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Total 409 results found since Jan 2013.
Intravitreal allogeneic mesenchymal stem cells: a non-randomized phase II clinical trial for acute non-arteritic optic neuropathy
CONCLUSIONS: Besides the development of an epiretinal membrane in one patient, the intravitreal application of allogeneic BM-MSCs appeared to be intraocularly well tolerated. Consequently, not only NA-AION but also BM-MSCs deserve more clinical investigational resources and a larger randomized multicenter trial that would provide stronger evidence both about safety and the potential therapeutic efficacy of intravitreally injected allogeneic BM-MSCs in acute NA-AION.TRIAL REGISTRATION: Safety Assessment of Intravitreal Mesenchymal Stem Cells for Acute Non-Arteritic Anterior Ischemic Optic Neuropathy (NEUROSTEM). NCT03173638...
Source: Cell Research - September 22, 2023 Category: Cytology Authors: Jose C Pastor Salvador Pastor-Idoate Marina L ópez-Paniagua Marta Para Francisco Blazquez Esther Murgui Ver ónica García Rosa M Coco-Mart ín Source Type: research
A novel primary porcine retinal pigment epithelium cell model with preserved properties
CONCLUSIONS: This work describes, for the first time, a pRPE cell model that exhibits preserved RPE properties for several passages on cell culture plastic plates. Though RPE characteristics were maintained for at least 6 passages, the reduced CRALBP and RPE-65 with passaging emphasize that lower passage cells are advantageous to utilise, and that morphology, barrier function and ZO-1 localisation cannot be solely employed as a quality measure of RPE identity. Pigs are phylogenetically similar to humans, including similar physiology, anatomy and immune system. Therefore, porcine RPE cells constitute a relevant model system...
Source: Cell Research - September 19, 2023 Category: Cytology Authors: Dinna Zhou Anne Petersen Julia Adel öf Malin Hernebring Madeleine Zetterberg Source Type: research
Unleashing the potential of CRISPR multiplexing: Harnessing Cas12 and Cas13 for precise gene modulation in eye diseases
Vision Res. 2023 Sep 16;213:108317. doi: 10.1016/j.visres.2023.108317. Online ahead of print.ABSTRACTGene therapy is a flourishing field with the potential to revolutionize the treatment of genetic diseases. The emergence of CRISPR-Cas9 has significantly advanced targeted and efficient genome editing. Although CRISPR-Cas9 has demonstrated promising potential applications in various genetic disorders, it faces limitations in simultaneously targeting multiple genes. Novel CRISPR systems, such as Cas12 and Cas13, have been developed to overcome these challenges, enabling multiplexing and providing unique advantages. Cas13, in...
Source: Cell Research - September 18, 2023 Category: Cytology Authors: Fabio Bigini Soo Hyeon Lee Young Joo Sun Yang Sun Vinit B Mahajan Source Type: research
PRPF31-retinitis pigmentosa: Challenges and opportunities for clinical translation
Vision Res. 2023 Sep 13;213:108315. doi: 10.1016/j.visres.2023.108315. Online ahead of print.ABSTRACTMutations in pre-mRNA processing factor 31 cause autosomal dominant retinitis pigmentosa (PRPF31-RP), for which there is currently no efficient treatment, making this disease a prime target for the development of novel therapeutic strategies. PRPF31-RP exhibits incomplete penetrance due to haploinsufficiency, in which reduced levels of gene expression from the mutated allele result in disease. A variety of model systems have been used in the investigation of disease etiology and therapy development. In this review, we discu...
Source: Cell Research - September 15, 2023 Category: Cytology Authors: Hamzah Aweidah Zhouhuan Xi Jos é-Alain Sahel Leah C Byrne Source Type: research
Suprachoroidal spheroidal mesenchymal stem cell implantation in retinitis pigmentosa: clinical results of 6 months follow-up
CONCLUSION: Suprachoroidal implantation of MSCs in the form of spheroids as a stem cell therapy for RP patients with relatively good visual acuity has an improving effect on BCVA, VF and mfERG recordings during the 6-month follow-up period. Spheroidal MSCs with enhanced effects may be more successful in preventing apoptosis and improving retinal tissue healing in RP patients.PMID:37705097 | DOI:10.1186/s13287-023-03489-z
Source: Cell Research - September 13, 2023 Category: Cytology Authors: Berna Özkan B üşra Yılmaz Tuğan Cansu Hem şinlioğlu G özde Sır Karakuş Özlem Şahin Erc üment Ovalı Source Type: research
M üller cell vulnerability in aging human retina: Implications on photoreceptor cell survival
This study examined age-related changes in MC of donor human retina (age: 35-98 years; N = 18 donors). Ultrastructural and immunohistochemical observations indicate that MC undergo gliosis and increased lipid peroxidation, and show osmotic changes with advanced aging (>80 years). Photoreceptor cells also undergo oxidative-nitrosative stress with aging, and their synapses also show clear osmotic swelling. MC respond to oxidative stress via proliferation of smooth endoplasmic reticulum in their processes, and increased expression of aquaporin-4 in endfeet and outer retina. In advanced aged retinas (81-98 years), they show...
