Structures and Strategies of Anti-CRISPR-Mediated Immune Suppression.

Structures and Strategies of Anti-CRISPR-Mediated Immune Suppression.
Annu Rev Microbiol. 2020 Jun 05;:
Authors: Wiegand T, Karambelkar S, Bondy-Denomy J, Wiedenheft B
Abstract
More than 50 protein families have been identified that inhibit CRISPR (clustered regularly interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we analyze the available anti-CRISPR (Acr) structures and describe common themes and unique mechanisms of stoichiometric and enzymatic suppressors of CRISPR-Cas. Stoichiometric inhibitors often function as molecular decoys of protein-binding partners or nucleic acid targets, while enzymatic suppressors covalently modify Cas ribonucleoprotein complexes or degrade immune signaling molecules. We review mechanistic insights that have been revealed by structures of Acrs, discuss some of the trade-offs associated with each of these strategies, and highlight how Acrs are regulated and deployed in the race to overcome adaptive immunity. Expected final online publication date for the Annual Review of Microbiology, Volume 74 is September 8, 2020. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.
PMID: 32503371 [PubMed - as supplied by publisher]

Source: Annual Review of Microbiology - June 4, 2020 Category: Microbiology Authors: Wiegand T, Karambelkar S, Bondy-Denomy J, Wiedenheft B Tags: Annu Rev Microbiol Source Type: research