Enzymatic and chemical-based methods to inactivate endogenous blood ribonucleases for nucleic acid diagnostics

There are ongoing research efforts into simple and low-cost point-of-care (POC) nucleic acid amplification tests (NATs) addressing widespread diagnostic needs in resource-limited clinical settings. Nucleic acid testing for RNA targets in blood specimens typically requires sample preparation that inactivates robust blood ribonucleases (RNases) that can rapidly degrade exogenous RNA. Most NATs rely on decades-old methods that lyse pathogens and inactivate RNases with high concentrations of guanidinium salts.

Source: Journal of Molecular Diagnostics - May 20, 2020 Category: Pathology Authors: Andrew T. Bender, Benjamin P. Sullivan, Lorraine Lillis, Jonathan D. Posner Source Type: research