High throughput single-cell fate potential assay of murine hematopoietic progenitors in vitro

The advent of single cell transcriptomics has led to the proposal of a number of novel high- resolution models for the hematopoietic system. Testing the predictions generated by such models requires cell fate potential assays of matching, single cell resolution. Here we detail the development of an in vitro high throughput single-cell culture assay using flow-cytometrically-sorted single murine bone-marrow progenitors, that measures their differentiation into any of 5 myeloid lineages. We identify critical parameters for single cell culture outcome, including the choice of sorter nozzle size and pressure, culture media and the coating of culture dishes with extracellular matrix proteins.

Source: Experimental Hematology - February 3, 2018 Category: Hematology Authors: Betsabeh Khoramian Tusi, Merav Socolovsky Tags: Methods and Techniques Source Type: research