Novel epoxy-silica nanoparticles to develop non-enzymatic colorimetric probe for analytical immuno/bioassays.

Novel epoxy-silica nanoparticles to develop non-enzymatic colorimetric probe for analytical immuno/bioassays.
Anal Chim Acta. 2018 Oct 22;1028:77-85
Authors: Dixit CK, Bhakta S, Macharia J, Furtado J, Suib SL, Rusling JF
Abstract
We have developed a novel method to develop epoxy silica nanoparticles (EfSiNP) in a single pot. High surface coverage of epoxy functional groups between 150 and 57000 molecules per particles (∼1013-1016 molecules/mL of 200 nm EfSiNPs) was achieved for different preparation conditions. We then created a red colored probe by conjugating Fuchsin dye to the epoxy functionalities of EfSINPs. Anti-mouse IgG was co-immobilized with Fuchsin and their ratios were optimized for achieving optimum ratios by testing those in functional assays. Dye to antibody ratios were in good negative correlation with a coefficient of -1.00 measured at a confidence level of over 99%. We employed the developed non-enzymatic colorimetric immunonanoprobe for detecting mouse IgG in a direct immunoassay format. We achieved a sensitivity of 427 pg/mL with the assay.
PMID: 29884356 [PubMed - in process]

Source: Analytica Chimica Acta - June 11, 2018 Category: Chemistry Authors: Dixit CK, Bhakta S, Macharia J, Furtado J, Suib SL, Rusling JF Tags: Anal Chim Acta Source Type: research