High efficiency biotransformation of bisphenol A in a fluidized bed reactor using stabilized laccase in porous silica

Publication date: Available online 28 March 2019Source: Enzyme and Microbial TechnologyAuthor(s): Mingyue Piao, Donglei Zou, Xianghao Ren, Song Gao, Chuanyu Qin, Yunxian PiaoAbstractWe developed a high efficiency bisphenol A (BPA) treating system in a fluidized bed reactor (FBR) using stabilized laccase (Lac) in mesoporous silica (SER/Lac). The SER/Lac, prepared by cross linking of each enzymes inside the porous silica using glutaraldehyde, presented improved stability than the free Lac over various pH and temperatures with shaking. When the SER/Lac was used for BPA biotransformation in a batch reaction, higher efficiency was achieved than the free or adsorbed Lac (ADS/Lac). Also, the SER/Lac presented better reusability compared to ADS/Lac, and it could be reused for three times without decrease of biotransformation efficiency, and half of it was remained after ten times use. Due to great stability and robustness property, SER/Lac was successfully applied for high efficiency and continuous BPA treatment in FBR with better performance than the batch reaction.Graphical abstractBiotransformation of BPA in FBR

Source: Enzyme and Microbial Technology - March 30, 2019 Category: Biotechnology Source Type: research