Therapeutic Antibody Engineering and Selection Strategies.

Therapeutic Antibody Engineering and Selection Strategies.
Adv Biochem Eng Biotechnol. 2019 Nov 28;:
Authors: Ministro J, Manuel AM, Goncalves J
Abstract
Antibody drugs became an increasingly important element of the therapeutic landscape. Their accomplishment has been driven by many unique properties, in particular by their very high specificity and selectivity, in contrast to the off-target liabilities of small molecules (SMs). Antibodies can bring additional functionality to the table with their ability to interact with the immune system, and this can be further manipulated with advances in antibody engineering.The expansion of strategies related to discovery technologies of monoclonal antibodies (mAbs) (phage display, yeast display, ribosome display, bacterial display, mammalian cell surface display, mRNA display, DNA display, transgenic animal, and human B cell derived) opened perspectives for the screening and the selection of therapeutic antibodies for, theoretically, any target from any kind of organism. Moreover, antibody engineering technologies were developed and explored to obtain chosen characteristics of selected leading candidates such as high affinity, low immunogenicity, improved functionality, improved protein production, improved stability, and others. This chapter contains an overview of discovery technologies, mainly display methods and antibody humanization methods for the selection of therapeutic humanized and ...

Source: Advances in Biochemical Engineering Biotechnology - November 27, 2019 Category: Biotechnology Authors: Ministro J, Manuel AM, Goncalves J Tags: Adv Biochem Eng Biotechnol Source Type: research