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Ultrasound-mediated vascular endothelial growth factor C (VEGF-C) gene microbubble transfection inhibits growth of MCF-7 breast cancer cells.
Abstract We evaluated the effects of ultrasound-mediated microbubble transfection of VEGF-C siRNA on breast cancer cells in vitro and in vivo. MCF-7 cells were transfected with VEGF-C siRNA and the protein and mRNA expression of VEGF-C was tested using Western blot and qRT-PCR. Twenty nude mice tumors were established by injecting with MCF-7 cells, and were randomized into four groups when palpable tumors reached 190 mm3. The length and width of MCF-7 tumors in mice were measured every 3 days. After 20 days, all mice were killed and the expression of VEGF-C in tumor tissue was also detected by Western blot and qRT...
Source: Oncology Research - July 28, 2013 Category: Cancer & Oncology Authors: Xu Q, Sun T, Tian H, Wang C, Zhou H Tags: Oncol Res Source Type: research

Hypoxia-induced autophagy confers resistance of breast cancer cells to ionizing radiation.
Authors: He WS, Dai XF, Jin M, Liu CW, Rent JH Abstract Hypoxia is a hallmark of solid tumors, which presents a major obstacle to the effectiveness of radiation therapy. However, the function and the importance of molecular response have not been well defined. In the present study, hypoxia-induced autophagy and its effect on the response of breast cancer cells to ionizing radiation were examined. Results showed that hypoxic exposure induced a marked accumulation of autophagosomes accompanied by mRNA induction of the autophagy-related genes Beclin-1, Atg5, Atg7, and Atg12. The elevated autophagic activity was associ...
Source: Oncology Research - December 1, 2014 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Inhibition of Lung Carcinoma A549 Cell Growth by Knockdown of Hexokinase 2 In Situ and In Vivo.
In this study, we explored the functional role of HK2 in lung cancer cell proliferation and tumorigenesis and determine its expression profile in lung cancer. HK2 expression was increased in primary lung cancer tissues of patients. Knocking down HK2 expression by small interfering RNA (siRNA) inhibited cell proliferation in lung cancer cells and nude mice. Thus, HK2 is required for sustained proliferation and survival of tumor cells in vitro and in vivo, and its aberrant expression may contribute to the pathogenesis of lung cancer. Thus, our study provided evidence that HK2 functions as a novel oncogene in lung cancer and ...
Source: Oncology Research - January 24, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

MicroRNA-664 targets Insulin receptor substrate 1 to suppress cell proliferation and invasion in breast cancer.
This study showed that miR-664 expression was obviously reduced in BC tissues and cell lines. Resumption expression of miR-664 attenuated the proliferation and invasion of BC cells. The molecular mechanism underlying the inhibitory effects of BC cell proliferation and invasion by miR-664 was also studied. Insulin receptor substrate 1 (IRS1) was identified as a novel and direct target of miR-664. In addition, siRNA-mediated silencing of IRS1 expression mimicked the suppressive effects of miR-664 overexpression in BC cells. Rescue experiments demonstrated that recovered IRS1 expression partially antagonized the inhibition of...
Source: Oncology Research - March 3, 2018 Category: Cancer & Oncology Tags: Oncol Res Source Type: research