Prevalence of < i > Campylobacter < /i > spp. in diarrhoea samples from patients in New South Wales, Australia
In this study, 400 human clinical fecal samples were collected from two NSW locations, Western Sydney and Wagga Wagga, and tested for the presence of Campylobacter spp. Patients were clustered by location, age and gender to assess Campylobacter spp. prevalence within these groups between the two regions. The frequency of Campylobacter spp. was higher in males compared to females in the age groups 0–4 and 5–14 years; 6.4% and 1.0%, and 8.2% and none, respectively (P< 0.05). A second peak was noted in elderly adults compared with those in younger age groups. Based on the findings of the quantitative PCR analys...
Source: International Microbiology - October 17, 2016 Category: Microbiology Source Type: research
Genetic and serologic surveillance of rotavirus with P[8] and P[4] genotypes in feces from children in the city of Chihuahua, northern Mexico
This study investigated the G and P rotavirus genotypes circulating in children from two hospitals in the city of Chihuahua, Mexico. Additionally, polyclonal antibodies against Rotavirus Wa strain were used to determine their homotypic and heterotypic reactivity to both P[8] and P[4] genotypes. G1, G2, and G3 VP7 genotypes and P[8] and P[4] VP4 genotypes were detected in common and uncommon combinations as well as mixed infectious. The predominant combination was G1P[8]. Phylogenetic analysis of VP4 gene revealed the presence of P[8]-1 and P[8]-3 lineages of P[8] genotype and P[4]-5 lineage of P[4] genotype. All but five G...
Source: International Microbiology - October 17, 2016 Category: Microbiology Source Type: research
Haloalkalitolerant Actinobacteria with capacity for anthracene degradation isolated from soils close to areas with oil activity in the State of Veracruz, Mexico
In this study, samples of soils close to areas with oil activity in the State of Veracruz, Mexico, were inoculated for the isolation of Actinobacteria. The strains isolated were characterized morphologically, and the concentrations of NaCl and pH were determined for optimal growth. Strain selection was performed by the detection of a phylogenetic marker for Actinobacteria located at the 23S rRNA gene, followed by species identification by sequencing the 16S rRNA gene. Several haloalkalitolerant Actinobacteria were isolated and identified as: Kocuria rosea, K. palustris, Microbacterium testaceum, Nocardia farcinica and Cell...
Source: International Microbiology - October 17, 2016 Category: Microbiology Source Type: research
Annual indexes
(Source: International Microbiology)
Source: International Microbiology - September 6, 2016 Category: Microbiology Source Type: research
Chromosomes of Protists: The crucible of evolution
As early as 1925, the great protozoologist Edouard Chatton classified microorganisms into two categories, the prokaryotic and the eukaryotic microbes, based on light microscopical observation of their nuclear organization. Now, by means of transmission electron microscopy, we know that prokaryotic microbes are characterized by the absence of nuclear envelope surrounding the bacterial chromosome, which is more or less condensed and whose chromatin is deprived of histone proteins but presents specific basic proteins. Eukaryotic microbes, the protists, have nuclei surrounded by a nuclear envelope and have chromosomes more or ...
Source: International Microbiology - September 6, 2016 Category: Microbiology Source Type: research
Archaeal and bacterial diversity in two hot springs from geothermal regions in Bulgaria as demostrated by 16S rRNA and GH-57 genes
Archaeal and bacterial diversity in two Bulgarian hot springs, geographically separated with different tectonic origin and different temperature of water was investigated exploring two genes, 16S rRNA and GH-57. Archaeal diversity was significantly higher in the hotter spring Levunovo (LV) (82°C); on the contrary, bacterial diversity was higher in the spring Vetren Dol (VD) (68°C). The analyzed clones from LV library were referred to twenty eight different sequence types belonging to five archaeal groups from Crenarchaeota and Euryarchaeota. A domination of two groups was observed, Candidate Thaumarchaeota and Meth...
Source: International Microbiology - May 26, 2016 Category: Microbiology Source Type: research
Zoonoses. Infectious diseases transmissible from animals to humans. Bauerfeind R, von Graevenitz A, Kimmig P, Schiefer HG, Schwarz T, Slenczka W, Zahner H (eds)
(Source: International Microbiology)
Source: International Microbiology - May 26, 2016 Category: Microbiology Source Type: research
Hypercompetition in biomedical research evaluation and its impact on young scientist careers
Recent years have seen tremendous changes in the modes of publication and dissemination of biomedical information, with the introduction of countless new publishers and publishing models, as well as alternative modes of research evaluation. In parallel, we are witnessing an unsustainable explosion in the amount of information generated by each individual scientist, at the same time as many countries’ shrinking research budgets are greatly increasing the competition for research funding. In such a hypercompetitive environment, how does one measure excellence? This contribution will provide an overview of some of the o...
