Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation
Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass s... (Source: BMC Research Notes)
Source: BMC Research Notes - September 30, 2023 Category: Research Authors: Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak and Robert A. Koza Tags: Research Note Source Type: research

Piloting a forced-choice task to elicit treatment preferences in geographic atrophy
Geographic Atrophy (GA) is the advanced form of the non-neovascular ( ‘dry’) type of age-related macular degeneration (AMD) and responsible for one-quarter of legal blindness in the UK. New therapies delivered ... (Source: BMC Research Notes)
Source: BMC Research Notes - September 30, 2023 Category: Research Authors: Jamie Enoch, Arevik Ghulakhszian, Mandeep Sekhon, David P. Crabb, Deanna J. Taylor and Christiana Dinah Tags: Research Note Source Type: research

Evaluation of the frequency of ABO and Rh-Hr blood-group systems in different acquired cataracts type
This study evaluated the relationship between acquired cataract ’s different types and the ABO and Rh blood classes. (Source: BMC Research Notes)
Source: BMC Research Notes - September 30, 2023 Category: Research Authors: Reza Jafari, Hanieh Ahmadi, Samira Chaibakhsh, Fatemeh Rostamian Motlagh, Samira Heydarian, Ahmad Ahmadzadeh Amiri, Asadollah Farrokhfar, Ghasem Rostami and Mahdi Abounoori Tags: Research Note Source Type: research

Development and application of a real-time polymerase chain reaction assay to detect lumpy skin disease virus belonging to the Kenyan sheep and goat pox group
Lumpy skin disease (LSD) outbreaks in Southeast and South Asia are attributed to different lineages of LSD virus (LSDV). Variants belonging to the novel recombinant cluster 2.5 circulate in China and Thailand,... (Source: BMC Research Notes)
Source: BMC Research Notes - September 30, 2023 Category: Research Authors: Alexander Sprygin, Ali Mazloum, Antoinette Van Schalkwyk, Alena Krotova, Olga Bydovskaya, Larisa Prokhvatilova and Ilya Chvala Tags: Research Note Source Type: research

I got frightened and felt strange. I even cried a lot after the diagnosis; the experiences on the screening and management of gestational diabetes mellitus among diagnosed women
Understanding the experiences of women diagnosed with Gestational Diabetes Mellitus (GDM) can improve screening, management, and postpartum care. Therefore, this study sought to investigate experiences on the ... (Source: BMC Research Notes)
Source: BMC Research Notes - September 29, 2023 Category: Research Authors: Abdulai Abubakari, Mohammed Bukari, Hawa Malechi, Humphrey Garti and Faith Agbozo Tags: Research note Source Type: research

Effects of levothyroxine replacement therapy on insulin resistance in patients with untreated primary hypothyroidism
This study investigated the effects of levothyroxine replacement therapy on insulin resistance, lipid profile, and thyroid function in patients with untreated primary hypothyroidism. 105 patients with hypothyr... (Source: BMC Research Notes)
Source: BMC Research Notes - September 29, 2023 Category: Research Authors: Alireza Ostadrahimi, Taher Manzari, Sahar Gohari-Lasaki, Helda Tutunchi, Majid Mobasseri, Vahideh Sadra and Farzad Najafipour Tags: Research Note Source Type: research

Group comparisons of the individual electroretinogram time trajectories for the ascending limb of the b-wave using a raw and registered time series
The electroretinogram is a clinical test commonly used in the diagnosis of retinal disorders with the peak time and amplitude of the a- and b-waves used as the main indicators of retinal function. However, sub... (Source: BMC Research Notes)
Source: BMC Research Notes - September 29, 2023 Category: Research Authors: Marek Brabec, Paul A. Constable, Dorothy A. Thompson and Fernando Marmolejo-Ramos Tags: Research Note Source Type: research

Detection of Parvovirus B19 genome in human heart tissue samples
Identifying viral genomes in human heart tissues is critical for disease diagnosis and assessment of cardiovascular damage. Human heart tissue samples obtained during a biopsy procedure are routinely used to t... (Source: BMC Research Notes)
Source: BMC Research Notes - September 29, 2023 Category: Research Authors: Anna Kloc, Kenneth S. Campbell and Yarida A. Urbina Espinoza Tags: Research Note Source Type: research

Solving singularly perturbed fredholm integro-differential equation using exact finite difference method
In this paper, a numerical scheme is designed for solving singularly perturbed Fredholm integro-differential equation. The scheme is constructed via the exact (non-standard) finite difference method to approxi... (Source: BMC Research Notes)
Source: BMC Research Notes - September 28, 2023 Category: Research Authors: Solomon Regasa Badeye, Mesfin Mekuria Woldaregay and Tekle Gemechu Dinka Tags: Research Note Source Type: research

Burden and predictors of diabetic kidney disease in an adult Ugandan population with new-onset diabetes
Despite the growing evidence of diabetic kidney disease (DKD) in adult patients with long-standing diabetes in sub-Saharan Africa, data on its burden and correlates in adult African patients with new-onset dia... (Source: BMC Research Notes)
Source: BMC Research Notes - September 28, 2023 Category: Research Authors: Davis Kibirige, Isaac Sekitoleko and William Lumu Tags: Research Note Source Type: research

Cross-cultural adaptation of the Parental Perceptions of Children ’s Exposure to Tobacco Smoke instrument to the Brazilian context
To perform a cross-cultural adaptation of the Parental Perceptions of Children ’s Exposure to Tobacco Smoke (PPE) instrument to the Brazilian context. (Source: BMC Research Notes)
Source: BMC Research Notes - September 26, 2023 Category: Research Authors: Maria Alice Santos Tavares, Vicki Myers, Leandro Alberto Calazans Nogueira and Agnaldo Jos é Lopes Tags: Research Note Source Type: research

In vivo direct cell-penetrating peptide mediated protein transduction system in Acyrthosiphon pisum
The principal delivery method for CRISPR-based genome editing in insects is now based on microinjection into single cells or embryos. The direct protein transduction systems cannot be employed in aphids becaus... (Source: BMC Research Notes)
Source: BMC Research Notes - September 25, 2023 Category: Research Authors: Aya Takenaka, Harutomo Konno and Shingo Kikuta Tags: Research Note Source Type: research

MEGADOCK-on-Colab: an easy-to-use protein –protein docking tool on Google Colaboratory
Since the advent of ColabFold, numerous software packages have been provided with Google Colaboratory-compatible ipynb files, allowing users to effortlessly test and reproduce results without the need for loca... (Source: BMC Research Notes)
Source: BMC Research Notes - September 22, 2023 Category: Research Authors: Masahito Ohue Tags: Research Note Source Type: research

Euthymia scale as a protective factor for depressive symptoms: a one-year follow-up longitudinal study
This study aimed to examine whether a high score on the euthymia scale (ES) predicts a low incidence of depressive symptoms one year later. (Source: BMC Research Notes)
Source: BMC Research Notes - September 22, 2023 Category: Research Authors: Natsu Sasaki and Daisuke Nishi Tags: Research Note Source Type: research

Novel genetic variants data for adaptation to hypoxia in native chickens
The genomic response and the role of genetic variants in hypoxia condition are always interesting issues about adaption pathways at genomic level. Herein, we carried out a comparative genomic study between hig... (Source: BMC Research Notes)
Source: BMC Research Notes - September 21, 2023 Category: Research Authors: Atieh Moradi, Hamed Kharrati-Koopaee, Morteza Fardi, Mehdi Farahmandzadeh and Fatemeh Nowroozi Tags: Data Note Source Type: research