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Source: Cell Biology International
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Total 159 results found since Jan 2013.
Calycosin suppresses colorectal cancer progression by targeting ER β, upregulating PTEN, and inhibiting PI3K/Akt signal pathway
In this study, we analyzed the effect of calycosin on the viability and apoptosis of human CRC HCT116 and SW480 cells via MTT assay, flow cytometry assay, and caspase-3/7 activity assay. The protein expressions of estrogen receptor β (ERβ), PTEN, and PI3K/Akt signal pathways were determined by Western blot analysis. And then, the alterations of biological behavior in CRC cells transfected with ERβ siRNA were analyzed. Mouse xenograft models were further performed to detect the antitumor effect in vivo. The results show that calycosin reduces CRC cell viability, induces cell apoptosis, and suppresses xenograft tumor grow...
Source: Cell Biology International - July 17, 2022 Category: Cytology Authors: Lin Zhu Sha Liu Yi-Fan Liao Yan-Mei Sheng Ji-Chun He Zheng-Xue Cai Qiong Man Yi-Ying Wu Source Type: research
HPV E6 promotes cell proliferation of cervical cancer cell by accelerating accumulation of RBM15 dependently of autophagy inhibition
This study explored the role of methyltransferase components in HPV-related cervical cancer and the mechanism. The levels of methyltransferase components and autophagy, ubiquitylation of RBM15 protein and the co-localization of lysosomal markers LAMP2A and RBM15 were measured. CCK-8 assay, flow cytometry, clone formation experiment and immunofluorescence assay were conducted to measure cell proliferation. The mouse tumor model was developed to study the cell growth in vivo. The binding of RBM15 to c-myc mRNA and m6A modifcation of c-myc mRNA were analyzed. The expressions of METTL3, RBM15 and WTAP were higher in HPV-positi...
Source: Cell Biology International - May 16, 2023 Category: Cytology Authors: Gang Nie Bo Tang Mingfen Lv Danyang Li Tian Li Rongying Ou Yunsheng Xu Juan Wen Source Type: research
Role of 14-3-3σ in resistance to cisplatin in non-small cell lung cancer cells.
Abstract
The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 and Calu1/CR-Calu1) and clinical lung cancer biopsy samples were analysed for 14-3-3σ expression. Cell viability was assessed by WST assay; and 14-3-3σ expression was suppressed by siRNA transfection. 14-3-3σ mRNA expression increased in CR-A549 and CR-Calu1 compared with their cisplatin-se...
Source: Cell Biology International - January 1, 2013 Category: Cytology Authors: Cetintas VB, Tetik A, Cok G, Kucukaslan AS, Kosova B, Gunduz C, Veral A, Eroglu Z Tags: Cell Biol Int Source Type: research
Expression and biological function of programmed death ligands in human placenta mesenchymal stem cells.
Abstract
Mesenchymal stem cells (MSCs) play important roles in tissue regeneration due to their self-renewal, multilineage differentiation and immunosuppression abilities. MSCs can be isolated from various kinds of tissue, such as umbilical cord, cord blood and placenta. Human placenta mesenchymal stem cells (hPMSCs) possess stronger immunosuppressive properties, such as the ability to inhibit T-cell activation and proliferation, than human bone marrow MSCs. We have investigated that the roles of the programmed death ligands 1 and 2 (PDL1 and PDL2) in hPMSC adhesion, migration and immunosuppression were investigat...
Source: Cell Biology International - January 30, 2013 Category: Cytology Authors: Wang G, Zhang S, Wang F, Li G, Zhang L, Luan X Tags: Cell Biol Int Source Type: research
Mycophenolic acid regulates spleen tyrosine kinase to repress tumour necrosis factor-alpha-induced monocyte chemotatic protein-1 production in cultured human aortic endothelial cells.