Source: Cell Research - September 8, 2023 Category: Cytology Authors: Tapas C Nag Source Type: research
Characterization of Central and Nasal Orbital Adipose Stem Cells and their Neural Differentiation Footprints
CONCLUSION: We suggested the explant method of culture as an easy and suitable method for the expansion of OASCs. Our findings denote mesenchymal properties of both central and nasal OASCs, while mesenchymal and neural characteristics were expressed stronger in nasal OASCs when compared to central ones. These findings can be added to the literature when cell transplantation is targeted in the treatment of neuro-retinal degenerative disorders.PMID:37670706 | DOI:10.2174/1574888X19666230905114246
Source: Cell Research - September 6, 2023 Category: Cytology Authors: Fatemeh Sanie-Jahromi M Hossein Nowroozzadeh Mina Shaabanian Behzad Khademi Naser Owji Davood Mehrabani Source Type: research
A simple, rapid fluorescent reporter-based method for detection of ectopic cre recombinase expression in presumed retinal cell type-targeted mouse lines
Exp Eye Res. 2023 Aug 31:109637. doi: 10.1016/j.exer.2023.109637. Online ahead of print.ABSTRACTAlthough cell type-specific Cre recombinase-expressing mouse lines are commonly used to generate conditional knockout of genes of interest, germline recombination and ectopic "leakiness" in Cre recombinase expression in non-specific cell types has been observed in several neuronal and glial-specific Cre lines. This often leads to inadvertent loss of conditional mouse lines, requiring rederivation. It is therefore imperative to be able to monitor and validate cell type-specific Cre recombinase-mediated gene editing. Herein, we de...
Source: Cell Research - September 2, 2023 Category: Cytology Authors: Sriganesh Ramachandra Rao Steven J Fliesler Source Type: research
Intercellular communication atlas reveals Oprm1 as a neuroprotective factor for retinal ganglion cells
Res Sq. 2023 Aug 17:rs.3.rs-3193738. doi: 10.21203/rs.3.rs-3193738/v1. Preprint.ABSTRACTThe progressive death of mature neurons often results in neurodegenerative diseases. While the previous studies have mostly focused on identifying intrinsic mechanisms controlling neuronal survival, the extracellular environment also plays a critical role in regulating cell viability. Here we explore how intercellular communication contributes to the survival of retinal ganglion cells (RGCs) following the optic nerve crush (ONC). Although the direct effect of the ONC is restricted to the RGCs, we observed transcriptomic responses in oth...
Source: Cell Research - August 30, 2023 Category: Cytology Authors: Jiang Qian Cheng Qian Ying Xin Cheng Qi Hui Wang Donald Zack Seth Blackshaw Samer Hattar Feng-Quan Zhou Source Type: research
How the immune response to the structural proteins of SARS-CoV-2 affects the retinal vascular endothelial cells: an immune thrombotic and/or endotheliopathy process with in silico modeling
Immunol Res. 2023 Aug 29. doi: 10.1007/s12026-023-09412-1. Online ahead of print.ABSTRACTThrombotic events associated with SARS-CoV-2 at the vascular endothelium still remains unclear. The aim of the current study is to determine the relationship between cellular proteins on the (ocular) vascular endothelial surface and the immune thrombotic and/or endotheliopathy process elicited by SARS-CoV-2 using an in-silico modeling. The structural S (spike glycoprotein), N (nucleocapsid protein), M (membrane protein), and E (envelope protein) proteins, an accessory protein (ORF1ab) of SARS-CoV-2 and 158 cellular proteins associated ...
