Quantification of sweat urea in diabetes using electro-optical technique

OBJECTIVE: To develop a device for quantifying sweats urea concentration. APPROACH: The proposed optical device uses light source, light sensor with time and intensity controlled operation. Sweat samples are collected from a group of volunteers belonging to control and diabetes. After sedimentation and suitable pre-processing, sweat samples are irradiated by primary colour light sources operated sequentially. Reflected light intensity is used to compute the sweat urea concentration. At first the test is performed using aqueous solution with known urea concentration. After ascertaining the correctness of the device, the same test procedure is repeated for sweat samples collected from 90 volunteers (30 per group) belonging to controls, type II diabetes less than 5 years and type II diabetes for more than 5 years. MAIN RESULTS: Computed urea concentration when compared with standard lab techniques like UV-visible absorption spectroscopy and colorimeter, a correlation of 98% with er...
Source: Physiological Measurement - Category: Physiology Authors: Source Type: research