055 The leukocyte compartment of subcutaneous white adipose tissue: major differences as to skin and blood

In contrast to the well-characterized leukocytic compartment in human skin, relatively little is known about the one residing in the fat layer beneath. To address this issue, we collected thigh-liposuction-derived subcutaneous white adipose tissue (SWAT; n=14) from healthy young women, normal skin (n=7) and blood (n=7), isolated the leukocytes from these compartments, characterized them by FACS and subjected them to cytokine stimulation assays. In addition, we performed in situmulticolor immunofluorescence stainings.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Adaptive and Auto-Immunity Source Type: research