Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Comparison of estimations of urinary bladder volume in different scanning positions using 2D linear dimension formula and 3D bladder circumference tracing in client-owned cats
Can J Vet Res. 2024 Apr;88(2):55-65.ABSTRACTUrinary bladder volume (UBV) can be estimated using point-of-care ultrasound. The purpose of this study was to compare 2 UBV estimation methods, i.e., three-dimensional (3D) bladder circumference tracing and 2-dimensional (2D) linear bladder dimension formula, against actual bladder volumes in awake client-owned cats and identify the best scanning position for UBV estimations. Up to 3 paired sets of orthogonal longitudinal and transverse bladder ultrasound images were acquired by a trained clinician from 21 cats positioned in dorsal, right lateral, and left lateral recumbency. UB...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: Sabrina Ayoub Xiu Ting Yiew Gabrielle Monteith Allan R Willms Source Type: research

Prevalence of pathogens in honey bee colonies and association with clinical signs in southwestern Quebec, Canada
Can J Vet Res. 2024 Apr;88(2):45-54.ABSTRACTHoney bees can be affected by a variety of pathogens, which impacts their vital role as pollinators in agriculture. A cross-sectional study was conducted in southwestern Quebec to: i) estimate the prevalence of 11 bee pathogens; ii) assess the agreement between beekeeper suspicion of a disease and laboratory detection of the causative pathogen; and iii) explore the association between observed clinical signs and pathogen detection in a colony. A total of 242 colonies in 31 apiaries owned by 15 beekeepers was sampled in August 2017. The prevalence of Varroa destructor detection wa...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: Gabrielle Claing Pascal Dubreuil Martine Bernier Julie Ferland Yvan L'Homme Edisleidy Rodriguez Julie Arsenault Source Type: research

Comparison of blood profiles between housed and grazing Korean indigenous cattle (Hanwoo)
The objective of this study was to compare the hematology profiles of Korean indigenous cattle (Hanwoo) raised in a barn (housed) or on pasture (grazing). Our findings showed significant differences in the red blood cell (RBC) profiles of these 2 groups. When compared to cattle raised in a barn, a significant decrease in hematocrit (P = 0.000), hemoglobin (P = 0.000), and red blood cells (RBCs) (P = 0.000) and a significant increase in mean cell volume (P = 0.015) and reticulocytes (P = 0.000) were observed in grazing cattle, which indicate regenerative anemia. Furthermore, indirect bilirubin was significantly higher in gr...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: Youngjun Kim Ji-Yeong Ku Kwang-Man Park Jonghun Baek Kyoung-Seong Choi Jinho Park Source Type: research

Production of a new tetravalent vaccine targeting fimbriae and enterotoxin of enterotoxigenic < em > Escherichia coli < /em >
The objective of this study was to prepare a novel tetravalent vaccine to effectively prevent piglet diarrhea caused by E. coli. In order to realize the production of K88ac-K99-ST1-LTB tetravalent inactivated vaccine, the biological characteristics, stability, preservation conditions, and safety of the recombinant strain BL21(DE3) (pXKKSL4) were studied, and the vaccine efficacy and minimum immune dose were measured. The results indicated that the biological characteristics, target protein expression, and immunogenicity of the 1st to 10th generations of the strain were stable. Therefore, the basic seed generation was preli...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: ChongLi Xu Yuhan She Fengyang Fu ChongBo Xu Source Type: research

Comparison of estimations of urinary bladder volume in different scanning positions using 2D linear dimension formula and 3D bladder circumference tracing in client-owned cats
Can J Vet Res. 2024 Apr;88(2):55-65.ABSTRACTUrinary bladder volume (UBV) can be estimated using point-of-care ultrasound. The purpose of this study was to compare 2 UBV estimation methods, i.e., three-dimensional (3D) bladder circumference tracing and 2-dimensional (2D) linear bladder dimension formula, against actual bladder volumes in awake client-owned cats and identify the best scanning position for UBV estimations. Up to 3 paired sets of orthogonal longitudinal and transverse bladder ultrasound images were acquired by a trained clinician from 21 cats positioned in dorsal, right lateral, and left lateral recumbency. UB...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: Sabrina Ayoub Xiu Ting Yiew Gabrielle Monteith Allan R Willms Source Type: research

