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Source: Journal of Forensic Sciences

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Total 27 results found since Jan 2013.

In Vitro Effects of Household Products on Calliphoridae Larvae Development: Implication for Forensic Entomology.
This study focuses on the development of Lucilia sericata (Diptera: Calliphoridae) (Meigen, 1826) on household chemical-contaminated substrates. bleach, perfume, hydrochloric acid, caustic soda, insecticide, mosquito repellent, and gasoline in quantities consistent with an amount that could possibly be spilled on a corpse were mixed with beef liver to simulate contaminated fleshes. Larvae were bred at 25°C on these media until emergence. Four developmental parameters were followed: survival rates, development times, sex ratios, and adult sizes. Hydrochloric acid, insecticide, and gasoline killed all larvae. In low quantit...
Source: Journal of Forensic Sciences - July 26, 2014 Category: Forensic Medicine Authors: Aubernon C, Devigne C, Hedouin V, Gosset D, Charabidze D Tags: J Forensic Sci Source Type: research

Blow Flies from Forest Fragments Embedded in Different Land Uses: Implications for Selecting Indicators in Forensic Entomology.
This study was conducted in 17 fragments across different landscapes in central Brazil. Different land cover type proportions were used to represent landscape structure. In total, 2334 specimens of nine species of Calliphoridae were collected. We used principal component analysis (PCA) to reduce dimensionality and multicollinearity of the landscape data. The first component explained 70%, and it represented a gradient of forest-pasture land uses. Alien species showed a wide distribution in different fragments with no clear relationship between the abundance values and the scores of PCA axes, whereas native species occurred...
Source: Journal of Forensic Sciences - August 6, 2015 Category: Forensic Medicine Authors: de Souza MS, Pepinelli M, de Almeida EC, Ochoa-Quintero JM, Roque FO Tags: J Forensic Sci Source Type: research

DNA Barcoding in Forensic Entomology - Establishing a DNA Reference Library of Potentially Forensic Relevant Arthropod Species.
Abstract Throughout the years, DNA barcoding has gained in importance in forensic entomology as it leads to fast and reliable species determination. High-quality results, however, can only be achieved with a comprehensive DNA barcode reference database at hand. In collaboration with the Bavarian State Criminal Police Office, we have initiated at the Bavarian State Collection of Zoology the establishment of a reference library containing arthropods of potential forensic relevance to be used for DNA barcoding applications. CO1-5P' DNA barcode sequences of hundreds of arthropods were obtained via DNA extraction, PCR ...
Source: Journal of Forensic Sciences - July 11, 2018 Category: Forensic Medicine Authors: Chimeno C, Morinière J, Podhorna J, Hardulak L, Hausmann A, Reckel F, Grunwald JE, Penning R, Haszprunar G Tags: J Forensic Sci Source Type: research

Correlation of Molecular Expression with Diel Rhythm of Oviposition in Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) and Implications for Forensic Entomology.
This study explored the molecular mechanisms potentially underlying blow fly nocturnal oviposition. A behavioral study revealed that Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) possesses a diel rhythm of oviposition in light under 12:12 light/dark conditions. Reversal to 12:12 dark/light resulted in oviposition behavior changing to align with the adjusted regime in most females, but four of 59 experimental females lacked a diel rhythm of oviposition (were arrhythmic). Real-time PCR was used to monitor the molecular expression levels of known circadian genes per and tim in C. vicina to determine whether ...
Source: Journal of Forensic Sciences - August 3, 2014 Category: Forensic Medicine Authors: George KA, Archer MS, Toop T Tags: J Forensic Sci Source Type: research

Forensic Entomology and the Estimation of the Minimum Time Since Death in Indoor Cases.
Abstract Eight cases that occurred indoors in which the insects played an important role in the mPMI estimation are presented. The bodies of socially isolated people and old people living alone were discovered in central Italy between June and November. mPMI ranged from a few days to several weeks. Insects were collected during the body recovery and the postmortem. Climatic data were obtained from the closest meteorological stations and from measurements performed on the site. Sarcophagidae and Calliphoridae species were present in 75% of the cases with Lucilia sericata and Chrysomya albiceps collected in 50% of t...
Source: Journal of Forensic Sciences - November 14, 2014 Category: Forensic Medicine Authors: Bugelli V, Forni D, Bassi LA, Di Paolo M, Marra D, Lenzi S, Toni C, Giusiani M, Domenici R, Gherardi M, Vanin S Tags: J Forensic Sci Source Type: research

The Effect of Flunitrazepam (Rohypnol(®) ) on the Development of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) and its Implications for Forensic Entomology.
This study investigated the potential effects of flunitrazepam (known as "date rape drug") on the developmental cycle of Chrysomya megacephala, an important forensic species, and their possible implications for the calculation of the PMI. A 1050 C. megacephala eggs were divided into five groups with seven replications each. The eggs were placed on artificial diet prepared with four drug concentrations of flunitrazepam (4, 8, 16, and 32 ng/g), besides the control group (prepared with water). Were evaluated the potential effects on development time, weight gain, and mortality during the cycles. The drug had no significant ...
Source: Journal of Forensic Sciences - May 2, 2016 Category: Forensic Medicine Authors: Baia TC, Campos A, Wanderley BM, Gama RA Tags: J Forensic Sci Source Type: research

