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Downregulation of nicotinamide N-methyltransferase inhibits migration and epithelial-mesenchymal transition of esophageal squamous cell carcinoma via Wnt/ β-catenin pathway.
Downregulation of nicotinamide N-methyltransferase inhibits migration and epithelial-mesenchymal transition of esophageal squamous cell carcinoma via Wnt/β-catenin pathway. Mol Cell Biochem. 2019 Jul 05;: Authors: Cui Y, Zhang L, Wang W, Ma S, Liu H, Zang X, Zhang Y, Guan F Abstract Nicotinamide N-methyltransferase (NNMT) is an important methyltransferase involved in the biotransformation of many drugs and exogenous compounds. Abnormal expression of NNMT protein is closely associated with the onset and progression of many malignancies, but little is known about its role in esophageal squamous cell ca...
Source: Molecular and Cellular Biochemistry - July 4, 2019 Category: Biochemistry Authors: Cui Y, Zhang L, Wang W, Ma S, Liu H, Zang X, Zhang Y, Guan F Tags: Mol Cell Biochem Source Type: research

Knockdown of differentiation antagonizing non-protein coding RNA exerts anti-tumor effect by up-regulating miR-214 in endometrial carcinoma.
Abstract Differentiation antagonizing non-protein coding RNA (DANCR) is a valuable long noncoding RNA (lncRNA) that involves in the progress of various cancers. However, the functions of DANCR in endometrial carcinoma (EC) have not been validated. In the present study, we aimed to evaluate the roles of DANCR in EC and explore the underlying mechanism. Expression patterns of DANCR in EC specimens and normal control specimens were determined using qRT-PCR. DANCR was knocked down in EC cell lines (AN3CA and HEC-1B) through transfection with small interfering RNA (siRNA) targeting DANCR (si-DANCR). Cell proliferation ...
Source: Molecular and Cellular Biochemistry - June 2, 2019 Category: Biochemistry Authors: Sun J, Gao S, Lu C Tags: Mol Cell Biochem Source Type: research

Glucocorticoids attenuate the sensitivity of glucocorticoid-resistant lymphoid cells to doxorubicin via reduction in OCTN2.
In this study, we examined whether GCs affected the sensitivities to vincristine (VCR)/doxorubicin (DOX) and the expression of drug transporters in GC-resistant cells. The dexamethasone (DEX)/prednisolone (PSL)-resistant lymphoid and non-lymphoid cell lines Raji and HL60 were cultured with DEX for 7 days and then treated with VCR or DOX for 3 days. Seven days of DEX treatment increased the IC50s of both VCR and DOX in Raji cells but not in HL60 cells. The mRNA and protein expression levels of organic cation/carnitine transporter (OCTN) 2, one of the drug uptake transporters expressed in both cell lines, were decreased on...
Source: Molecular and Cellular Biochemistry - May 15, 2019 Category: Biochemistry Authors: Akaihata M, Shikama Y, Matsumoto Y, Ono T, Kimura J, Hosoya M Tags: Mol Cell Biochem Source Type: research

Estrogen-independent role of ER α in ovarian cancer progression induced by leptin/Ob-Rb axis.
In conclusion, our findings demonstrated that ERα, but not ERβ, is involved in leptin-induced ovarian cancer in an E2-independent manner, providing new evidence for cancer progression in obesity-associated ovarian cancer. PMID: 31077012 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - May 9, 2019 Category: Biochemistry Authors: Ghasemi A, Saeidi J, Mohtashami M, Hashemy SI Tags: Mol Cell Biochem Source Type: research

Activation of EMT in colorectal cancer by MTDH/NF- κB p65 pathway.
This study tries to investigate the effect of MTDH gene up-regulation on the activation of EMT in colorectal cancer (CRC) cells and identify the role of NF-κB p65. The CaCO2 cells were divided into three groups: one control group of cultured CaCO2 cells (C1), and two groups of CaCO2 cells co-transfected using human MTDH expression plasmid with either siRNA targeting human NF-κB p65 or its negative control (C2 and C3 respectively). The gene modification was confirmed by qPCR and the effect of gene modification on CRC aggravation was studied. MTDH up-regulation significantly promoted CRC cell proliferation, activated anaer...
Source: Molecular and Cellular Biochemistry - February 28, 2019 Category: Biochemistry Authors: El-Ashmawy NE, El-Zamarany EA, Khedr EG, Abo-Saif MA Tags: Mol Cell Biochem Source Type: research

