T7 exo-mediated FRET-breaking combined with DSN –RNAse–TdT for the detection of microRNA with ultrahigh signal-amplification

Analyst, 2019, Advance Article DOI: 10.1039/C9AN00303G, CommunicationVan Thang Nguyen, Binh Huy Le, Young Jun Seo A DSN –RNAse–TdT–T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity—the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry

Source: RSC - Analyst latest articles - April 16, 2019 Category: Chemistry Authors: Van Thang Nguyen Source Type: research