Abstract
Atherosclerosis develops from cascades of inflammatory processes. Spleen tyrosine kinase (Syk) and monocyte chemotatic protein-1 (MCP-1) play important roles in the pathogenesis of atherosclerosis. Mycophenolic acid (MPA) has an anti-inflammatory effect. We have investigated whether MPA regulates Syk to repress tumour necrosis factor-α (TNF-α)-induced MCP-1 production in cultured human aortic endothelial cells. Expression of MCP-1 mRNA and its protein were measured by real time RT-PCR and ELISA, respectively. Reactive oxygen species (ROS) production were measured using 2'7'-dichlorofluorescein diacetate...
Source: Cell Biology International - January 1, 2013 Category: Cytology Authors: Koo TY, Kim YJ, Yang WS, Park JS, Han NJ, Lee JM, Park SK Tags: Cell Biol Int Source Type: research
Aurora B kinase is required for cytokinesis through effecting spindle structure.
Abstract
The spindle, composed of microtubules and associated proteins, serves as major machinery for cell division. Aurora B is a chromosome passenger protein that has important functions in mitosis. Its dynamic distribution at mitosis goes along with spindle structure and dynamics. We used the siRNA technique to knockdown protein expression and immunofluorescence technique to follow Aurora B during mitosis. Aurora B regulates microtubule plus ends as mitosis progresses, including both kinetochore and polar microtubules. Interactions between Aurora B and polar microtubule plus ends lead to failure in cytokinesis ...
Source: Cell Biology International - May 1, 2013 Category: Cytology Authors: Wu Q, Zhang W, Mu T, Song T, Li D Tags: Cell Biol Int Source Type: research
TGF-β1 induces a nucleus pulposus-like phenotype in Notch 1 knockdown rabbit bone marrow mesenchymal stem cells.
Abstract
We have investigated the effects of Notch1 knockdown and treatment with TGF-β1 on the regulation of the directional differentiation of mesenchymal stem cells (MSCs). MSCs were isolated from the femur bone of New Zealand rabbits and purified by using discontinuous gradient density centrifugation. Notch1 siRNAs were designed, synthesised and transiently transfected into these MSCs, and treated with TGF-β1. The MSCs were examined for morphology, stained with toluidine blue for proteoglycan analysis and gene and protein expression were measured with qRT-PCR and Western blotting respectively. They had an ell...
Source: Cell Biology International - March 30, 2013 Category: Cytology Authors: Morigele M, Shao Z, Zhang Z, Kaige M, Zhang Y, Qiang W, Yang S Tags: Cell Biol Int Source Type: research
Regulation of chondrocyte proliferation through GIT1-Rac1-mediated ERK1/2 pathway by PDGF.
Abstract
There are many growth factors contributing to fracture healing after bone fractures. Platelet-derived growth factor (PDGF) released from platelets is a factor promoting cell division and proliferation, and first appears around the sites of fractures. Culture of chondrocytes in vitro are stimulated by PDGF to proliferation, its presence being upregulated in the extracellular matrix of cartilage; the main components include aggrecan and type Ⅱ collagen. PDGF induces the expression of G the protein-coupled receptor kinase interacting protein 1 (GIT1), promoting Rac1 and ERK1/2 phosphorylation. Both knockin...
Source: Cell Biology International - January 13, 2014 Category: Cytology Authors: Xiao J, Chen X, Xu L, Zhang Y, Yin Q, Wang F Tags: Cell Biol Int Source Type: research
IL-8 enhances angiogenesis of human bone marrow mesenchymal stem cells by increasing vascular endothelial growth factor.
In conclusion, IL-8 stimulates VEGF production in hBM-MSCs in part via the PI3K/Akt and MAPK/ERK signal transduction pathways and that administration of IL-8-treated hBM-MSCs increases angiogenesis after stroke. This approach may be used to optimize MSC-based therapies for numerous diseases including stroke, myocardial ischemia, and spinal cord injury.
PMID: 24797366 [PubMed - as supplied by publisher]
Source: Cell Biology International - May 5, 2014 Category: Cytology Authors: Hou Y, Ryu CH, Jun JA, Kim SM, Jeong CH, Jeun SS Tags: Cell Biol Int Source Type: research
