Glycobiotechnology of the Insect Cell-Baculovirus Expression System Technology.

Glycobiotechnology of the Insect Cell-Baculovirus Expression System Technology. Adv Biochem Eng Biotechnol. 2018 Jun 10;: Authors: Palomares LA, Srivastava IK, Ramírez OT, Cox MMJ Abstract The insect cell-baculovirus expression system technology (BEST) has a prominent role in producing recombinant proteins to be used as research and diagnostic reagents and vaccines. The glycosylation profile of proteins produced by the BEST is composed predominantly of terminal mannose glycans, and, in Trichoplusia ni cell lines, core α3 fucosylation, a profile different to that in mammals. Insects contain all the enzymatic activities needed for complex N- and O-glycosylation and sialylation, although few reports of complex glycosylation and sialylation by the BEST exist. The insect cell line and culture conditions determine the glycosylation profile of proteins produced by the BEST. The promoter used, dissolved oxygen tension, presence of sugar precursors, bovine serum or hemolymph, temperature, and the time of harvest all influence glycosylation, although more research is needed. The lack of activity of glycosylation enzymes possibly results from the transcription regulation and stress imposed by baculovirus infection. To solve this limitation, the glycosylation pathway of insect cells has been engineered to produce complex sialylated glycans and to eliminate α3 fucosylation, either by generating transgenic cell lines or by using baculovirus vec...
Source: Advances in Biochemical Engineering Biotechnology - Category: Biotechnology Authors: Tags: Adv Biochem Eng Biotechnol Source Type: research