Aptamer-based microcantilever-array biosensor for profenofos detection.

Aptamer-based microcantilever-array biosensor for profenofos detection.
Anal Chim Acta. 2018 Aug 22;1020:116-122
Authors: Li C, Zhang G, Wu S, Zhang Q
Abstract
Profenofos, a highly poisonous organophosphorus pesticide, has been widely used in agricultural production. These pesticide residues have seriously influenced food security and threatened human health, and new methods with high sensitivity are greatly needed to detect profenofos. Here, we developed an aptamer-based microcantilever-array sensor operated in stress mode to detect profenofos, with advantages of being a label-free, highly sensitive, one-step immobilization method capable of quantitative and real-time detection. The microcantilevers were functionalized with a profenofos-specific aptamer (SS2-55), and then the specific binding of profenofos to aptamer induced a deflection of the microcantilever, which was monitored using an optical method in a real-time manner. The microcantilever deflection showed a positive relationship with profenofos concentration, and the detection limit was low to 1.3 ng mL-1 (3.5 nM) for profenofos, which was much lower than other aptamer-based detection methods. The selectivity of the sensor was verified with another organophosphorus pesticide. Additionally, we successfully detected profenofos dissolved in vegetable-soak solution. Our results showed that this aptamer-based microcantilever-array sensor is a convenient and label-free meth...

Source: Analytica Chimica Acta - April 17, 2018 Category: Chemistry Authors: Li C, Zhang G, Wu S, Zhang Q Tags: Anal Chim Acta Source Type: research