activity in the human ovarian cancer cell line SKOV-3.
This study revealed a previously unrecognized immunomodulatory role of endogenous Wnt5A in ovarian cancer cells, which could further influence chemotactic cell migration. PMID: 26462870 [PubMed - as supplied by publisher]
Source: Cell Biology International - October 13, 2015 Category: Cytology Authors: Arabzadeh S, Hossein G, Zarnani AH Tags: Cell Biol Int Source Type: research

Cilostazol inhibits interleukin-1-induced ADAM17 expression through cAMP independent signaling in vascular smooth muscle cells.
Abstract Increased a disintegrin and metalloprotease 17 (ADAM17) expression in vascular smooth muscle cells (VSMC) is implicated in the development of cardiovascular diseases including atherosclerosis and hypertension. Although cilostazol, type phosphodiesterase (PDE?) inhibitor, has recently been found to inhibit VSMC proliferation, the mechanisms remain largely unclear. Here we hypothesized that cilostazol regulates the ADAM17 expression in VSMC. In cultured VSMC, interleukin (IL)-1α and IL-1β significantly increased ADAM17 expression. MEK inhibitor U0126, NF-κB inhibitor BAY-11-7085 and siRNA targeting p65/R...
Source: Cell Biology International - October 30, 2015 Category: Cytology Authors: Takaguri A, Morimoto M, Imai SI, Satoh K Tags: Cell Biol Int Source Type: research

Costunolide induces lung adenocarcinoma cell line A549 cells apoptosis through ROS (reactive oxygen species)-mediated endoplasmic reticulum stress.
Abstract Costunolide is an active sesquiterpene lactone derived from many herbal medicines. It has a broad spectrum of bioactivities, including anti-inflammatory and potential anti-tumor effects. The aims of the present study were to evaluate the inhibitory effects of costunolide on A549 cell growth and to explore the underlying molecular mechanisms. Annexin V-FITC/PI flow cytometry analysis revealed that costunolide induced apoptosis. To study the mechanism, we found that costunolide exposure activated the unfolded protein response (UPR) signaling pathways, as shown by the up-regulation of GRP78 and IRE1α and th...
Source: Cell Biology International - November 26, 2015 Category: Cytology Authors: Wang Z, Zhao X, Gong X Tags: Cell Biol Int Source Type: research

Oleanolic acid induces autophagic death in human gastric cancer cells in vitro and in vivo.
In this study, we found that OA induced autophagic cell death in multiple human gastric cancer cell lines. Moreover, OA-induced autophagy was shown for the first time in human gastric cancer cells, evidenced by the formation of GFP-RFP-LC3 puncta and autophagosomes. OA suppressed phospho-mTOR through inhibition of the PI3K/AKT and ERK/p38 MAPK signalling pathways and through activation of the AMPK signalling pathway. Furthermore, we found that OA-induced cytotoxicity and autophagy could be blocked by the autophagy inhibitor 3-methyladenine or via siRNA targeting Beclin-1. Our in vivo research showed that OA delayed the for...
Source: Cell Biology International - April 13, 2016 Category: Cytology Authors: Nie H, Wang Y, Qin Y, Gong XG Tags: Cell Biol Int Source Type: research

Identification of a distinct sub population of fibroblasts from murine dermis: CD73(-) CD105(+) as potential marker of dermal fibroblasts subset with multipotency.
In this study, we isolated dermal cells from the skin of newborn C57/B6 mice and investigated their characteristics. Isolated murine dermal cells exhibited a fibroblast phenotype as judged by accepted criteria including a lack of MSC-related antigens and the differentiation potential of MSCs, and the positive expression of fibroblast markers. A comparative analysis demonstrated that CD73(-) CD105(+) but not CD73(-) CD105(-) dermal fibroblasts exhibited some of the functional properties of MSCs. Furthermore, the multipotent phenotype of CD73(-) CD105(+) cells was diminished by treatment of CD105 siRNA and shRNA, indicating ...
Source: Cell Biology International - May 11, 2016 Category: Cytology Authors: Lee SB, Shim S, Kim MJ, Shin HY, Jang WS, Lee SJ, Jin YW, Lee SS, Park S Tags: Cell Biol Int Source Type: research

H2 S inhibits apo(a) expression and secretion through PKCα/FXR and Akt/HNF4α pathways in HepG2 cells.
This study reveals that H2 S suppressed apo(a) expression and secretion via the PKCα-FXR and PI3K/Akt-HNF4α pathways. PMID: 27298021 [PubMed - as supplied by publisher]
Source: Cell Biology International - June 13, 2016 Category: Cytology Authors: Qu K, Liu YM, He XL, Zhang H, Zhang K, Peng J, Tang YL, Yu XH, Zeng JF, Lei JJ, Wei DH, Wang Z Tags: Cell Biol Int Source Type: research

