Targeted analysis of microbial-generated phenolic acid metabolites derived from grape flavanols by gas chromatography-triple quadrupole mass spectrometry

Publication date: Available online 20 June 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Eileen Carry, Danyue Zhao, Ilaria Mogno, Jeremiah Faith, Lap Ho, Tom Villani, Harna Patel, Giulio M. Pasinetti, James E. Simon, Qingli WuAbstractGrape-derived products contain a wide array of bioactive phenolic compounds which are of significant interest to consumers and researchers for their multiple health benefits. The majority of bioavailable grape polyphenols, including the most abundant flavan-3-ols, i.e. (+)-catechin and (-)-epicatechin, undergo extensive microbial metabolism in the gut, forming metabolites that can be highly bioavailable and bioactive. To gain a better understanding in microbial metabolism of grape polyphenols and to identify bioactive metabolites, advanced analytical methods are needed to accurately quantitate microbial-derived metabolites, particularly at trace levels, in addition to their precursors. This work describes the development and validation of a high-throughput, sensitive and reproducible GC-QqQ/MS method operated under MRM mode that allowed the identification and quantification of 16 phenolic acid metabolites, along with (+)-catechin and (-)-epicatechin, in flavanol-enriched broth samples anaerobically fermented with human intestinal bacteria. Excellent sensitivity was achieved with low limits of detection and low limits of quantification in the range of 0.24 – 6.18 ng/mL and 0.480 – 12.37 ng/mL, respectively. With t...

Source: Journal of Pharmaceutical and Biomedical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research