Electric Field –Induced Release and Measurement (EFIRM)

Electric field –induced release and measurement (EFIRM) is a novel, plate-based, liquid biopsy platform capable of detecting circulating tumor DNA containing EGFR mutations directly from saliva and plasma in both early- and late-stage patients with non–small-cell lung cancer. We investigated the properties of the target molecule for EFIRM and determined that the platform preferentially detects single-stranded DNA molecules. We then investigated the properties of the EFIRM assay and determined the linearity, linear range, precision, and limit of detection for six different EGFR variants (the four most com mon g.

Source: Journal of Molecular Diagnostics - May 31, 2020 Category: Pathology Authors: Michael Tu, Jordan Cheng, Yi-Lin Chen, Wen-Chien Jea, Wan-Li Chen, Chien-Jung Chen, Chung-Liang Ho, Wei-Lun Huang, Chien-Chung Lin, Wu-Chou Su, Qianlin Ye, Josh Deignan, Wayne Grody, Feng Li, David Chia, Fang Wei, Wei Liao, David T.W. Wong, Charles M. Str Tags: Regular article Source Type: research