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Enhanced productivity and stability of PRV in recombinant ST-Tret1 cells
In this study, we demonstrated that productivity and stability of PRV proliferated in recombinant ST cells with stable expression of Tret1 were enhanced. As a result, a five-fold increase of intracellular trehalose amount was observed, and the significant increase of progeny viral titer was achieved in recombinant cells with the addition of 20 mM trehalose. Particularly, after storage for 8 weeks at 20 °C, the loss of viral titer was 0.8 and 1.7 lgTCID50/mL lower than the control group with or without the addition of trehalose. Additionally, the freeze-thaw resistance at -20 °C and -70 °C of PRV was significantly enhanc...
Source: Biologicals : Journal of the International Association of Biological Standardization - July 13, 2023 Category: Biotechnology Authors: Yue Xu Xi Bao Li Chen Tenghan Zhuang Yang Xu Lei Feng Source Type: research

Enhanced productivity and stability of PRV in recombinant ST-Tret1 cells
In this study, we demonstrated that productivity and stability of PRV proliferated in recombinant ST cells with stable expression of Tret1 were enhanced. As a result, a five-fold increase of intracellular trehalose amount was observed, and the significant increase of progeny viral titer was achieved in recombinant cells with the addition of 20 mM trehalose. Particularly, after storage for 8 weeks at 20 °C, the loss of viral titer was 0.8 and 1.7 lgTCID50/mL lower than the control group with or without the addition of trehalose. Additionally, the freeze-thaw resistance at -20 °C and -70 °C of PRV was significantly enhanc...
Source: Biologicals : Journal of the International Association of Biological Standardization - July 13, 2023 Category: Biotechnology Authors: Yue Xu Xi Bao Li Chen Tenghan Zhuang Yang Xu Lei Feng Source Type: research

Enhanced productivity and stability of PRV in recombinant ST-Tret1 cells
In this study, we demonstrated that productivity and stability of PRV proliferated in recombinant ST cells with stable expression of Tret1 were enhanced. As a result, a five-fold increase of intracellular trehalose amount was observed, and the significant increase of progeny viral titer was achieved in recombinant cells with the addition of 20 mM trehalose. Particularly, after storage for 8 weeks at 20 °C, the loss of viral titer was 0.8 and 1.7 lgTCID50/mL lower than the control group with or without the addition of trehalose. Additionally, the freeze-thaw resistance at -20 °C and -70 °C of PRV was significantly enhanc...
Source: Biologicals : Journal of the International Association of Biological Standardization - July 13, 2023 Category: Biotechnology Authors: Yue Xu Xi Bao Li Chen Tenghan Zhuang Yang Xu Lei Feng Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines
We present here a protocol to establish the purity of virus stock using the live attenuated vaccine candidates ASFV-G-ΔMGF, ASFV-G-Δ9 GLΔUK and ASFV-G-ΔI177L. Procedures described here includes inoculation in susceptible pigs followed by the assessment of the obtained material by differential qPCRs that allows the identification of vaccine virus from ASFV field isolates. This protocol is proposed as a model to ensure that master seed virus stock used for vaccine production does not contain residual parental virulent virus. Procedures described here includes a passage in susceptible pigs followed by the assessment of th...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 5, 2023 Category: Biotechnology Authors: Lauro Velazquez-Salinas Elizabeth Ramirez-Medina Ayushi Rai Sarah Pruitt Elizabeth A Vuono Nallely Espinoza Cyril G Gay Steve Witte Douglas P Gladue Manuel V Borca Source Type: research