Heat shock protein 60 negatively regulates the biological functions of ubiquitin-like protein MNSF β in macrophages.
In this study, we purified a 65 kDa MNSFβ adduct from mouse liver lysates by sequential chromatography on DEAE and glutathione S-transferase (GST)-fusioned MNSFβ immobilized on glutathione-Sepharose beads in the presence of ATP. MALDI-TOF mass spectrometry fingerprinting revealed that this MNSFβ adduct consists of an 8.5 kDa MNSFβ and heat shock protein 60 (HSP60), a mitochondrial protein involved in protein folding. Fingerprinting analysis of the MNSFβ adduct demonstrates that MNSFβ conjugates to HSP60 with a linkage between the C-terminal Gly74 and Lys481. HSP60 siRNA neutralized the inhibition of apoptosis by MN...
Source: Molecular and Cellular Biochemistry - February 1, 2019 Category: Biochemistry Authors: Nakamura M, Notsu K, Nakagawa M Tags: Mol Cell Biochem Source Type: research

Up-regulation of PCSK9 gene expression and diminished level of LDL-receptor in rat liver as a potential cause of post-lipectomy hypercholesterolemia.
In conclusion, up-regulation of PCSK9, due to overexpression of HNF1α gene in liver of lipectomized rats and subsequently increase in circulating PCSK9 concentration lead to decrease in liver LDL-R level. This may contribute, at least in part, to an increase in the concentration of circulating cholesterol in rats with reduced fat mass. These findings provide a possible explanation for the molecular mechanism of hypercholesterolemia observed sometimes after reduction of fat mass in human. PMID: 30483910 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - November 27, 2018 Category: Biochemistry Authors: Dettlaff-Pokora A, Sucajtys-Szulc E, Sledzinski T Tags: Mol Cell Biochem Source Type: research

Dopamine promotes cathepsin B-mediated amyloid precursor protein degradation by reactive oxygen species-sensitive mechanism in neuronal cell.
Abstract Recent literature suggested an important function of native amyloid precursor protein (APP) as amine oxidase implicating in protection of brain cells from catecholamine-induced toxicity. However, any role of catecholamines on regulation of native APP has not been explored. Here we report that dopamine (DA), one of the most prominent catecholamine neurotransmitters in brain, down-modulates native APP protein in several neuronal cell types. Using SH-SY5Y cells as model, we detected no alteration of transcript expression and unaffected translation suggested that DA might induce APP degradation. We actually f...
Source: Molecular and Cellular Biochemistry - October 22, 2018 Category: Biochemistry Authors: Kumari S, Mukherjee A, Mukhopadhyay CK Tags: Mol Cell Biochem Source Type: research

HGF promotes HTR-8/SVneo cell migration through activation of MAPK/PKA signaling leading to up-regulation of WNT ligands and integrins that target β-catenin.
Abstract Inadequate migration and invasion of the trophoblast cells during embryo implantation is one of the reasons for pregnancy-related complications such as intrauterine growth restriction and preeclampsia. In the present study, relevance of WNT ligands and integrins associated with hepatocyte growth factor (HGF)-mediated migration of HTR-8/SVneo trophoblastic cells has been investigated. Treatment of HTR-8/SVneo cells with HGF led to a dose-dependent increase in their migration. RT-PCR studies revealed a significant increase in the transcripts of WNT4, WNT11, ITGA2, and ITGAV, which was further confirmed at p...
Source: Molecular and Cellular Biochemistry - August 22, 2018 Category: Biochemistry Authors: Chaudhary P, Malhotra SS, Babu GS, Sobti RC, Gupta SK Tags: Mol Cell Biochem Source Type: research

Comprehensive bioinformation analysis of the miRNA of PLCE1 knockdown in esophageal squamous cell carcinoma.
In this study, PLCE1 siRNA and vector-only plasmid were stably transfected into Eca109 and EC9706 cells and then subjected to miRNA array analysis, and quantitative real-time PCR was applied to validate miRNA array data. Then bioinformatic analyses, such as GO and pathway software, were conducted to obtain data on these differentially expressed miRNAs-targeted genes (DEGs) and clarify their function and pathway. The results showed that 36 miRNAs were found to be differentially expressed in PLCE1 siRNA-transfected cells compared with the control cells. In particular, 28 miRNAs were upregulated while 8 miRNAs were downregula...
Source: Molecular and Cellular Biochemistry - December 13, 2017 Category: Biochemistry Authors: Cui X, Wang K, Yang X, Peng H, Chen X, Xin H, Tian Y, Chen Y, Li F Tags: Mol Cell Biochem Source Type: research

