Crystallization and preliminary X-ray crystallographic analysis of an artificial molten-globular-like triosephosphate isomerase protein of mixed phylogenetic origin

In this report, the ccTIM protein was successfully crystallized using the microbatch-under-oil method and a full X-ray diffraction data set was collected to 2.2 Å resolution using a synchrotron-radiation source. The crystals belonged to space group C2221, with unit-cell parameters a = 107.97, b = 187.21, c = 288.22 Å. Matthews coefficient calculations indicated the presence of six dimers in the asymmetric unit, with an approximate solvent content of 46.2%.

Source: Acta Crystallographica Section F - October 25, 2014 Category: Biochemistry Authors: Goyal, V.D.Yadav, P.Kumar, A.Ghosh, B.Makde, R.D. Tags: protein engineering triosephosphate isomerase consensus design molten globule crystallization communications Source Type: research