Qualification of a surface plasmon resonance assay to determine binding of IgG-type antibodies to complement component C1q.

Qualification of a surface plasmon resonance assay to determine binding of IgG-type antibodies to complement component C1q.
Biologicals. 2019 Aug 21;:
Authors: Jovic M, Cymer F
Abstract
Interaction of therapeutic antibodies with complement component C1q are frequently part of pharmaceutical characterization and production process comparability studies. Assays currently used to assess this interaction and/or the activation of the complement cascade are often cumbersome, time consuming or imprecise. We here report the further development, successful qualification and suitability evaluation of an SPR-based C1q binding assay for the characterization of IgG-C1q interactions. We evaluate different IgG subtypes and well-described mutants of IgG1. The assay offers a suitable alternative for extended characterization of interactions of IgG1 and IgG3 with complement component C1q.
PMID: 31445761 [PubMed - as supplied by publisher]

Source: Biologicals : Journal of the International Association of Biological Standardization - August 20, 2019 Category: Biotechnology Authors: Jovic M, Cymer F Tags: Biologicals Source Type: research