MiR-154 directly Suppresses DKK2 to Activate Wnt Signaling Pathway and Enhance Activation of Cardiac Fibroblasts.
Abstract Excessive proliferation of cardiac fibroblasts (CFs) and their transdifferentiation into myofibroblasts leads to expression of α-smooth muscle actin (α-SMA), as well as excessive synthesis and secretion of collagens. This process represents an important pathological basis for myocardial fibrosis (MF). MicroRNA (miR)-154 and the Wnt signaling pathway play key roles in the above process, although their specific interactions are poorly understood. After transfecting CFs with miR-154 mimics or inhibitors, miR-154 was found to inhibit the expression of Dickkopf-related protein 2 (DKK2), while miR-154 inhibit...
Source: Cell Biology International - August 19, 2016 Category: Cytology Authors: Sun LY, Bie ZD, Zhang CH, Li H, Li LD, Yang J Tags: Cell Biol Int Source Type: research

AMPK activation by prolonged stimulation with interleukin-1 β contributes to the promotion of GLUT4 translocation in skeletal muscle cells.
AMPK activation by prolonged stimulation with interleukin-1β contributes to the promotion of GLUT4 translocation in skeletal muscle cells. Cell Biol Int. 2016 Aug 29; Authors: Takaguri A, Inoue S, Kubo T, Satoh K Abstract Impaired insulin signaling in skeletal muscle cells causes insulin resistance associated with the onset of type 2 diabetes. Although interleukin (IL)-1β has been considered to be implicated in the pathogenesis of type 2 diabetes, the action of prolonged stimulation with IL-1β on the insulin signaling pathway in skeletal muscle cells remains poorly understood. In the current study,...
Source: Cell Biology International - August 28, 2016 Category: Cytology Authors: Takaguri A, Inoue S, Kubo T, Satoh K Tags: Cell Biol Int Source Type: research

Gene expression profile analysis of dbpA knockdown in colorectal cancer cells.
CONCLUSION: dbpA knockdown in SW620 cells altered the expression of carcinogenesis-associated genes in CRC and the involvement of dbpA on CRC might through MAPK signaling pathway, which might provide valuable evidence to further investigate the correlation between dbpA expression and CRC development. PMID: 27569444 [PubMed - as supplied by publisher]
Source: Cell Biology International - August 28, 2016 Category: Cytology Authors: Liu R, Wang G, Liu C, Qiu J, Yan L, Li X, Wang X Tags: Cell Biol Int Source Type: research

Lipopolysaccharide induces TREM-1-dependent HIF-1 α expression in human keratinocyte cell line.
Lipopolysaccharide induces TREM-1-dependent HIF-1α expression in human keratinocyte cell line. Cell Biol Int. 2016 Oct 15;: Authors: Tu C, Wang S, Hu X, Wang W, Dong Y, Xiao S, Wang X Abstract Bacterial infection is an important factor that can trigger and exacerbate psoriasis. The protein triggering receptor expressed on myeloid cells type-1 (TREM-1) is overexpressed in psoriasis and decreased after a successful treatment. Hypoxia inducible factor-1α (HIF-1α), subunit of the transcription factor HIF-1, has participated in angiogenesis and inflammation in psoriasis. Increased expressions of TREM-1 ...
Source: Cell Biology International - October 14, 2016 Category: Cytology Authors: Tu C, Wang S, Hu X, Wang W, Dong Y, Xiao S, Wang X Tags: Cell Biol Int Source Type: research

Nuclear co-repressor (NCoR) is required to maintain insulin sensitivity in C2 C12 myotubes.
Abstract Nuclear co-repressor (NCoR) regulate peripheral insulin sensitivity, however its role in modulating insulin sensitivity in skeletal muscle remains elusive. Present study investigated protein expression and effect of NCoR on insulin sensitivity in murine skeletal muscle cell line C2 C12 . Myotubes as compared to myoblasts of C2 C12 cells was found to be more sensitive in response to insulin as increase in insulin stimulated phosphorylation of AKT at serine 473 residue (pAKT(S473) ) was significantly higher in myotubes. Incidentally, reduced protein level of NCoR coincided with differentiation of myoblasts ...
Source: Cell Biology International - December 8, 2016 Category: Cytology Authors: Choudhary AK, Dey CS Tags: Cell Biol Int Source Type: research

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