On the catalytic mechanism of bacteriophage endolysins: Opportunities for engineering

Publication date: Available online 31 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Michael J. Love, Gayan S. Abeysekera, Andrew C. Muscroft-Taylor, Craig Billington, Renwick C.J. DobsonAbstractBacteriophage endolysins have the potential to be a long-term antibacterial replacement for antibiotics. The exogenous application of endolysins on some bacteria results in rapid cell lysis. The prospects for endolysins are furthered by the ability to engineer them; novel endolysins can be developed with optimised stability, specificity, and lytic function. But the success of endolysin engineering and application requires a comprehensive understanding of the relationship between the enzymes biochemical, biophysical and bacteriolytic properties. Here, we examine their catalytic mechanisms, opportunities for developing novel endolysins, and highlight areas where a better understanding would support their long-term success as antibacterial agents.

Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research