Lipopolysaccharide stimulates endogenous β-glucuronidase via PKC/NF-κB/c-myc signaling cascade: a possible factor in hepatolithiasis formation.
In this study, the mechanism of increased expression and secretion of endogenous β-glucuronidase during hepatolithiasis formation was investigated. We assessed the endogenous β-glucuronidase, c-myc, p-p65, and p-PKC expression in liver specimens with hepatolithiasis by immunohistochemical staining, and found that compared with that in normal liver samples, the expression of endogenous β-glucuronidase, c-myc, p-p65, and p-PKC in liver specimens with hepatolithiasis significantly increased, and their expressions were positively correlated with each other. Lipopolysaccharide (LPS) induced increased expression of endogenous...
Source: Molecular and Cellular Biochemistry - November 29, 2017 Category: Biochemistry Authors: Yao D, Dong Q, Tian Y, Dai C, Wu S Tags: Mol Cell Biochem Source Type: research

EZH2 promotes cell proliferation by regulating the expression of RUNX3 in laryngeal carcinoma.
Abstract Enhancer of zeste homolog 2 (EZH2) is a highly conserved histone methyltransferase, which is overexpressed in different types of cancers such as breast and prostate cancer. It is reported that EZH2 can directly down-regulate RUNX3 by increasing histone H3 methylation. However, the role of EZH2 in the development and progression of laryngeal carcinoma has not yet been investigated, and the relationship between EZH2 and RUNX3 in laryngeal carcinoma is rarely reported. The current study aims to determine the role of EZH2 in the progression of laryngeal carcinoma, and investigate the interaction between EZH2 ...
Source: Molecular and Cellular Biochemistry - August 9, 2017 Category: Biochemistry Authors: Lian R, Ma H, Wu Z, Zhang G, Jiao L, Miao W, Jin Q, Li R, Chen P, Shi H, Yu W Tags: Mol Cell Biochem Source Type: research

Role of curcumin in PLD activation by Arf6-cytohesin1 signaling axis in U46619-stimulated pulmonary artery smooth muscle cells.
Abstract Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine to produce phosphatidic acid (PA) which in some cell types play a pivotal role in agonist-induced increase in NADPH oxidase-derived [Formula: see text]production. Involvement of ADP ribosylation factor (Arf) in agonist-induced activation of PLD is known for smooth muscle cells of systemic arteries, but not in pulmonary artery smooth muscle cells (PASMCs). Additionally, role of cytohesin in this scenario is unknown in PASMCs. We, therefore, determined the involvement of Arf and cytohesin in U46619-induced stimulation of PLD in PASMCs, an...
Source: Molecular and Cellular Biochemistry - August 5, 2017 Category: Biochemistry Authors: Chakraborti S, Sarkar J, Bhuyan R, Chakraborti T Tags: Mol Cell Biochem Source Type: research

Inhibition of protein kinase CK2 sensitizes non-small cell lung cancer cells to cisplatin via upregulation of PML.
In conclusion, inhibiting CK2 can enhance sensitivity of CDDP to NSCLC cancer cells through PML. PMID: 28744813 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - July 25, 2017 Category: Biochemistry Authors: Yang B, Yao J, Li B, Shao G, Cui Y Tags: Mol Cell Biochem Source Type: research

MiRNA-200a induce cell apoptosis in renal cell carcinoma by directly targeting SIRT1.
Abstract Accumulating evidence indicates that microRNAs are implicated in tumor initiation and progression through negatively regulating oncogenes or tumor suppressor genes. In the present study, we report that the expression of miR-200a was significantly lower in renal cell carcinoma (RCC) specimens and RCC cell lines. Restoration of miR-200a suppressed cell growth, arrested cell cycle progression, and promoted cell apoptosis in RCC cell lines. We next used qRT-PCR array technology to identify Sirtuin 1 (SIRT1) as one of the downregulated proteins during miR-200a overexpression in 786-O cells. Following a further...
Source: Molecular and Cellular Biochemistry - July 17, 2017 Category: Biochemistry Authors: Fu H, Song W, Chen X, Guo T, Duan B, Wang X, Tang Y, Huang L, Zhang C Tags: Mol Cell Biochem Source Type: research

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