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Nrf2/ARE pathway activation is involved in negatively regulating heat-induced apoptosis in non-small cell lung cancer cells.
In this study, using immunocytochemistry and western blot analysis, we demonstrated that heat stress induced Nrf2/ARE activation through the nuclear translocation of Nrf2 in non-small cell lung cancer cells. Luciferase activity was also increased. Additionally, antioxidant enzymes were increased through Nrf2 activation after heat stress. Transfection of lung cancer cells with siRNA directed against Nrf2 increased heat cytotoxicity and cell apoptosis. Heat stress could induce reactive oxygen species (ROS) accumulation, while the antioxidant NAC obviously reduced cell apoptosis ratio, indicating that heat stress induced cell...
Source: Acta Biochimica et Biophysica Sinica - April 6, 2020 Category: Biochemistry Authors: Xie W, Tan B, Yang Z, Yu X, Chen L, Ran D, Xu Q, Zhou X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

FoxM1 affects adhesive, migratory, and invasive abilities of human retinoblastoma Y-79 cells by targeting matrix metalloproteinase 2.
In this study, we aimed to investigate the correlation between FoxM1 and retinoblastoma (Rb) metastasis and to explore the detailed mechanism. Wound healing, cell adhesion, and invasion assays showed that FoxM1 overexpression induced epithelial-mesenchymal transition in Y-79 cells and inhibited adhesion and subsequently promoted metastasis of Y-79 cells, while FoxM1 knockdown showed the opposite effects. A luciferase reporter assay and chromatin immunoprecipitation assay provided evidence that FoxM1 promoted matrix metalloproteinase 2 (MMP2) transcription by directly binding to and promoting MMP2 promoter. MMP2 knockdown b...
Source: Acta Biochimica et Biophysica Sinica - March 9, 2020 Category: Biochemistry Authors: Zhu X, Yu M, Wang K, Zou W, Zhu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Resveratrol attenuates diabetes-associated cell centrosome amplification via inhibiting the PKC α-p38 to c-myc/c-jun pathway.
In conclusion, our results suggest that PKCα-p38 to c-myc/c-jun is the signaling pathway of the diabetes-associated centrosome amplification, and resveratrol attenuates the centrosome amplification by inhibiting this signaling pathway. PMID: 31844893 [PubMed - as supplied by publisher]
Source: Acta Biochimica et Biophysica Sinica - December 15, 2019 Category: Biochemistry Authors: Wu Q, Chen X, He Q, Lang L, Xu P, Wang P, Lee SC Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

