Development of Cell Analysis Software to Evaluate Fibroblastic Changes in Cultivated Corneal Endothelial Cells for Quality Control

Purpose: To develop software to evaluate the fibroblastic morphological changes in cultured human corneal endothelial cells (HCECs) as a quality control measure for use in tissue engineering therapy. Methods: Software was designed to recognize cell borders, to approximate cell shape as an ellipse, and to calculate the aspect ratio of the ellipse as an indicator of severity of the fibroblastic morphological change. Using the designed software, 60 phase contrast images of polygonal HCECs and 60 phase contrast images of fibroblastic HCECs were analyzed. The correlations of the aspect ratio and other parameters (cell density, percentage of cells surrounded by 6 cells, and coefficient of variation) were evaluated. Results: Cell shapes were recognized based on phase contrast images and were approximated as ellipses by software. The average aspect ratio was significantly higher (34.9% ± 6.1%) in fibroblastic HCECs than in polygonal HCECs (24.4% ± 2.3%) (P
Source: Cornea - Category: Opthalmology Tags: Basic Investigation Source Type: research
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