Stachybotrychromenes A –C: novel cytotoxic meroterpenoids from Stachybotrys sp.

AbstractIn the course of gaining new insights into the secondary metabolite profile of variousStachybotrys strains, in particular concerning triprenyl phenol-like compounds, so far, unknown metabolites with analogous structural features were discovered. Three novel meroterpenoids containing a chromene ring moiety, namely stachybotrychromenes A –C, were isolated from solid culture of the filamentous fungusStachybotrys chartarum DSMZ 12880 (chemotype S). Their structures were elucidated by means of comprehensive spectroscopic analysis (1D and 2D NMR, ESI-HRMS, and CD) as well as by comparison with spectroscopic data of structural analogues described in literature. Stachybotrychromenes A and B exhibited moderate cytotoxic effects on HepG2 cells after 24  h with corresponding IC50 values of 73.7 and 28.2  μM, respectively. Stachybotrychromene C showed no significant cytotoxic activity up to 100 μM. Moreover, it is noteworthy that stachybotrychromenes A–C are produced not only byS. chartarum chemotype S but alsoS. chartarum chemotype A andStachybotrys chlorohalonata.

Source: Mycotoxin Research - August 1, 2018 Category: Toxicology Source Type: research