Comparison of solid-phase red cell adherence and microcolumn agglutination technology using untreated and enzyme-treated red blood cells
This study was undertaken to detect and identify alloantibodies by performing antibody screen (ABS) and antibody identification (ABID) testing using SPRCA and CAT, with and without ficin-treated RBCs. Residual patient samples collected between 1 December 2020 and 19 May 2021 were saved, de-identified, and frozen at ≤-30°C before testing for alloantibodies. Seventy antibodies were detected in 53 samples among the 203 samples that underwent an ABS. Of those samples, 150 (73.0%) were nonreactive, 47 (23.1%) yielded positive results with both CAT and SPRCA, and six (3.0%) yielded positive ABS results with SPRCA only. Fifty-...
Source: Immunohematology - January 5, 2024 Category: Hematology Authors: D R Sanders Source Type: research
Peter D. Issitt (1933-2023)
Immunohematology. 2023 Dec 29;39(4):181. doi: 10.2478/immunohematology-2023-026. eCollection 2023 Dec 1.NO ABSTRACTPMID:38179783 | DOI:10.2478/immunohematology-2023-026 (Source: Immunohematology)
Source: Immunohematology - January 5, 2024 Category: Hematology Authors: Patricia Tippett Source Type: research
To contributors to the 2023 issues
Immunohematology. 2023 Dec 29;39(4):182-183. doi: 10.2478/immunohematology-2023-027. eCollection 2023 Dec 1.NO ABSTRACTPMID:38179784 | DOI:10.2478/immunohematology-2023-027 (Source: Immunohematology)
Source: Immunohematology - January 5, 2024 Category: Hematology Source Type: research
Evaluation of solid-phase panreactivity with negative direct antiglobulin testing
Immunohematology. 2023 Dec 29;39(4):151-154. doi: 10.2478/immunohematology-2023-022. eCollection 2023 Dec 1.ABSTRACTSolid-phase red cell adherence (SPRCA) is a sensitive platform for antibody detection, but nonspecific reactions may occur. One pattern of apparent nonspecific reactivity is a panagglutinin with a negative direct antiglobulin test (DAT). The purpose of this study was to define the clinical characteristics of patients with these nonspecific reactions and their associated serologic findings. Twenty patients with panreactive SPRCA testing results were identified between November 2022 and May 2023. In addition to...
Source: Immunohematology - January 5, 2024 Category: Hematology Authors: J T Little D P Blackall Source Type: research
Feasibility and performance of in-house red blood cell reagents to detect unexpected antibodies in immunized patients in Burkina Faso
This study aimed at investigating the feasibility and performance of locally prepared RBC reagents for antibody detection in Burkina Faso. We conducted an experimental study comparing commercial RBC panels and a local panel prepared from phenotyped blood donors in Ouagadougou, Burkina Faso. Antibody detection testing was performed by the indirect antiglobulin test using a gel card filtration column in a low-ionic-strength solution. Judgment criteria were the concordance rate and the kappa agreement coefficient of results generated by the two panels. A total of 302 blood donors were phenotyped for the major antigens of the ...
Source: Immunohematology - January 5, 2024 Category: Hematology Authors: S Sawadogo K Nebie D Kima S K A Ouedraogo P A Nagnon J Koulidiati C Traore S Sawadogo E Kafando V Deneys Source Type: research