Context Effects in Forensic Entomology and Use of Sequential Unmasking in Casework.
Abstract Context effects are pervasive in forensic science, and are being recognized by a growing number of disciplines as a threat to objectivity. Cognitive processes can be affected by extraneous context information, and many proactive scientists are therefore introducing context-minimizing systems into their laboratories. Forensic entomologists are also subject to context effects, both in the processes they undertake (e.g., evidence collection) and decisions they make (e.g., whether an invertebrate taxon is found in a certain geographic area). We stratify the risk of bias into low, medium, and high for the deci...
Source: Journal of Forensic Sciences - July 4, 2016 Category: Forensic Medicine Authors: Archer MS, Wallman JF Tags: J Forensic Sci Source Type: research

Developmental Times of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) at Constant Temperatures and Applications in Forensic Entomology.
Abstract The characteristic life stages of infesting blowflies (Calliphoridae) such as Chrysomya megacephala (Fabricius) are powerful evidence for estimating the death time of a corpse, but an established reference of developmental times for local blowfly species is required. We determined the developmental rates of C. megacephala from southwest China at seven constant temperatures (16-34°C). Isomegalen and isomorphen diagrams were constructed based on the larval length and time for each developmental event (first ecdysis, second ecdysis, wandering, pupariation, and eclosion), at each temperature. A thermal summa...
Source: Journal of Forensic Sciences - August 31, 2016 Category: Forensic Medicine Authors: Yang YQ, Li XB, Shao RY, Lyu Z, Li HW, Li GP, Xu LZ, Wan LH Tags: J Forensic Sci Source Type: research

Commentary on: Chimeno C, Morini ère J, Podhorna J, Hardulak, L, Hausmann A, Reckel F, et al. DNA barcoding in forensic entomology-establishing a DNA reference library of potentially forensic relevant arthropod species. J Forensic Sci 2019;64(2):593-601.
Commentary on: Chimeno C, Morinière J, Podhorna J, Hardulak, L, Hausmann A, Reckel F, et al. DNA barcoding in forensic entomology-establishing a DNA reference library of potentially forensic relevant arthropod species. J Forensic Sci 2019;64(2):593-601. J Forensic Sci. 2019 May 31;: Authors: Kotrba M PMID: 31150116 [PubMed - as supplied by publisher]
Source: Journal of Forensic Sciences - May 30, 2019 Category: Forensic Medicine Authors: Kotrba M Tags: J Forensic Sci Source Type: research

An Evaluation of Differentially Spliced Genes as Markers of Sex for Forensic Entomology*, †, ‡.
Abstract Blow flies (Calliphoridae) are important medically and economically and are commonly used in forensics as temporal markers in death investigations. While phenotypic traits in adult flies can be sexually dimorphic, sex identification in immatures is difficult. Consequently, little is known about how sex may result in developmental disparities among sexes even though there are indications that they may be important in some instances. Since genetic mechanisms for sex are well studied in model flies and species of agricultural and medical importance, we exploit the sex-specifically spliced genes transformer (...
Source: Journal of Forensic Sciences - June 4, 2020 Category: Forensic Medicine Authors: Jonika MM, Hjelmen CE, Faris AM, McGuane AS, Tarone AM Tags: J Forensic Sci Source Type: research

Infrared spectroscopy and forensic entomology: Can this union work? A literature review
J Forensic Sci. 2021 Jul 22. doi: 10.1111/1556-4029.14800. Online ahead of print.ABSTRACTFor more than two decades, infrared spectroscopy techniques combined with multivariate analysis have been efficiently applied in several entomological fields, such as Taxonomy and Toxicology. However, little is known about its use and applicability in Forensic entomology (FE) field, with vibrational techniques such as Near-infrared spectroscopy (NIRS) and Medium-infrared spectroscopy (MIRS) underutilized in forensic sciences. Thus, this work describes the potential of NIRS, MIRS, and other spectroscopic methodologies, for entomological...
Source: Journal of Forensic Sciences - July 22, 2021 Category: Forensic Medicine Authors: Jessica T Jales Taciano M Barbosa Juc élia R de Medeiros Leomir A S de Lima K ássio M G de Lima Renata A Gama Source Type: research

First report of the forensically important fly, Stearibia nigriceps (Diptera: Piophilidae) in South Korea: Confirmation of specimens from human corpses based on cytochrome c oxidase subunit I barcodes
J Forensic Sci. 2021 Apr 26. doi: 10.1111/1556-4029.14721. Online ahead of print.ABSTRACTPiophilidae are a relatively small family of Diptera that is frequently associated with cadavers at advanced stages of decomposition and are, therefore, considered potentially useful forensic indicators. However, their use in forensic investigations is typically hampered by a deficiency in reliable identification tools. This is particularly evident in countries such as South Korea, where forensic entomology is still in its infancy and the diversity of forensically relevant insect taxa remains largely undocumented. In the present study,...
Source: Journal of Forensic Sciences - April 26, 2021 Category: Forensic Medicine Authors: Hajin Kim Daniel Mart ín-Vega Sang Eon Shin Ah Rha Wang Seong Hwan Park Source Type: research

Diversity of Forensic Rove Beetles (Coleoptera, Staphylinidae) Associated with Decaying Pig Carcass in a Forest Biotope.
Abstract Most forensic studies are focused on Diptera pattern colonization while neglecting Coleoptera succession. So far, little information is available on the postmortem colonization by beetles and the decomposition process they initiate under temperate biogeoclimatic countries. These beetles have, however, been referred to as being part of the entomofaunal colonization of a dead body. Forensic entomologists need increased databases detailing the distribution, ecology, and phenology of necrophagous insects, including staphylinids (Coleoptera, Staphylinidae). While pig carcasses are commonly used in forensic ent...
Source: Journal of Forensic Sciences - March 28, 2013 Category: Forensic Medicine Authors: Dekeirsschieter J, Frederick C, Verheggen FJ, Drugmand D, Haubruge E Tags: J Forensic Sci Source Type: research