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Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research
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Total 33 results found since Jan 2013.
Nerve/glial antigen (NG) 2 is a crucial regulator of intercellular adhesion molecule (ICAM)-1 expression
In this study, we demonstrate that in pericytes and glioblastoma cells intercellular adhesion molecule (ICAM)-1, an essential protein for leukocyte adhesion and transmigration, underlies a NG2-dependent expression. As shown by flow cytometry, Western blot analysis and quantitative real-time polymerase chain reaction (qRT-PCR), silencing of NG2 in human placenta-derived pericytes increased the expression of ICAM-1. Pathway analyses revealed that this is mediated by extracellular-regulated-kinases (ERK) 1/2 signaling. Moreover, leukocyte adhesion to NG2 siRNA-treated pericytes was significantly enhanced when compared to scra...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research
NADPH oxidase subunit p22phox-mediated reactive oxygen species contribute to angiogenesis and tumor growth through AKT and ERK1/2 signaling pathways in prostate cancer
In this study, we reported that levels of ROS and p22phox expression are greatly increased in human prostate cancer tissues, and knockdown of p22phox by specific small interfering RNA (siRNA) decreased ROS levels in prostate cancer cells. We also showed that stable downregulation of p22phox in prostate cancer cells inhibited cell proliferation and colony formation, which was mediated by AKT and extracellular signal-regulated kinase (ERK)1/2 signaling pathways and their downstream molecules hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF). The NADPH oxidase subunit NOX1 was also elevated ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 1, 2014 Category: Molecular Biology Source Type: research
Involvement of store-operated Ca2+ entry in activation of AMP-activated protein kinase and stimulation of glucose uptake by M3 muscarinic acetylcholine receptors in human neuroblastoma cells
Publication date: December 2014 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1843, Issue 12 Author(s): Maria C. Olianas , Simona Dedoni , Pierluigi Onali Gq/11-coupled muscarinic acetylcholine receptors (mAChRs) belonging to M1, M3 and M5 subtypes have been shown to activate the metabolic sensor AMP-activated protein kinase (AMPK) through Ca2+/calmodulin-dependent protein kinase kinase-β (CaMKKβ)-mediated phosphorylation at Thr172. However, the source of Ca2+ required for this response has not been yet elucidated. Here, we investigated the involvement of store-operated Ca2+ entry (SOCE) ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 11, 2014 Category: Molecular Biology Source Type: research
Novel role for TRPC4 in regulation of macroautophagy by a small molecule in vascular endothelial cells
In this study, we identified trans-3,5,4′-trimethoxystilbene (TMS) as a potent small molecule inducer of autophagy in human umbilical vascular endothelial cells (HUVECs) in the presence of serum. Using high-throughput DNA microarray and siRNA transfection technologies, we demonstrated that TMS induced autophagy by up-regulating the expression of the transient receptor potential canonical channel 4 (TRPC4), an important cation channel in HUVECs. In addition, the overexpression of TRPC4 by plasmid transfection also induced autophagy. Mechanistic studies revealed that the up-regulation of TRPC4 increased the intracellular C...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2014 Category: Molecular Biology Source Type: research
Downregulation of diacylglycerol kinase ζ enhances activation of cytokine-induced NF-κB signaling pathway
This study shows that siRNA-mediated DGKζ knockdown in HeLa cells facilitates degradation of IκB, followed by nuclear translocation of NF-κB p65 subunit. In addition, DGKζ-deficient MEFs show upregulation of p65 subunit phosphorylation at Serine 468 and 536 and its interaction with CBP transcriptional coactivator upon TNF-α stimulation. These modifications of p65 subunit might engender enhanced NF-κB transcriptional reporter assay of DGKζ knockdown cells. These findings provide further insight into the regulatory mechanisms of cytokine-induced NF-κB activation.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 5, 2014 Category: Molecular Biology Source Type: research
CSN-associated USP48 confers stability to nuclear NF-κB/RelA by trimming K48-linked Ub-chains
In this study we observed that USP48, a DUB of hitherto ill-defined function identified through a siRNA screen, associates with the CSN and RelA in the nucleus. We show that USP48 trims rather than completely disassembles long K48-linked free and substrate-anchored Ub-chains, a catalytic property only shared with ataxin-3 (Atx3) and otubain-1 (OTU1), and that USP48 Ub-chain-trimming activity is regulated by casein-kinase-2 (CK2)-mediated phosphorylation in response to cytokine-stimulation. Functionally, we demonstrate for the first time the CSN and USP48 to cooperatively stabilize the nuclear pool of RelA, thereby facilita...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 5, 2014 Category: Molecular Biology Source Type: research
Mitochondrial function contributes to oxysterols-induced osteogenic differentiation in mouse embryonic stem cells
This study investigated the effect of an oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol (SS) on osteogenic differentiation of ESCs and the alterations to mitochondrial activity during differentiation. Osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity, matrix mineralization, mRNA expression of osteogenic factors, runt-related transcription factor 2, osterix, and osteocalcin, and protein levels of collagen type IA (COLIA) and osteopontin (OPN). Treatment of cells with SS increased osteoinductive activity compared to the control group. Intracellular reactive oxygen ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 16, 2014 Category: Molecular Biology Source Type: research
