XACT-Seq Comprehensively Defines the Promoter-Position and Promoter-Sequence Determinants for Initial-Transcription Pausing

Winkelman et  al. report a protein-DNA photocrosslinking method that provides single-nucleotide-resolution readout of RNA polymerase active-center position relative to DNA and enables analysis of initial-transcription pausing. Analysis of 411 (∼4,000,000) promoter sequences defines positional determinants and sequence determinants for initial-transcription pausing by bacterial RNA polymerase in vitro and in vivo.

Source: Molecular Cell - August 2, 2020 Category: Cytology Authors: Jared T. Winkelman, Chirangini Pukhrambam, Irina O. Vvedenskaya, Yuanchao Zhang, Deanne M. Taylor, Premal Shah, Richard H. Ebright, Bryce E. Nickels Tags: Article Source Type: research