YAP mediates the positive regulation of hnRNPK on the lung adenocarcinoma H1299 cell growth.
In this study, we observed that the expression pattern of hnRNPK in H1299 lung adenocarcinoma cells varied depending on the cell density in culture. Moreover, hnRNPK stimulated the ability of proliferation and colony formation of H1299 cells, which is important for the multilayered cell growth in culture. We further investigated whether there is an association between hnRNPK and the elements involved in the cell contact inhibition pathway. By using quantitative reverse transcriptase-polymerase chain reaction assay and a YAP activity reporter system, we found that hnRNPK upregulated the mRNA and protein levels and transcrip...
Source: Acta Biochimica et Biophysica Sinica - June 11, 2019 Category: Biochemistry Authors: Xu L, Zhang T, Huang W, Liu X, Lu J, Gao X, Zhang YF, Liu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Downregulation of long non-coding RNA MEG3 promotes proliferation, migration, and invasion of human hepatocellular carcinoma cells by upregulating TGF- β1.
In this study, we aimed to investigate the role of lncRNA maternally expressed gene 3 (MEG3) in the development and progression of hepatocellular carcinoma. Expression of MEG3 in tumor tissues and adjacent healthy tissues of hepatocellular carcinoma patients, as well as the serum of both hepatocellular carcinoma patients and healthy controls, was detected by quantitative reverse transcriptase-polymerase chain reaction. The results showed that expression level of MEG3 was significantly lower in tumor tissues than in adjacent healthy tissues. Serum level of MEG3 was also significantly lower in hepatocellular carcinoma patien...
Source: Acta Biochimica et Biophysica Sinica - May 13, 2019 Category: Biochemistry Authors: Dong H, Zhang Y, Xu Y, Ma R, Liu L, Luo C, Jiang W Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Prox1 is essential for oligodendrocyte survival and regulates oligodendrocyte apoptosis via the regulation of NOXA.
Abstract Demyelinating diseases, such as multiple sclerosis, are known to result from acute or chronic injury to the myelin sheath and inadequate remyelination. Its underlying molecular mechanisms, however, remain unclear. The transcription factor prospero homeobox 1 (Prox1) plays an essential role during embryonic development of the central nervous system and cell differentiation. Thus, we aimed to investigate the role of Prox1 in the survival and differentiation of oligodendrocytes. Cell viability was measured by MTT assay. Flow cytometry was conducted to analyze cell apoptosis. Ectopic-Prox1 and shProx1 were us...
Source: Acta Biochimica et Biophysica Sinica - June 20, 2018 Category: Biochemistry Authors: Chang W, Teng J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Integrin β3 inhibits hypoxia-induced apoptosis in cardiomyocytes.
Abstract Hypoxia-induced apoptosis plays an important role in cardiovascular diseases. Integrin β3 is one of the main integrin heterodimer receptors on the surface of cardiac myocytes. However, despite the important role that integrin β3 plays in the cardiovascular disease, its exact role in the hypoxia response remains unclear. Hence, in the present investigation we aimed to study the role of integrin β3 in hypoxia-induced apoptosis in H9C2 cells and primary rat myocardial cells. MTT assay, flow cytometry and TUNEL assay results showed that hypoxia inhibited cardiomyocyte proliferation and induced cardiomyocyt...
Source: Acta Biochimica et Biophysica Sinica - May 23, 2018 Category: Biochemistry Authors: Su Y, Tian H, Wei L, Fu G, Sun T Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Mixed lineage kinase domain-like protein induces RGC-5 necroptosis following elevated hydrostatic pressure.
Abstract Receptor-interacting protein 3 (RIP3) is an essential component of the necroptosis signaling pathway. Phosphorylation of its downstream target, mixed lineage kinase domain-like protein (MLKL), has been proposed to induce necroptosis by initiating Ca2+ influx. Our previous studies have shown that RGC-5 retinal ganglion cells undergo RIP3-mediated necroptosis following elevated hydrostatic pressure (EHP). However, the molecular mechanism underlying necroptosis induction downstream of RIP3 is still not well understood. Here, we investigated the effects of MLKL during EHP-induced necroptosis, and primarily ex...
Source: Acta Biochimica et Biophysica Sinica - October 1, 2017 Category: Biochemistry Authors: Liao L, Shang L, Li N, Wang S, Wang M, Huang Y, Chen D, Huang J, Xiong K Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

The histone acetylation mediated by Gcn5 regulates the Hoxc11 gene expression in MEFs.
In this study, we propose to investigate the role of Gcn5, a histone acetyltransferase, in the regulation of Hoxc11 expression in MEFs. We showed that the H3 lysine 9 acetylation (H3K9ac) status has the same correlation with Hoxc11 expression and reported that Gcn5 is associated with the upregulation of Hoxc11 expression through H3K9ac in Akt1-/- MEFs. Since Hoxc11 was upregulated through histone acetylation in Akt1-/- MEFs, a functional role of Gcn5 on Hoxc11 expression was analyzed in Akt1-/- MEFs treated with Gcn5 specific inhibitor or transfected with Gcn5-small interfering RNA (Gcn5-siRNA). When the expression of Hoxc...
Source: Acta Biochimica et Biophysica Sinica - May 24, 2017 Category: Biochemistry Authors: Oh JH, Lee JY, Kong KA, Kim JM, Kim MH Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research