A class-specific artificial receptor-based on molecularly imprinted polymer-coated quantum dot centers for the detection of signaling molecules, N-acyl-homoserine lactones present in gram-negative bacteria.

A class-specific artificial receptor-based on molecularly imprinted polymer-coated quantum dot centers for the detection of signaling molecules, N-acyl-homoserine lactones present in gram-negative bacteria.
Anal Chim Acta. 2018 Nov 15;1031:134-144
Authors: Habimana JD, Ji J, Pi F, Karangwa E, Sun J, Guo W, Cui F, Shao J, Ntakirutimana C, Sun X
Abstract
Herein, a novel class-specific artificial receptor-based on molecularly imprinted polymer (MIP)-coated quantum dots (QDs@MIP) was synthesized, characterized, and used for the detection and quantification of the bacterial quorum signaling molecules N-acyl-homoserine lactones (AHLs), a class of autoinducers from Gram-negative bacteria. The QDs@MIP was prepared by surface imprinting technique under controlled conditions using CdSe/ZnS QDs as the signal transducing material. The synthesis of the QDs@MIP was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction analysis, and fluorescence spectroscopy. After template elution, the obtained cavities sensitively and selectively recognized the target AHLs of interest. The fluorescence intensity of the QDs@MIP was significantly quenched compared to the control non-imprinted polymer (QDs@NIP) upon exposure to different AHL concentrations. It also had a good linearity in the range from 2 to 18 nM along with a detection limit of 0.66, 0.54, 0.88, 0.72 and 0.68 ...

Source: Analytica Chimica Acta - August 19, 2018 Category: Chemistry Authors: Habimana JD, Ji J, Pi F, Karangwa E, Sun J, Guo W, Cui F, Shao J, Ntakirutimana C, Sun X Tags: Anal Chim Acta Source Type: research