Using MetaboAnalyst 5.0 for LC–HRMS spectra processing, multi-omics integration and covariate adjustment of global metabolomics data
Nature Protocols, Published online: 17 June 2022; doi:10.1038/s41596-022-00710-wLC–HRMS is used for metabolomics studies in the biomedical and environmental sciences. MetaboAnalyst (metaboanalyst.ca) can be used to address challenges in data processing, statistical analysis, functional interpretation and multi-omics integration. (Source: Nature Protocols)
Source: Nature Protocols - June 17, 2022 Category: Biomedical Science Authors: Zhiqiang Pang Guangyan Zhou Jessica Ewald Le Chang Orcun Hacariz Niladri Basu Jianguo Xia Source Type: research
Multigenerational laboratory culture of pelagic ctenophores and CRISPR–Cas9 genome editing in the lobate Mnemiopsis leidyi
Nature Protocols, Published online: 13 June 2022; doi:10.1038/s41596-022-00702-wThis protocol enables users to establish long-term multigenerational ctenophore cultures in the laboratory and details CRISPR–Cas9 genome editing in Mnemiopsis leidyi. (Source: Nature Protocols)
Source: Nature Protocols - June 13, 2022 Category: Biomedical Science Authors: J. S. Presnell M. Bubel T. Knowles W. Patry W. E. Browne Source Type: research
Molecular profiling of enteric nervous system cell lineages
Nature Protocols, Published online: 08 June 2022; doi:10.1038/s41596-022-00697-4Fluorescently labeled nuclei of the enteric nervous system are isolated from adult mouse or zebrafish gut and characterized by nuclear RNA sequencing and visualization and quantification of transcripts using a custom analytical pipeline. (Source: Nature Protocols)
Source: Nature Protocols - June 8, 2022 Category: Biomedical Science Authors: Yuuki Obata Álvaro Castaño Todd L. Fallesen Ana Carina Bon-Frauches Stefan Boeing Almaz Huseynova Sarah McCallum Reena Lasrado Tiffany A. Heanue Vassilis Pachnis Source Type: research
Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView
Nature Protocols, Published online: 06 June 2022; doi:10.1038/s41596-022-00688-5Sulc and colleagues describe a design tool, oxView, for visualization and analysis of simulations of DNA, RNA and nucleic acid–protein nanostructures. (Source: Nature Protocols)
Source: Nature Protocols - June 6, 2022 Category: Biomedical Science Authors: Joakim Bohlin Michael Matthies Erik Poppleton Jonah Procyk Aatmik Mallya Hao Yan Petr Šulc Source Type: research
The normative modeling framework for computational psychiatry
Nature Protocols, Published online: 01 June 2022; doi:10.1038/s41596-022-00696-5This protocol guides the user through normative modeling analysis using the Predictive Clinical Neuroscience toolkit (PCNtoolkit), enabling individual differences to be mapped at the level of a single subject or observation in relation to a reference model. (Source: Nature Protocols)
Source: Nature Protocols - June 1, 2022 Category: Biomedical Science Authors: Saige Rutherford Seyed Mostafa Kia Thomas Wolfers Charlotte Fraza Mariam Zabihi Richard Dinga Pierre Berthet Amanda Worker Serena Verdi Henricus G. Ruhe Christian F. Beckmann Andre F. Marquand Source Type: research
