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Transcription factor AP-2α regulates acute myeloid leukemia cell proliferation by influencing Hoxa gene expression.
Abstract Transcription factor AP-2α mediates transcription of a number of genes implicated in mammalian development, cell proliferation and carcinogenesis. In the current study, we identified Hoxa7, Hoxa9 and Hox cofactor Meis1 as AP-2α target genes, which are involved in myeloid leukemogenesis. Luciferase reporter assays revealed that overexpression of AP-2α activated transcription activities of Hoxa7, Hoxa9 and Meis1, whereas siRNA of AP-2α inhibited their transcription activities. We found that AP-2 binding sites in regulatory regions of three genes activated their transcription by mutant analysis and AP-2Î...
Source: The International Journal of Biochemistry and Cell Biology - May 6, 2013 Category: Biochemistry Authors: Ding X, Yang Z, Zhou F, Wang F, Li X, Chen C, Li X, Hu X, Xiang S, Zhang J Tags: Int J Biochem Cell Biol Source Type: research

The self-renewal of mouse embryonic stem cells is regulated by cell-substratum adhesion and cell spreading.
Abstract Mouse embryonic stem cells (mESCs) undergo self-renewal in the presence of the cytokine, leukaemia inhibitory factor (LIF). Following LIF withdrawal, mESCs differentiate, and this is accompanied by an increase in cell-substratum adhesion and cell spreading. The purpose of this study was to investigate the relationship between cell spreading and mESC differentiation. Using E14 and R1 mESC lines, we have restricted cell spreading in the absence of LIF by either culturing mESCs on chemically defined, weakly adhesive biomaterial substrates, or by manipulating the cytoskeleton. We demonstrate that by restricti...
Source: The International Journal of Biochemistry and Cell Biology - July 17, 2013 Category: Biochemistry Authors: Murray P, Prewitz M, Hopp I, Wells N, Zhang H, Cooper A, Parry KL, Short R, Antoine DJ, Edgar D Tags: Int J Biochem Cell Biol Source Type: research