Source: Cell Research - August 29, 2023 Category: Cytology Authors: I şıl Kutlutürk Ecem Önder Tokuç Levent Karaba ş Ren é Rückert M ücahit Kaya Ali Karag öz Marion R Munk Source Type: research
Metabolic changes and retinal remodeling in Heterozygous CRX mutant cats (CRX < sup > RDY/+ < /sup > )
This study investigated retinal remodeling that occurs as photoreceptors degenerate. CRXRdy/+ cats from 6 weeks to 10 years of age were investigated. In vivo structural changes of retinas were analyzed by fundus examination, confocal scanning laser ophthalmoscopy and spectral domain optical coherence tomography. Histologic analyses including immunohistochemistry for computational molecular phenotyping with macromolecules and small molecules. Affected cats had a cone-led photoreceptor degeneration starting in the area centralis. Initially there was preservation of inner retinal cells such as bipolar, amacrine and horizontal...
Source: Cell Research - August 25, 2023 Category: Cytology Authors: Laurence M Occelli Bryan W Jones Taylor J Cervantes Simon M Petersen-Jones Source Type: research
Modeling subcellular specificity in the developing retina
Res Sq. 2023 Aug 8:rs.3.rs-3214285. doi: 10.21203/rs.3.rs-3214285/v1. Preprint.ABSTRACTThe precise wiring of the nervous system relies on neurons extending their processes at the right time and place to find their appropriate synaptic partner. The mechanisms that determine when and where neurons extend their neurites during synaptogenesis remains a central question in the field. In the present study, we developed a cell culture system coupled with live imaging to investigate the wiring mechanisms in the developing nervous system. We focused on horizontal cells which are interneurons in the mammalian outer retina known to s...
Source: Cell Research - August 23, 2023 Category: Cytology Authors: Ross Perez Yong Park Arlene Hirano Nicholas Brecha Benjamin Frankfort Elizabeth Zuniga-Sanchez Source Type: research
Soluble CX3CL1-expressing retinal pigment epithelium cells protect rod photoreceptors in a mouse model of retinitis pigmentosa
CONCLUSIONS: Transgenic hESCs differentiate into hRPE cells and retain sCX3CL1 transgene expression both in vitro and in vivo. Moreover, hRPE cells delivered to the subretinal space of rd10 mice prevented photoreceptor degeneration in a local-acting manner, suggesting that this approach could have applications for preserving photoreceptors in specific subregions of the retina, such as the macula. Overall, our study not only reveals the potential of a combined cell and gene therapy for the treatment of RP, but also the possibility of using hRPE cells to deliver therapeutic biologics in situ to treat diseases over long-term....
Source: Cell Research - August 22, 2023 Category: Cytology Authors: Eric D Jong Sabiha Hacibekiroglu Lily Guo Evan Sawula Biao Li Chengjin Li Margaret T Ho Molly S Shoichet Valerie A Wallace Andras Nagy Source Type: research
Decreased retinal and choroidal endothelial surface molecules in spontaneously hypertensive rats
In this study, we have examined changes in protein expression of several molecules including platelet endothelial cell adhesion molecule-1 (PECAM-1), vascular endothelial cadherin (VE-cadherin), glypican-1, and syndecan-1, in spontaneously hypertensive rats (SHR) compared to control normotensive Wistar Kyoto (WKY) rats. In male SHR vs WKY rat retinas, decreases were found for VE-cadherin and syndecan-1; whereas in female retinas, decreases were found for PECAM-1, glypican-1, and syndecan-1. In male SHR vs WKY rat choroid, we found an increase in glypican-1, but choroidal syndecan-1 was decreased in SHR in both males and fe...
Source: Cell Research - August 18, 2023 Category: Cytology Authors: Randa S Eshaq Minsup Lee Wendy Leskova Norman R Harris Source Type: research
Knockout of Sema4D alleviates liver fibrosis by suppressing AOX1 expression
Pharmacol Res. 2023 Aug 15:106886. doi: 10.1016/j.phrs.2023.106886. Online ahead of print.ABSTRACTLiver fibrosis can occur in many chronic liver diseases, and no effective treatments are available due to the poorly characterized molecular pathogenesis. Semaphorin 4D (Sema4D) has immune functions and serves important roles in T cell priming. Here, we found that Sema4D was highly expressed in fibrotic liver, and the expression of Sema4D increased with hepatic stellate cells (HSCs) activation. Knockout of Sema4D alleviated liver fibrosis. Mechanistically, knockout of Sema4D alleviated liver fibrosis by suppressing the express...
Source: Cell Research - August 17, 2023 Category: Cytology Authors: Lifu Wang Dinghao Li Zifeng Zhu Yao Liao Ji Wu Yuheng Liu Ruibing Yang Hanqiao Dai Zhongdao Wu Xi Sun Source Type: research
