Affinity-based separation methods for the study of biological interactions: The case of peroxisome proliferator-activated receptors in drug discovery

Publication date: Available online 17 February 2018Source: MethodsAuthor(s): Caterina Temporini, Gloria Brusotti, Giorgio Pochetti, Gabriella Massolini, Enrica CalleriAbstractAffinity-based methods using immobilized proteins are important approaches for understanding the interactions between small molecules and biological targets. This review is intended to provide an overview of different affinity based separation methods that have been applied to the study of peroxisome proliferator activated receptors (PPARs). The screening of compound to increase screening rates for synthetic and natural ligands of PPAR are reported. Pros and cons of the approaches in ligand discovery initiatives are discussed.

Source: Methods - July 15, 2018 Category: Molecular Biology Source Type: research