Prevalence of pathogens in honey bee colonies and association with clinical signs in southwestern Quebec, Canada
Can J Vet Res. 2024 Apr;88(2):45-54.ABSTRACTHoney bees can be affected by a variety of pathogens, which impacts their vital role as pollinators in agriculture. A cross-sectional study was conducted in southwestern Quebec to: i) estimate the prevalence of 11 bee pathogens; ii) assess the agreement between beekeeper suspicion of a disease and laboratory detection of the causative pathogen; and iii) explore the association between observed clinical signs and pathogen detection in a colony. A total of 242 colonies in 31 apiaries owned by 15 beekeepers was sampled in August 2017. The prevalence of Varroa destructor detection wa...
Source: Canadian Journal of Veterinary Research - April 10, 2024 Category: Veterinary Research Authors: Gabrielle Claing Pascal Dubreuil Martine Bernier Julie Ferland Yvan L'Homme Edisleidy Rodriguez Julie Arsenault Source Type: research

Ability of unsterilized recycled manure solids bedding to support growth of < em > Klebsiella pneumoniae < /em > and < em > Escherichia coli < /em >
The objective of this study was to describe bacterial growth in three different types of RMS bedding, as well as in sand, when samples were experimentally inoculated with Escherichia coli and Klebsiella pneumoniae. Two 3-day trials were conducted, during which treatments included inoculating bedding samples with E. coli and K. pneumoniae, as well as no inoculation. The trial was repeated 3 times for each bedding sample on each day. Samples were incubated at 15°C for 3 d and bacterial counts were measured every day. After inoculation, there was no significant K. pneumoniae or E. coli growth phase during the trial in those ...
Source: Canadian Journal of Veterinary Research - January 15, 2024 Category: Veterinary Research Authors: Annie Fr échette Myl ène Généreux Gilles Fecteau Caroline C ôté Simon Dufour Source Type: research

Retrospective study of perioperative antimicrobial use in horses undergoing elective laparoscopy at a single institution
The objective of this study was to evaluate practices associated with perioperative antimicrobial use in equine elective laparoscopy at a single institution over a 21-year period and to determine whether antimicrobial therapy influenced the occurrence of postoperative complications. Medical records of horses that underwent elective laparoscopy at a teaching hospital from January 2000 to September 2021 were reviewed. Data obtained included signalment, surgeon, type and duration of procedure, perioperative antimicrobial use, and intraoperative and postoperative complications. Exact univariate logistic regression was used to ...
Source: Canadian Journal of Veterinary Research - January 15, 2024 Category: Veterinary Research Authors: Lucciana Recchi Nicola Cribb Nathalie C ôté Marie-Soleil Dubois Judith Koenig Alexander Valverde Gabrielle Monteith Source Type: research

Development and validation of a farm- and province-level swine flow simulation model using discrete events and Ontario swine farm and provincial input data
Can J Vet Res. 2024 Jan;88(1):3-11.ABSTRACTInfectious disease events can cause disruptions in service-based and agricultural industries. The list of possible events is long and varies from the incursion or emergence of a reportable animal pathogen to the recently documented interruptions caused by the COVID-19 pandemic. There is a need to develop models that can determine the impact of pathogens and mitigation measures on populations that are not directly affected by the pathogen in the case of a reportable disease, particularly when the health and welfare of these populations could be affected due to resulting disruptions...
Source: Canadian Journal of Veterinary Research - January 15, 2024 Category: Veterinary Research Authors: Maggie Henry Wade McDonald Robert M Friendship Amy L Greer Zvonimir Poljak Source Type: research