Source: International Microbiology - May 26, 2016 Category: Microbiology Source Type: research
Interaction and cooperative effort among scientific societies. Twelve years of COSCE
The evolution of knowledge and technology in recent decades has brought profound changes in science policy, not only in the countries but also in the supranational organizations. It has been necessary, therefore, to adapt the scientific institutions to new models in order to achieve a greater and better communication between them and the political counterparts responsible for defining the general framework of relations between science and society. The Federationon of Scientific Societies of Spain (COSCE, Confederación de Sociedades Científicas de España) was founded in October 2003 to respond to the ur...
Source: International Microbiology - May 26, 2016 Category: Microbiology Source Type: research
A < i > Kluyveromyces marxianus < /i > 2-deoxyglucose-resistant mutant with enhanced activity of xylose utilization
< p > Thermotolerant ethanologenic yeast Kluyveromyces marxianus is capable of fermenting various sugars including xylose but glucose represses to hamper the utilization of other sugars. To acquire glucose repression-defective strains, 33 isolates as 2-deoxyglucose (2-DOG)-resistant mutants were acquired from about 100 colonies grown on plates containing 2-DOG, which were derived from an efficient strain DMKU 3-1042. According to the characteristics of sugar consumption abilities and cell growth and ethanol accumulation along with cultivation time, they were classified into three groups. The first group (3 isolates) utiliz...
Source: International Microbiology - May 26, 2016 Category: Microbiology Source Type: research
A Kluyveromyces marxianus 2-deoxyglucose-resistant mutant with enhanced activity of xylose utilization
Thermotolerant ethanologenic yeast Kluyveromyces marxianus is capable of fermenting various sugars including xylose but glucose represses to hamper the utilization of other sugars. To acquire glucose repression-defective strains, 33 isolates as 2-deoxyglucose (2-DOG)-resistant mutants were acquired from about 100 colonies grown on plates containing 2-DOG, which were derived from an efficient strain DMKU 3-1042. According to the characteristics of sugar consumption abilities and cell growth and ethanol accumulation along with cultivation time, they were classified into three groups. The first group (3 isolates) utilized glu...
Source: International Microbiology - May 26, 2016 Category: Microbiology Source Type: research
< i > Chlamydia pneumoniae < /i > CPj0783 interaction with Huntingtin-protein14
< p > Chlamydia pneumoniae is a Gram-negative, obligate intracellular pathogen that causes community-acquired respiratory infections. After C. pneumoniae invades host cells, it disturbs the vesicle transport system to escape host lysosomal or autophagosomal degradation. By using a yeast mis-sorting assay, we found 10 C. pneumoniae candidate genes involved in aberrant vesicular trafficking in host cells. One of the candidate genes, CPj0783, was recognized by antibodies from C. pneumoniae-infected patients. The expression of CPj0783 was detected at mid to late-cycle time points and increased during the inclusion maturation. ...
Source: International Microbiology - May 26, 2016 Category: Microbiology Source Type: research
Chlamydia pneumoniae CPj0783 interaction with Huntingtin-protein14
Chlamydia pneumoniae is a Gram-negative, obligate intracellular pathogen that causes community-acquired respiratory infections. After C. pneumoniae invades host cells, it disturbs the vesicle transport system to escape host lysosomal or autophagosomal degradation. By using a yeast mis-sorting assay, we found 10 C. pneumoniae candidate genes involved in aberrant vesicular trafficking in host cells. One of the candidate genes, CPj0783, was recognized by antibodies from C. pneumoniae-infected patients. The expression of CPj0783 was detected at mid to late-cycle time points and increased during the inclusion maturation. Two-hy...
Source: International Microbiology - May 26, 2016 Category: Microbiology Source Type: research
Year ’s comments for 2015
(Source: International Microbiology)
Source: International Microbiology - May 25, 2016 Category: Microbiology Source Type: research
The 25th SEM Congress (Logroño, Spain, July 7–10, 2015)
The 25th Congress of the Spanish Society for Microbiology (SEM) took place on 7–10 July, 2015, at the University of La Rioja, in Logroño. This meeting brought together microbiologists from several prestigious universities and research centers throughout Spain, as well as experts from other countries including the United States, United Kingdom, Portugal, Germany, Mexico and Venezuela. The program included an opening lecture, one invited lecture and a closing lecture, twelve symposia on selected topics, ten sessions of oral presentations, four poster sessions, and three workshops. There were around 230 poster pr...
Source: International Microbiology - April 7, 2016 Category: Microbiology Source Type: research
