Expression profile of microRNAs in bronchoalveolar lavage fluid of rats as predictors for smoke inhalation injury

Publication date: Available online 20 August 2018Source: BurnsAuthor(s): Peixin Xiao, Shuli Sun, Juan Cao, Jing Wang, Helin Li, Shike Hou, Hui Ding, Ziquan Liu, Yifei Fang, Song Bai, Xiaojing Qin, Fei Yu, Jinyang Liu, Xue Wang, Qi Lv, Haojun FanAbstractSmoke inhalation injury (SII) is an independent risk factor for morbidity and mortality in patients with severe burns, however, the underlying mechanisms of SII are still not fully understood. In our study, we established an advanced rat model of SII based on the previous work, and explored the dynamic changes of pathophysiology and inflammatory factors during 28 days post SII. We also measured the different expressions of miRNAs in bronchoalveolar lavage fluid (BALF) between SII and normal control rats by miRNA microarray. At 1 day after smoke inhalation, the histopathological results exhibited inflammatory exudates in the lung tissue with significant edema. As time went on, the lung injuries gradually appeared at alveolar septum thickening and alveolar collapse, which suggested that it further induced damage to lung parenchyma by smoke inhalation. Particularly, the collagen deposition indicating pulmonary fibrosis happened at 28 days post-injury. Plasma IL-6 and TNF-a were significantly increased after 1 day of smoke inhalation. Plasma IL-10, BALF TNF-α and IL-10 were significantly increased after 2 days of smoke inhalation. By extending the observation time, the levels of plasma IL-6, BALF TNF-a and...

Source: Burns - August 21, 2018 Category: Dermatology Source Type: research