Metabolic response of broiler chickens to different doses of < em > Enterococcus faecium < /em > ICIS 96 in the diet
The objectives of this study were to identify the effect of various doses of Enterococcus faecium ICIS 96 added to the diet of broiler chickens on their metabolism and to evaluate the productive qualities of the chickens when different doses are used. The experiment was carried out on 72 Cobb-500 cross broiler chickens, divided into 3 groups. Chickens in the first group received a suspension of E. faecium ICIS 96 containing 1 × 108 cells in 1 mL of sterile saline, at a dose of 0.1 mL per 1 kg of live weight. The second group of chickens received a double dose of enterococcus, that is 0.2 mL of the suspension per 1 kg of l...
Source: Canadian Journal of Veterinary Research - January 15, 2024 Category: Veterinary Research Authors: Elena Kochkina Alfia Andreeva Alexey Torshkov Veronika Dymova Oleg Altynbekov Mariia Sycheva Source Type: research

Ability of unsterilized recycled manure solids bedding to support growth of < em > Klebsiella pneumoniae < /em > and < em > Escherichia coli < /em >
The objective of this study was to describe bacterial growth in three different types of RMS bedding, as well as in sand, when samples were experimentally inoculated with Escherichia coli and Klebsiella pneumoniae. Two 3-day trials were conducted, during which treatments included inoculating bedding samples with E. coli and K. pneumoniae, as well as no inoculation. The trial was repeated 3 times for each bedding sample on each day. Samples were incubated at 15°C for 3 d and bacterial counts were measured every day. After inoculation, there was no significant K. pneumoniae or E. coli growth phase during the trial in those ...
Source: Canadian Journal of Veterinary Research - January 15, 2024 Category: Veterinary Research Authors: Annie Fr échette Myl ène Généreux Gilles Fecteau Caroline C ôté Simon Dufour Source Type: research

Retrospective study of perioperative antimicrobial use in horses undergoing elective laparoscopy at a single institution
The objective of this study was to evaluate practices associated with perioperative antimicrobial use in equine elective laparoscopy at a single institution over a 21-year period and to determine whether antimicrobial therapy influenced the occurrence of postoperative complications. Medical records of horses that underwent elective laparoscopy at a teaching hospital from January 2000 to September 2021 were reviewed. Data obtained included signalment, surgeon, type and duration of procedure, perioperative antimicrobial use, and intraoperative and postoperative complications. Exact univariate logistic regression was used to ...
Source: Canadian Journal of Veterinary Research - January 15, 2024 Category: Veterinary Research Authors: Lucciana Recchi Nicola Cribb Nathalie C ôté Marie-Soleil Dubois Judith Koenig Alexander Valverde Gabrielle Monteith Source Type: research

Development and validation of a farm- and province-level swine flow simulation model using discrete events and Ontario swine farm and provincial input data
Can J Vet Res. 2024 Jan;88(1):3-11.ABSTRACTInfectious disease events can cause disruptions in service-based and agricultural industries. The list of possible events is long and varies from the incursion or emergence of a reportable animal pathogen to the recently documented interruptions caused by the COVID-19 pandemic. There is a need to develop models that can determine the impact of pathogens and mitigation measures on populations that are not directly affected by the pathogen in the case of a reportable disease, particularly when the health and welfare of these populations could be affected due to resulting disruptions...
Source: Canadian Journal of Veterinary Research - January 15, 2024 Category: Veterinary Research Authors: Maggie Henry Wade McDonald Robert M Friendship Amy L Greer Zvonimir Poljak Source Type: research

Metabolic response of broiler chickens to different doses of < em > Enterococcus faecium < /em > ICIS 96 in the diet
The objectives of this study were to identify the effect of various doses of Enterococcus faecium ICIS 96 added to the diet of broiler chickens on their metabolism and to evaluate the productive qualities of the chickens when different doses are used. The experiment was carried out on 72 Cobb-500 cross broiler chickens, divided into 3 groups. Chickens in the first group received a suspension of E. faecium ICIS 96 containing 1 × 108 cells in 1 mL of sterile saline, at a dose of 0.1 mL per 1 kg of live weight. The second group of chickens received a double dose of enterococcus, that is 0.2 mL of the suspension per 1 kg of l...
Source: Canadian Journal of Veterinary Research - January 15, 2024 Category: Veterinary Research Authors: Elena Kochkina Alfia Andreeva Alexey Torshkov Veronika Dymova Oleg Altynbekov Mariia Sycheva Source Type: research