Mitochondrial function contributes to oxysterol-induced osteogenic differentiation in mouse embryonic stem cells
This study investigated the effect of an oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol (SS) on osteogenic differentiation of ESCs and the alterations to mitochondrial activity during differentiation. Osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity, matrix mineralization, mRNA expression of osteogenic factors, runt-related transcription factor 2, osterix, and osteocalcin, and protein levels of collagen type IA (COLIA) and osteopontin (OPN). Treatment of cells with SS increased osteoinductive activity compared to the control group. Intracellular reactive oxygen ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 24, 2015 Category: Molecular Biology Source Type: research
Nuclear envelope proteins Nesprin2 and LaminA regulate proliferation and apoptosis of vascular endothelial cells in response to shear stress
Publication date: Available online 23 February 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Yue Han , Lu Wang , Qing-Ping Yao , Ping Zhang , Bo Liu , Guo-Liang Wang , Bao-Rong Shen , Binbin Cheng , Yingxiao Wang , Zong-Lai Jiang , Ying-Xin Qi The dysfunction of vascular endothelial cells (ECs) influenced by flow shear stress is crucial for vascular remodeling. However, the roles of nuclear envelope (NE) proteins in shear stress-induced EC dysfunction are still unknown. Our results indicated that, compared with normal shear stress (NSS), low shear stress (LowSS) suppressed the expre...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 23, 2015 Category: Molecular Biology Source Type: research
Oncostatin M-dependent Mcl-1 induction mediated by JAK1/2-STAT1/3 and CREB contributes to bioenergetic improvements and protective effects against mitochondrial dysfunction in cortical neurons
In conclusion, our findings indicated that OSM induces Mcl-1 expression via activation of ERK1/2, JAK1/2, STAT1/3, and CREB; furthermore, OSM-mediated Mcl-1 induction contributes to bioenergetic improvements and neuroprotective effects against 3-NP toxicity in cortical neurons. OSM may thus serve as a novel neuroprotective agent against mitochondrial dysfunction commonly associated with pathogenic mechanisms underlying neurodegeneration.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - May 16, 2015 Category: Molecular Biology Source Type: research
Dominant negative Ras attenuates pathological ventricular remodeling in pressure overload cardiac hypertrophy
We examined the possible anti-hypertrophic effect of Ras inhibition in vitro using rat neonatal cardiomyocytes (NRCM) and in vivo in the setting of pressure-overload left ventricular (LV) hypertrophy (POH) in rats. Ras functions were modulated via adenovirus directed gene transfer of active mutant Ras-Val12 or dominant negative mutant N17-DN-Ras (DN-Ras). Ras-Val12 expression in vitro activates NFAT resulting in pro-hypertrophic and cardio-toxic effects on NRCM beating and Z-line organization. In contrast, the DN-Ras was antihypertrophic on NRCM, inhibited NFAT and exerted cardio-protective effects attested by preserved NR...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 4, 2015 Category: Molecular Biology Source Type: research
Mitochondrial DNA has a pro-inflammatory role in AMD
This study contributes to our understanding of the potential pro-inflammatory role of mtDNA in the pathogenesis of AMD. Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 7, 2015 Category: Molecular Biology Source Type: research
Tensin1 positively regulates RhoA activity through its interaction with DLC1
Publication date: December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1853, Issue 12 Author(s): Yi-Ping Shih, Peng Sun, Aifeng Wang, Su Hao Lo DLC1 is a RhoGAP-containing tumor suppressor and many of DLC1's functions are absolutely dependent on its RhoGAP activity. Through its RhoGAP domain, DLC1 inhibits the activity of RhoA GTPase, which regulates actin cytoskeleton networks and dis/assembly of focal adhesions. Tensin1 (TNS1) is a focal adhesion molecule that links the actin cytoskeleton to integrins and forms signaling complexes through its multiple binding domains. Here, we ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 4, 2015 Category: Molecular Biology Source Type: research
TRPV6 channel modulates proliferation of insulin secreting INS-1E beta cell line
In conclusion, this study demonstrates for the first time the expression of TRPV6 in INS-1E cells and rat pancreatic beta cells and describes its role in modulating calcium signaling, beta cell proliferation and insulin mRNA expression. In contrast, TRPV6 fails to influence insulin secretion.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 5, 2015 Category: Molecular Biology Source Type: research
Leukotriene B4 receptor-2 contributes to chemoresistance of SK-OV-3 ovarian cancer cells through activation of signal transducer and activator of transcription-3-linked cascade
In this study, we found that the low-affinity leukotriene B4 receptor-2 (BLT2) and its ligand leukotriene B4 were highly up-regulated in cisplatin-resistant SK-OV-3 ovarian cancer cells and play critical roles in mediating the chemoresistance through the activation of signal transducer and activator of transcription-3 (STAT-3) and the subsequent up-regulation of interleukin-6 (IL-6). BLT2 depletion with siRNA clearly abolished the chemoresistance to cisplatin in SK-OV-3 ovarian cancer cells and further increased cell sensitivity to cisplatin chemotherapy by down-regulating the ‘STAT-3-IL-6’ cascade. Enlarged tumor form...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 22, 2015 Category: Molecular Biology Source Type: research
