1006 - key roles of hoxa1 in hscs and myelodysplastic syndromes

Homeobox a1 (Hoxa1) has 2 different isoforms: a full-length homeodomain-containing Hoxa1 (Hoxa1-FL) and a truncated Hoxa1 (Hoxa1-T), which is spliced within exon 1 of Hoxa1-FL and lacks the homeodomain. Hoxa1-FL and Hoxa1-T are differentially expressed in distinct populations of hematopoietic cells. To examine their roles in hematopoiesis, we retrovirally overexpressed WT-Hoxa1 (which generates both isoforms) or Hoxa1-T in murine bone marrow (BM). We also generated a mutant Hoxa1 (MUT-Hoxa1), which expresses Hoxa1-FL but not Hoxa1-T, by site-directed mutagenesis.
Source: Experimental Hematology - Category: Hematology Authors: Source Type: research