MAnorm2 for quantitatively comparing groups of ChIP-seq samples [METHOD]

We present MAnorm2, a new computational tool for quantitatively comparing groups of ChIP-seq samples. MAnorm2 employs a hierarchical strategy for normalization of ChIP-seq data and assesses within-group variability of ChIP-seq signals based on an empirical Bayes framework. In this framework, MAnorm2 allows for abundant differential ChIP-seq signals between groups of samples as well as very different global within-group variability between groups. Using a number of real ChIP-seq data sets, we observed that MAnorm2 clearly outperformed existing tools for differential ChIP-seq analysis, especially when the groups of samples being compared had distinct global within-group variability.

Source: Genome Research - November 18, 2020 Category: Genetics & Stem Cells Authors: Tu, S., Li, M., Chen, H., Tan, F., Xu, J., Waxman, D. J., Zhang, Y., Shao, Z. Tags: METHOD Source Type: research